Abstract
Tubby-like proteins (TLPs), characterized by a signature tubby domain, are widespread in plants and animals. To date, only plant TLPs involved in multifarious stress responses and male gametophyte development have been identified. However, studies on the molecular functions of plant TLPs are largely unknown. In this investigation, the roles of a TLP from Malus domestica ( Md TLP7) in response to abiotic stresses were characterized by expressing it in Arabidopsis. The expression of wild-type full-length Md TLP7 (FL) significantly increased the stress tolerance of Arabidopsis seedlings to osmotic, salt, cold and heat stress, while the expression of truncated Md TLP7 containing only the tubby domain (Tub) also showed some function. Located on a central α helix surrounded by 12 anti-parallel β strands in the tubby domain, the K 190 /R 192 site may be involved in fixation to the plasma membrane, as shown by 3D homology modelling with animal TLPs. This site might play a crucial role in anti-stress functions since site-directed mutagenesis of Md TLP7 reduced stress tolerance. Subcellular localization showed that Md TLP7 was mainly localized in the plasma membrane in plant cells, suggesting that it might participate in the transduction of stress signals. The results of this study showed that Md TLP7 could improve abiotic stress tolerance not only in bacteria but also in plants. The K 190 /R 192 residues in the tubby domain were not only the plasma membrane binding site of Md TLP7 but also played a key role in stress tolerance. These results may provide a basis for further exploring the mechanism of anti-stress functioning and downstream target genes of plant TLPs.
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Claims 8
Claim · model-written summary
Full-length MdTLP7 expression significantly increased Arabidopsis seedling tolerance to osmotic, salt and cold stress.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- expression of wild-type full-length Md TLP7 (FL)e0
- Outcome
- stress tolerancee0
- Direction
- increasede0
- Qualifier
- osmotic, salt, colde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances abiotic stress tolerance in Arabidopsis”
e0“The expression of wild-type full-length Md TLP7 (FL) significantly increased the stress tolerance of Arabidopsis seedlings to osmotic, salt, cold and heat stress”
Claim · model-written summary
Full-length MdTLP7 expression significantly increased Arabidopsis seedling tolerance to heat stress.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- expression of wild-type full-length Md TLP7 (FL)e0
- Outcome
- stress tolerancee0
- Direction
- increasede0
- Qualifier
- heat stresse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances abiotic stress tolerance in Arabidopsis”
e0“The expression of wild-type full-length Md TLP7 (FL) significantly increased the stress tolerance of Arabidopsis seedlings to osmotic, salt, cold and heat stress”
Claim · model-written summary
Truncated MdTLP7 containing only the tubby domain showed some function in stress tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- truncated Md TLP7 containing only the tubby domain (Tub)e1
- Outcome
- some functione1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances abiotic stress tolerance in Arabidopsis”
e1“while the expression of truncated Md TLP7 containing only the tubby domain (Tub) also showed some function”
Claim · model-written summary
Site-directed mutagenesis of MdTLP7 reduced stress tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- site-directed mutagenesise2
- Gene
- Md TLP7e2
- Outcome
- stress tolerancee2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances abiotic stress tolerance in Arabidopsis”
e2“site-directed mutagenesis of Md TLP7 reduced stress tolerance”
Claim · model-written summary
The K190/R192 site may be involved in fixation of MdTLP7 to the plasma membrane.
- Taxon
- Malus domestica
Malus domesticat1 - Gene
- K 190 /R 192 sitee5
- Outcome
- fixation to the plasma membranee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“a tubby-like protein from Malus domestica (MdTLP7)”
e5“The K 190 /R 192 site may be involved in fixation to the plasma membrane”
Claim · model-written summary
MdTLP7 was mainly localized in the plasma membrane in plant cells.
- Taxon
- ArabidopsisArabidopsist0
- Material
- Md TLP7e3
- Outcome
- mainly localized in the plasma membranee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances abiotic stress tolerance in Arabidopsis”
e3“Subcellular localization showed that Md TLP7 was mainly localized in the plasma membrane in plant cells”
Claim · model-written summary
MdTLP7 improved abiotic stress tolerance in bacteria as well as in plants.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- abiotic stress tolerancee4
- Qualifier
- not only in bacteria but also in plantse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances abiotic stress tolerance in Arabidopsis”
e4“Md TLP7 could improve abiotic stress tolerance not only in bacteria but also in plants”
Claim · model-written summary
The K190/R192 residues in the tubby domain were the plasma membrane binding site and played a key role in stress tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- K 190 /R 192 residuese6
- Outcome
- stress tolerancee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“enhances abiotic stress tolerance in Arabidopsis”
e6“The K 190 /R 192 residues in the tubby domain were not only the plasma membrane binding site of Md TLP7 but also played a key role in stress tolerance”
The same questions in other papers
climate site · tolerance: abiotic · ArabidopsisPositive and negative results
1 in the opposite direction
- positive
transgenic · tolerance: abiotic · ArabidopsisPositive and negative results
18 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
17 papers more in this group
See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.