Abstract
RNAi (RNA interference) is an important defense response against virus infection in plants. The core machinery of the RNAi pathway in plants include DCL (Dicer Like), AGO (Argonaute) and RdRp (RNA dependent RNA polymerase). Although involvement of these RNAi components in virus infection responses was demonstrated in Arabidopsis thaliana, their contribution to antiviral immunity in Nicotiana benthamiana, a model plant for plant-pathogen interaction studies, is not well understood. In this study, we investigated the role of N. benthamiana NbAGO2 gene against TMV (Tomato mosaic virus) infection. Silencing of NbAGO2 by transient expression of an hpRNA construct recovered GFP (Green fluorescent protein) expression in GFP-silenced plant, demonstrating that NbAGO2 participated in RNAi process in N. benthamiana. Expression of NbAGO2 was transcriptionally induced by both MeSA (Methylsalicylate acid) treatment and TMV infection. Down-regulation of NbAGO2 gene by amiR-NbAGO2 transient expression compromised plant resistance against TMV infection. Inhibition of endogenous miR403a, a predicted regulatory microRNA of NbAGO2, reduced TMV infection. Our study provides evidence for the antiviral role of NbAGO2 against a Tobamovirus family virus TMV in N. benthamiana, and SA (Salicylic acid) mediates this by induction of NbAGO2 expression upon TMV infection. Our data also highlighted that miR403a was involved in TMV defense by regulation of target NbAGO2 gene in N. Benthamiana.
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Claims 7
Claim · model-written summary
NbAGO2 participated in RNA interference, as silencing restored GFP expression.
- Taxon
- N. benthamiana
Nicotiana benthamianae0 - Treatment
- Silencing of NbAGO2 by transient expression of an hpRNA constructe0
- Gene
- NbAGO2e0
- Outcome
- GFP (Green fluorescent protein) expressione0
- Direction
- recoverede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of NbAGO2 by transient expression of an hpRNA construct recovered GFP (Green fluorescent protein) expression in GFP-silenced plant, demonstrating that NbAGO2 participated in RNAi process in N. benthamiana.”
Claim · model-written summary
MeSA treatment induced NbAGO2 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- MeSA (Methylsalicylate acid) treatmente0
- Gene
- NbAGO2e0
- Outcome
- Expression of NbAGO2e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of NbAGO2 was transcriptionally induced by both MeSA (Methylsalicylate acid) treatment and TMV infection.”
t0“Nicotiana benthamiana”
Claim · model-written summary
TMV infection induced NbAGO2 expression.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- TMV infectione0
- Interactor
- TMV
Tobacco mosaic viruse0pathogen - Gene
- NbAGO2e0
- Outcome
- Expression of NbAGO2e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of NbAGO2 was transcriptionally induced by both MeSA (Methylsalicylate acid) treatment and TMV infection.”
t0“Nicotiana benthamiana”
Claim · model-written summary
NbAGO2 downregulation compromised resistance to TMV infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Down-regulation of NbAGO2 gene by amiR-NbAGO2 transient expressione0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- NbAGO2e0
- Outcome
- plant resistance against TMV infectione0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Down-regulation of NbAGO2 gene by amiR-NbAGO2 transient expression compromised plant resistance against TMV infection.”
t0“Nicotiana benthamiana”
Claim · model-written summary
Inhibiting miR403a reduced TMV infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- Inhibition of endogenous miR403ae0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- miR403ae0
- Outcome
- TMV infectione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of endogenous miR403a, a predicted regulatory microRNA of NbAGO2, reduced TMV infection.”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors infer SA mediates NbAGO2 antiviral defense by inducing its expression following TMV infection.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- NbAGO2e0
- Compound
- SA (Salicylic acid)e0
- Outcome
- induction of NbAGO2 expression upon TMV infectione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SA (Salicylic acid) mediates this by induction of NbAGO2 expression upon TMV infection.”
t0“Nicotiana benthamiana”
Claim · model-written summary
The authors infer miR403a regulates NbAGO2 in TMV defense.
- Taxon
- N. Benthamianae0
- Interactor
- TMVTobacco mosaic viruse0pathogen
- Gene
- miR403ae0
- Outcome
- TMV defensee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“miR403a was involved in TMV defense by regulation of target NbAGO2 gene in N. Benthamiana.”
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