Abstract

Increasing zinc (Zn) concentration in wheat grain is an important global challenge due to high incidence of Zn deficiency in human populations. In this study, a two-year field experiment was conducted to investigate the effects of foliar ZnSO4 combined with various biostimulants (fulvic acid (FA), seaweed extract (SE), amino acids (AA), and microbial incubates (MI)) on Zn concentration and bioavailability in wheat grain under different soil nitrogen (N) levels (0, 120, and 240 kg N/ha). Grain Zn concentration and bioavailability were significantly enhanced by foliar Zn plus various biostimulants and soil N supply. Compared to foliar Zn alone, foliar Zn + FA resulted in 16% increase in grain Zn, mainly from insoluble Zn increases, while foliar Zn + AA caused 11% increase in grain Zn, mainly from soluble (at N0) and insoluble Zn increases (at N120). Foliar Zn + FA and Zn + AA generally resulted in higher Zn bioavailability than foliar Zn alone. Additionally, N concentration and Fe concentration and bioavailability in grain were enhanced with foliar Zn + AA and soil N application. Thus, foliar ZnSO4 plus FA and AA under optimal soil N rate (120 kg N/ha) can be an effective and economically friendly approach for achieving agronomic biofortification.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

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Claims 11

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Foliar zinc plus biostimulants and soil nitrogen significantly increased grain zinc concentration and bioavailability.

Treatment
foliar Zn plus various biostimulants and soil N supplye0
Outcome
Grain Zn concentration and bioavailabilitye0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Grain Zn concentration and bioavailability were significantly enhanced by foliar Zn plus various biostimulants and soil N supply”

✓ located verbatim in the source · abstract, characters 492–620 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar zinc plus fulvic acid increased grain zinc by 16% over zinc alone.

Treatment
foliar Zn + FAe0
Outcome
grain Zne0
Direction
increasee0
Value
16%e0
Comparator
foliar Zn alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Compared to foliar Zn alone, foliar Zn + FA resulted in 16% increase in grain Zn”

✓ located verbatim in the source · abstract, characters 622–702 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar zinc plus amino acids increased grain zinc by 11% over zinc alone.

Treatment
foliar Zn + AAe0
Outcome
grain Zne0
Direction
increasee0
Value
11%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“foliar Zn + AA caused 11% increase in grain Zn”

✓ located verbatim in the source · abstract, characters 746–792 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The fulvic acid combination increased insoluble grain zinc.

Treatment
foliar Zn + FAe0
Outcome
insoluble Zne0
Direction
increasese0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“foliar Zn + FA resulted in 16% increase in grain Zn, mainly from insoluble Zn increases”

✓ located verbatim in the source · abstract, characters 651–738 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The amino acid combination increased soluble zinc at N0.

Treatment
foliar Zn + AAe0
Outcome
solublee0
Direction
increasee0
Qualifier
at N0e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“foliar Zn + AA caused 11% increase in grain Zn, mainly from soluble (at N0) and insoluble Zn increases (at N120)”

✓ located verbatim in the source · abstract, characters 746–858 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The amino acid combination increased insoluble zinc at N120.

Treatment
foliar Zn + AAe0
Outcome
insoluble Zne0
Direction
increasese0
Qualifier
at N120e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“foliar Zn + AA caused 11% increase in grain Zn, mainly from soluble (at N0) and insoluble Zn increases (at N120)”

✓ located verbatim in the source · abstract, characters 746–858 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar zinc plus fulvic acid generally improved zinc bioavailability relative to zinc alone.

Treatment
Foliar Zn + FAe0
Outcome
Zn bioavailabilitye0
Direction
highere0
Comparator
foliar Zn alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Foliar Zn + FA and Zn + AA generally resulted in higher Zn bioavailability than foliar Zn alone”

✓ located verbatim in the source · abstract, characters 860–955 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar zinc plus amino acids generally improved zinc bioavailability relative to zinc alone.

Treatment
Zn + AAe0
Outcome
Zn bioavailabilitye0
Direction
highere0
Comparator
foliar Zn alonee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Foliar Zn + FA and Zn + AA generally resulted in higher Zn bioavailability than foliar Zn alone”

✓ located verbatim in the source · abstract, characters 860–955 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar zinc plus amino acids and soil nitrogen increased grain nitrogen concentration.

Treatment
foliar Zn + AA and soil N applicatione0
Outcome
N concentratione0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“N concentration and Fe concentration and bioavailability in grain were enhanced with foliar Zn + AA and soil N application”

✓ located verbatim in the source · abstract, characters 971–1093 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar zinc plus amino acids and soil nitrogen increased grain iron concentration and bioavailability.

Treatment
foliar Zn + AA and soil N applicatione0
Outcome
Fe concentration and bioavailability in graine0
Direction
enhancede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“N concentration and Fe concentration and bioavailability in grain were enhanced with foliar Zn + AA and soil N application”

✓ located verbatim in the source · abstract, characters 971–1093 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors recommend zinc sulfate plus fulvic acid and amino acids at 120 kg N/ha for biofortification.

Outcome
foliar ZnSO4 plus FA and AA under optimal soil N rate (120 kg N/ha)e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“foliar ZnSO4 plus FA and AA under optimal soil N rate (120 kg N/ha) can be an effective and economically friendly approach for achieving agronomic biofortification”

✓ located verbatim in the source · abstract, characters 1101–1264 · show in text ↑
t0

“The Effectiveness of Foliar Applications of Zinc and Biostimulants to Increase Zinc Concentration and Bioavailability of Wheat Grain”

✓ located verbatim in the source · title, characters 0–132 · show in text ↑

zinc atom · nutrient: content · Triticum aestivum (wheat)Results differ

380 papers: 347 positive, 33 negative or no effect

346 papers in the same direction as this paper · 33 in the opposite direction

  1. no effect
  2. positive
  3. positive

376 papers more in this group

See all 380 papers →

other · nutrient: content · Triticum aestivum (wheat)Positive and negative results

75 papers: 64 positive, 11 negative or no effect

63 papers in the same direction as this paper · 11 in the opposite direction

  1. positive
  2. no effect
  3. no effect

71 papers more in this group

See all 75 papers →

application · nutrient: content · Triticum aestivum (wheat)Positive and negative results

275 papers: 257 positive, 18 negative or no effect

256 papers in the same direction as this paper · 17 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. positive
  3. positive

271 papers more in this group

See all 275 papers →

nitrogen · nutrient: content · Triticum aestivum (wheat)Positive and negative results

479 papers: 410 positive, 69 negative or no effect

409 papers in the same direction as this paper · 64 in the opposite direction · 5 reporting no difference from an alternative

  1. negative
  2. positive
  3. no effect

475 papers more in this group

See all 479 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.