Abstract
Spermidine possesses multiple healthy functions, and soybeans contain the most abundant spermidine. In this study, spermidine contents of soybeans from different varieties and production regions in China were evaluated, and a spermidine synthase gene (speE) was identified by recombinant expression, transcriptional verification, and sequence analysis. Spermidine contents of soybean samples from 18 varieties ranged 72.38–228.82 mg/kg, and those from 19 production regions ranged 134.64–242.32 mg/kg. The highest-spermidine sample GZ was used to clone four predicted speE genes. Expressing the gene speE5 improved the spermidine titer by 54% in Bacillus amyloliquefaciens, confirming that speE5 was involved in spermidine synthesis. Transcriptional verification was performed through a soybean germination model. Germination for 48 h led to a onefold increase of spermidine in samples SHX and HB, and corresponding speE5 transcriptional levels were improved by 26-fold and 18-fold, respectively, further verifying the function of speE5. Finally, the sequences of the speE5 gene and deduced amino acids were analyzed, and the conserved sites and catalysis mechanisms were presented. This study identified an active spermidine synthase gene from soybean for the first time, which provided an important gene resource for genetic breeding of spermidine-rich soybean or microbial cell factory.
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Claims 7
Claim · model-written summary
Soybean varieties contained 72.38–228.82 mg/kg spermidine.
- Taxon
- soybean
Glycine maxe0 - Outcome
- Spermidine contentse0
- Value
- 72.38–228.82 mg/kge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Spermidine contents of soybean samples from 18 varieties ranged 72.38–228.82 mg/kg”
Claim · model-written summary
Soybean samples from production regions contained 134.64–242.32 mg/kg spermidine.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- production regionse0
- Value
- 134.64–242.32 mg/kge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those from 19 production regions ranged 134.64–242.32 mg/kg”
t0“Soybean”
Claim · model-written summary
Expression of soybean speE5 increased spermidine titer in Bacillus amyloliquefaciens by 54%.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Expressing the gene speE5e0
- Gene
- speE5e0
- Outcome
- spermidine titere0
- Direction
- improvede0
- Value
- 54%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“Expressing the gene speE5 improved the spermidine titer by 54% in Bacillus amyloliquefaciens”
Claim · model-written summary
Germination for 48 hours doubled spermidine in SHX and HB soybean samples.
- Taxon
- SoybeanGlycine maxt0
- Material
- SHX and HBe0
- Treatment
- Germination for 48 he0
- Outcome
- spermidinee0
- Direction
- increasee0
- Value
- onefolde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“Germination for 48 h led to a onefold increase of spermidine in samples SHX and HB”
Claim · model-written summary
Germination increased speE5 transcription 26-fold in SHX.
- Taxon
- SoybeanGlycine maxt0
- Material
- SHXe0
- Gene
- speE5e0
- Outcome
- speE5 transcriptional levelse0
- Direction
- improvede0
- Value
- 26-folde0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“samples SHX and HB, and corresponding speE5 transcriptional levels were improved by 26-fold and 18-fold, respectively”
Claim · model-written summary
Germination increased speE5 transcription 18-fold in HB.
- Taxon
- SoybeanGlycine maxt0
- Material
- HBe0
- Gene
- speE5e0
- Outcome
- speE5 transcriptional levelse0
- Direction
- improvede0
- Value
- 18-folde0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“samples SHX and HB, and corresponding speE5 transcriptional levels were improved by 26-fold and 18-fold, respectively”
Claim · model-written summary
The study identified an active soybean spermidine synthase gene.
- Taxon
- soybeanGlycine maxe0
- Gene
- speEe1
- Outcome
- active spermidine synthase genee0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study identified an active spermidine synthase gene from soybean for the first time”
e1“a spermidine synthase gene (speE) was identified”
The same questions in other papers
processing · molecular: metabolites · Glycine max (soybean)Positive and negative results
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- positive
- negative
- negative
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