Abstract
Cytokinins control critical aspects of plant development and environmental responses. Perception of cytokinin ultimately leads to the activation of proteins belonging to the type-B Response Regulator family of cytokinin response activators. In Arabidopsis thaliana, ARR1 is one of the most abundantly expressed type-B Response Regulators. We investigated the link between cytokinin signaling, protein synthesis, plant growth and osmotic stress tolerance. We show that the increased cytokinin signaling in ARR1 gain-of-function transgenic lines is associated with increased rates of protein synthesis, which lead to growth inhibition and hypersensitivity to osmotic stress. Cytokinin-induced growth inhibition and osmotic stress hypersensitivity were rescued by treatments with ABA, a hormone known to inhibit protein synthesis. We also demonstrate that cytokinin-induced protein synthesis requires isoforms of the ribosomal protein L4 encoded by the cytokinin-inducible genes RPL4A and RPL4D, and that RPL4 loss-of-function increases osmotic stress tolerance and decreases sensitivity to cytokinin-induced growth inhibition. These findings reveal that an increase in protein synthesis negatively impacts growth and osmotic stress tolerance and explain some of the adverse effects of elevated cytokinin action on plant development and stress physiology.
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Claims 8
Claim · model-written summary
ARR1 gain of function lines with increased cytokinin signaling had increased protein synthesis rates.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Material
- ARR1 gain-of-function transgenic linese0
- Gene
- ARR1e0
- Outcome
- rates of protein synthesise0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, ARR1 is one of the most abundantly expressed type-B Response Regulators. We investigated the link between cytokinin signaling, protein synthesis, plant growth and osmotic stress tolerance. We show that the increased cytokinin signaling in ARR1 gain-of-function transgenic lines is associated with increased rates of protein synthesis”
Claim · model-written summary
Increased cytokinin signaling and protein synthesis in ARR1 gain of function lines led to growth inhibition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Material
- ARR1 gain-of-function transgenic linese0
- Gene
- ARR1e0
- Outcome
- growth inhibitione0
- Qualifier
- increased rates of protein synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, ARR1 is one of the most abundantly expressed type-B Response Regulators. We investigated the link between cytokinin signaling, protein synthesis, plant growth and osmotic stress tolerance. We show that the increased cytokinin signaling in ARR1 gain-of-function transgenic lines is associated with increased rates of protein synthesis, which lead to growth inhibition”
Claim · model-written summary
Increased protein synthesis in ARR1 gain of function lines led to osmotic stress hypersensitivity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- ARR1e0
- Outcome
- hypersensitivity to osmotic stresse0
- Qualifier
- increased rates of protein synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, ARR1 is one of the most abundantly expressed type-B Response Regulators. We investigated the link between cytokinin signaling, protein synthesis, plant growth and osmotic stress tolerance. We show that the increased cytokinin signaling in ARR1 gain-of-function transgenic lines is associated with increased rates of protein synthesis, which lead to growth inhibition and hypersensitivity to osmotic stress”
Claim · model-written summary
ABA treatment rescued cytokinin induced growth inhibition.
Not reportedTaxonComparator
Verbatim
e0“Cytokinin-induced growth inhibition and osmotic stress hypersensitivity were rescued by treatments with ABA”
t0“Cytokinin‐induced protein synthesis suppresses growth and osmotic stress tolerance”
Claim · model-written summary
ABA treatment rescued cytokinin induced osmotic stress hypersensitivity.
Not reportedTaxonComparator
Verbatim
e0“Cytokinin-induced growth inhibition and osmotic stress hypersensitivity were rescued by treatments with ABA”
Claim · model-written summary
Cytokinin induced protein synthesis required RPL4A and RPL4D encoded ribosomal protein L4 isoforms.
Not reportedTaxon
Verbatim
e0“cytokinin-induced protein synthesis requires isoforms of the ribosomal protein L4 encoded by the cytokinin-inducible genes RPL4A and RPL4D”
Claim · model-written summary
RPL4 loss of function increased osmotic stress tolerance.
Not reportedTaxon
Verbatim
e0“RPL4 loss-of-function increases osmotic stress tolerance”
Claim · model-written summary
RPL4 loss of function decreased sensitivity to cytokinin induced growth inhibition.
Not reportedTaxon
Verbatim
e0“RPL4 loss-of-function increases osmotic stress tolerance and decreases sensitivity to cytokinin-induced growth inhibition”
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