Abstract
Carbon reserves in rice straw before flowering contribute greatly to grain filling. Moderate soil drying imposed at the post-anthesis stage significantly promotes carbon reserve remobilization in straws of rice, but the regulation of this process at the proteomic and transcriptomic level remains poorly understood. In this study, we applied moderate soil drying (MD) to rice at the post-anthesis stage, which was followed by dynamic proteomic and transcriptomic studies using SWATH-MS and RNA-seq analysis. MD treatment upregulated the proteins alpha-glucosidase, beta-glucosidase and starch phosphorylase, which are responsible for starch degradation. Furthermore, MD treatment enhanced the expression of proteins involved in the sucrose synthesis pathway, including SPS8 and SPP1. In addition, various monosaccharide transporters (MSTs) and sucrose transporter 2 (SUT2), which are pivotal in carbon reserve remobilization, were also upregulated in straw by MD treatment. Differentially expressed transcription factors, including GRAS, TCP, trihelix, TALE, C3H, and NF-YC, were predicted to interact with other proteins to mediate carbon reserve remobilization in response to MD treatment. Further correlation analysis revealed that the abundances of most of the differentially expressed proteins were not correlated with the corresponding transcript levels, indicating that the carbon reserve remobilization process was probably regulated by posttranscriptional modification. Our results provide insights into the molecular mechanisms underlying the regulation of carbon reserve remobilization from straw to grain in rice under MD conditions.
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Claims 6
Claim · model-written summary
Moderate soil drying upregulated starch-degradation proteins.
- Taxon
- rice
Oryza sativat0 - Treatment
- MD treatmente0
- Gene
- alpha-glucosidase, beta-glucosidase and starch phosphorylasee0
- Outcome
- proteins alpha-glucosidase, beta-glucosidase and starch phosphorylasee0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MD treatment upregulated the proteins alpha-glucosidase, beta-glucosidase and starch phosphorylase, which are responsible for starch degradation”
t0“rice straw”
Claim · model-written summary
Moderate soil drying increased SPS8 and SPP1 protein expression.
- Taxon
- riceOryza sativat0
- Treatment
- MD treatmente0
- Gene
- SPS8 and SPP1e0
- Outcome
- expression of proteins involved in the sucrose synthesis pathwaye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MD treatment enhanced the expression of proteins involved in the sucrose synthesis pathway, including SPS8 and SPP1”
t0“rice straw”
Claim · model-written summary
Moderate drying upregulated MSTs and SUT2 in straw.
- Taxon
- riceOryza sativat0
- Organ / stage
- strawe0
- Treatment
- MD treatmente0
- Outcome
- monosaccharide transporters (MSTs) and sucrose transporter 2 (SUT2)e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“various monosaccharide transporters (MSTs) and sucrose transporter 2 (SUT2), which are pivotal in carbon reserve remobilization, were also upregulated in straw by MD treatment”
t0“rice straw”
Claim · model-written summary
Differential transcription factors were predicted to interact with proteins in reserve remobilization.
- Taxon
- riceOryza sativat0
- Treatment
- MD treatmente0
- Gene
- GRAS, TCP, trihelix, TALE, C3H, and NF-YCe0
- Outcome
- carbon reserve remobilizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Differentially expressed transcription factors, including GRAS, TCP, trihelix, TALE, C3H, and NF-YC, were predicted to interact with other proteins to mediate carbon reserve remobilization in response to MD treatment”
t0“rice straw”
Claim · model-written summary
Most differential protein abundances did not correlate with corresponding transcripts.
- Taxon
- riceOryza sativat0
- Gene
- differentially expressed proteinse0
- Outcome
- abundances of most of the differentially expressed proteinse0
- Direction
- not correlatede0
- Qualifier
- corresponding transcript levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the abundances of most of the differentially expressed proteins were not correlated with the corresponding transcript levels”
t0“rice straw”
Claim · model-written summary
Authors infer probable posttranscriptional regulation of reserve remobilization.
- Taxon
- riceOryza sativat0
- Outcome
- carbon reserve remobilization processe0
- Qualifier
- posttranscriptional modificatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“carbon reserve remobilization process was probably regulated by posttranscriptional modification”
t0“rice straw”
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