Abstract
Gibberellins (GAs) regulate diverse aspects of growth and development, but their role in root development and lateral root (LR) formation is poorly understood. In this study, GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment. Microscopic examination showed that GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root. Interestingly, exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period. Moreover, the content of GAs, auxin and abscisic acid in root was altered. RNA-seq results revealed that application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes. The changes in expression of gene concerning other hormones were also detected. In addition, GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology. In response to increased GA3, 103 transcription factors were detected. Thus, exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes.
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Claims 11
Claim · model-written summary
GA3 seed soaking significantly inhibited early soybean root length and surface area.
- Taxon
- soybean
Glycine maxe0 - Treatment
- GA3 treatmente0
- Outcome
- root length and root surface areae0
- Direction
- inhibitede0
- Qualifier
- early stagese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA3 was applied to soybean [Glycine max (L.) Merr] by seed soaking. The results showed that root length and root surface area were significantly inhibited in early stages after GA3 treatment.”
Claim · model-written summary
GA3 changed cortex thickness, pericycle diameter and main-root cell size.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- GA3 treatmente0
- Outcome
- cortex thickness, the pericycle diameter, and cell size in main roote0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA3 treatment changed the cortex thickness, the pericycle diameter, and cell size in main root.”
t0“Glycine max”
Claim · model-written summary
Exogenous GA3 increased lateral root primordia number.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- exogenous GA3e0
- Outcome
- quantity of lateral root primordiae0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA3 increased the quantity of lateral root primordia (LRP)”
t0“Glycine max”
Claim · model-written summary
GA3 decreased lateral root number during the same period.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- exogenous GA3e0
- Outcome
- LR numbere0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA3 increased the quantity of lateral root primordia (LRP), but LR number decreased in this period.”
t0“Glycine max”
Claim · model-written summary
GA3 altered root contents of gibberellins, auxin and abscisic acid.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- gibberellinst0
- Outcome
- content of GAs, auxin and abscisic acid in roote0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the content of GAs, auxin and abscisic acid in root was altered”
t0“Glycine max seeds with gibberellins”
Claim · model-written summary
GA3 changed expression of GA biosynthesis genes GA20ox and GA2ox.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- application of GA3e0
- Gene
- GA20ox and GA2oxe0
- Outcome
- expression of genes in GA biosynthesis pathwaye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox”
t0“Glycine max”
Claim · model-written summary
GA3 changed expression of GA regulation and signaling genes.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- application of GA3e0
- Gene
- GA regulation genes and signalling pathway genese0
- Outcome
- expression of genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“application of GA3 not only changed the expression of genes in GA biosynthesis pathway, including GA20ox and GA2ox, but also the GA regulation genes and signalling pathway genes.”
t0“Glycine max”
Claim · model-written summary
Expression changes were also detected in genes concerning other hormones.
- Taxon
- Glycine maxGlycine maxt0
- Gene
- gene concerning other hormonese0
- Outcome
- changes in expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The changes in expression of gene concerning other hormones were also detected.”
t0“Glycine max”
Claim · model-written summary
GA3 altered cell-wall biogenesis and degradation genes.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- GA3e0
- Gene
- cell wall biogenesis and degradation genese0
- Outcome
- cell wall biogenesis and degradation genese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GA3 altered cell wall biogenesis and degradation genes which might be related to the changes of root morphology.”
t0“Glycine max”
Claim · model-written summary
Researchers detected 103 transcription factors in response to increased GA3.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- increased GA3e0
- Outcome
- transcription factorse0
- Value
- 103 transcription factorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In response to increased GA3, 103 transcription factors were detected.”
t0“Glycine max”
Claim · model-written summary
The authors propose that GA3 affects root development through changed expression of related genes.
- Taxon
- Glycine maxGlycine maxt0
- Treatment
- exogenous GA3e0
- Outcome
- root developmente0
- Qualifier
- regulating the expression of respective genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous GA3 changed the content of hormones in roots and affected the root development by regulating the expression of respective genes.”
t0“Glycine max”
The same questions in other papers
Gibberellic acid · growth · Glycine max (soybean)Results differ
29 papers in the same direction as this paper · 5 in the opposite direction
- no effect
- no effect
- no effect
31 papers more in this group
See all 35 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.