Abstract
BACKGROUND: Manganese (Mn), an essential element for plants, can be toxic when present in excess. Stylo (Stylosanthes) is a pioneer tropical legume with great potential for Mn tolerance, but its Mn tolerance mechanisms remain poorly understood. RESULTS: In this study, variations in Mn tolerance were observed among nine stylo genotypes. Stylo genotype 'RY5' exhibited the highest Mn tolerance compared to the other tested genotypes, whereas 'TF2001' was a Mn-sensitive genotype. The mechanisms underlying the response of stylo to Mn toxicity were further investigated using these two genotypes with contrasting Mn tolerance. Results showed that stylo genotype RY5 exhibited Mn tolerance superior to that of genotype TF2001, showing lower reductions in leaf chlorophyll concentration, chlorophyll fluorescence parameters, photosynthetic indexes and plant dry weight under Mn toxicity. A label-free quantitative proteomic analysis was conducted to investigate the protein profiles in the leaves and roots of RY5 in response to Mn toxicity. A total of 356 differentially expressed proteins (DEPs) were identified, including 206 proteins from leaves and 150 proteins from roots, which consisted of 71 upregulated, 62 downregulated, 127 strongly induced and 96 completely suppressed proteins. These DEPs were mainly involved in defense response, photosynthesis, carbon fixation, metabolism, cell wall modulation and signaling. The qRT-PCR analysis verified that 10 out of 12 corresponding gene transcription patterns correlated with their encoding proteins after Mn exposure. Finally, a schematic was constructed to reveal insights into the molecular processes in the leaves and roots of stylo in response to Mn toxicity. CONCLUSIONS: These findings suggest that stylo plants may cope with Mn toxicity by enhancing their defense response and phenylpropanoid pathways, adjusting photosynthesis and metabolic processes, and modulating protein synthesis and turnover. This study provides a platform for the future study of Mn tolerance mechanisms in stylo and may lead to a better understanding of the potential mechanisms underlying tropical legume adaptation to Mn toxicity.
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Claims 8
Claim · model-written summary
RY5 had the highest Mn tolerance among nine stylo genotypes, whereas TF2001 was sensitive.
- Taxon
- Stylo
.jpg?width=60)
Stylosanthes guianensise0 - Material
- RY5e0
- Outcome
- Mn tolerancee0
- Direction
- higheste0
- Comparator
- the other tested genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Stylo genotype 'RY5' exhibited the highest Mn tolerance compared to the other tested genotypes, whereas 'TF2001' was a Mn-sensitive genotype.”
Claim · model-written summary
Under Mn toxicity, RY5 had lower reductions in leaf chlorophyll concentration than TF2001.
- Taxon
- styloStylosanthes guianensise0
- Material
- RY5e0
- Treatment
- Mn toxicitye0
- Outcome
- leaf chlorophyll concentratione0
- Direction
- lower reductionse0
- Comparator
- TF2001e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stylo genotype RY5 exhibited Mn tolerance superior to that of genotype TF2001, showing lower reductions in leaf chlorophyll concentration, chlorophyll fluorescence parameters, photosynthetic indexes and plant dry weight under Mn toxicity.”
Claim · model-written summary
Under Mn toxicity, RY5 had lower reductions in chlorophyll fluorescence parameters and photosynthetic indexes than TF2001.
- Taxon
- styloStylosanthes guianensise0
- Material
- RY5e0
- Treatment
- Mn toxicitye0
- Outcome
- chlorophyll fluorescence parameters, photosynthetic indexese0
- Direction
- lower reductionse0
- Comparator
- TF2001e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stylo genotype RY5 exhibited Mn tolerance superior to that of genotype TF2001, showing lower reductions in leaf chlorophyll concentration, chlorophyll fluorescence parameters, photosynthetic indexes and plant dry weight under Mn toxicity.”
Claim · model-written summary
Under Mn toxicity, RY5 had lower reductions in dry weight than TF2001.
- Taxon
- styloStylosanthes guianensise0
- Material
- RY5e0
- Treatment
- Mn toxicitye0
- Outcome
- plant dry weighte0
- Direction
- lower reductionse0
- Comparator
- TF2001e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stylo genotype RY5 exhibited Mn tolerance superior to that of genotype TF2001, showing lower reductions in leaf chlorophyll concentration, chlorophyll fluorescence parameters, photosynthetic indexes and plant dry weight under Mn toxicity.”
Claim · model-written summary
Proteomics identified 356 differentially expressed proteins in RY5 leaves and roots after Mn exposure.
- Taxon
- Stylosanthes
Stylosanthest0 - Material
- RY5e0
- Organ / stage
- leaves and rootse0
- Outcome
- differentially expressed proteins (DEPs)e0
- Value
- 356e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A label-free quantitative proteomic analysis was conducted to investigate the protein profiles in the leaves and roots of RY5 in response to Mn toxicity. A total of 356 differentially expressed proteins (DEPs) were identified, including 206 proteins from leaves and 150 proteins from roots, which consisted of 71 upregulated, 62 downregulated, 127 strongly induced and 96 completely suppressed proteins.”
t0“Stylosanthes response to manganese toxicity”
Claim · model-written summary
The differentially expressed proteins were mainly involved in defense, photosynthesis, carbon fixation, metabolism, cell walls and signaling.
- Taxon
- StylosanthesStylosanthest0
- Outcome
- defense response, photosynthesis, carbon fixation, metabolism, cell wall modulation and signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These DEPs were mainly involved in defense response, photosynthesis, carbon fixation, metabolism, cell wall modulation and signaling.”
t0“Stylosanthes response to manganese toxicity”
Claim · model-written summary
Ten of twelve gene transcription patterns correlated with their encoding proteins after Mn exposure.
- Taxon
- StylosanthesStylosanthest0
- Treatment
- Mn exposuree0
- Outcome
- gene transcription patterns correlated with their encoding proteinse0
- Value
- 10 out of 12e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The qRT-PCR analysis verified that 10 out of 12 corresponding gene transcription patterns correlated with their encoding proteins after Mn exposure.”
t0“Stylosanthes response to manganese toxicity”
Claim · model-written summary
The authors suggest defense and phenylpropanoid enhancement, photosynthetic and metabolic adjustment, and protein turnover modulation as Mn tolerance responses.
- Taxon
- styloStylosanthes guianensise0
- Outcome
- cope with Mn toxicitye0
- Qualifier
- enhancing their defense response and phenylpropanoid pathways, adjusting photosynthesis and metabolic processes, and modulating protein synthesis and turnovere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“stylo plants may cope with Mn toxicity by enhancing their defense response and phenylpropanoid pathways, adjusting photosynthesis and metabolic processes, and modulating protein synthesis and turnover.”
The same questions in other papers
manganese atom · physiology: pigments · Stylosanthes guianensis (stylo)Positive and negative results
2 in the opposite direction
- negative
- negative
manganese atom · growth · Stylosanthes guianensis (stylo)Positive and negative results
2 in the opposite direction
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.