Abstract
MAIN CONCLUSION: The accumulation of NiCo following the termination of the accumulation of iron in chloroplast suggests that NiCo is not solely involved in iron uptake processes of chloroplasts. Chloroplast iron (Fe) uptake is thought to be operated by a complex containing permease in chloroplast 1 (PIC1) and nickel-cobalt transporter (NiCo) proteins, whereas the role of other Fe homeostasis-related transporters such as multiple antibiotic resistance protein 1 (MAR1) is less characterized. Although pieces of information exist on the regulation of chloroplast Fe uptake, including the effect of plant Fe homeostasis, the whole system has not been revealed in detail yet. Thus, we aimed to follow leaf development-scale changes in the chloroplast Fe uptake components PIC1, NiCo and MAR1 under deficient, optimal and supraoptimal Fe nutrition using Brassica napus as model. Fe deficiency decreased both the photosynthetic activity and the Fe content of plastids. Supraoptimal Fe nutrition caused neither Fe accumulation in chloroplasts nor any toxic effects, thus only fully saturated the need for Fe in the leaves. In parallel with the increasing Fe supply of plants and ageing of the leaves, the expression of BnPIC1 was tendentiously repressed. Though transcript and protein amount of BnNiCo tendentiously increased during leaf development, it was even markedly upregulated in ageing leaves. The relative transcript amount of BnMAR1 increased mainly in ageing leaves facing Fe deficiency. Taken together chloroplast physiology, Fe content and transcript amount data, the exclusive participation of NiCo in the chloroplast Fe uptake is not supported. Saturation of the Fe requirement of chloroplasts seems to be linked to the delay of decomposing the photosynthetic apparatus and keeping chloroplast Fe homeostasis in a rather constant status together with a supressed Fe uptake machinery.
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Claims 10
Claim · model-written summary
Iron deficiency reduced photosynthetic activity.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Treatment
- Fe deficiencye0
- Outcome
- photosynthetic activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Fe deficiency decreased both the photosynthetic activity and the Fe content of plastids.”
t0“Brassica napus”
Claim · model-written summary
Iron deficiency reduced plastid iron content.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- Fe deficiencye0
- Outcome
- Fe content of plastidse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fe deficiency decreased both the photosynthetic activity and the Fe content of plastids.”
t0“Brassica napus”
Claim · model-written summary
Supraoptimal iron did not increase chloroplast iron.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- Supraoptimal Fe nutritione0
- Outcome
- Fe accumulation in chloroplastse0
- Direction
- neithere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Supraoptimal Fe nutrition caused neither Fe accumulation in chloroplasts nor any toxic effects”
t0“Brassica napus”
Claim · model-written summary
Supraoptimal iron caused no toxic effects.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- Supraoptimal Fe nutritione0
- Outcome
- toxic effectse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Supraoptimal Fe nutrition caused neither Fe accumulation in chloroplasts nor any toxic effects”
t0“Brassica napus”
Claim · model-written summary
Increasing iron supply and leaf age tended to repress BnPIC1 expression.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- increasing Fe supplye0
- Gene
- BnPIC1e0
- Outcome
- expression of BnPIC1e0
- Direction
- repressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In parallel with the increasing Fe supply of plants and ageing of the leaves, the expression of BnPIC1 was tendentiously repressed.”
t0“Brassica napus”
Claim · model-written summary
BnNiCo transcript and protein amounts tended to increase during leaf development.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnNiCoe0
- Outcome
- transcript and protein amount of BnNiCoe0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript and protein amount of BnNiCo tendentiously increased during leaf development”
t0“Brassica napus”
Claim · model-written summary
BnNiCo was markedly upregulated in ageing leaves.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- ageing leavese0
- Outcome
- BnNiCoe1
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“it was even markedly upregulated in ageing leaves.”
t0“Brassica napus”
e1“transcript and protein amount of BnNiCo tendentiously increased during leaf development”
Claim · model-written summary
BnMAR1 transcripts increased mainly in ageing iron-deficient leaves.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- ageing leavese0
- Gene
- BnMAR1e0
- Outcome
- relative transcript amount of BnMAR1e0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The relative transcript amount of BnMAR1 increased mainly in ageing leaves facing Fe deficiency.”
t0“Brassica napus”
Claim · model-written summary
The data do not support exclusive participation of NiCo in chloroplast iron uptake.
- Taxon
- Brassica napusBrassica napust0
- Gene
- NiCoe0
- Outcome
- chloroplast Fe uptakee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the exclusive participation of NiCo in the chloroplast Fe uptake is not supported.”
t0“Brassica napus”
Claim · model-written summary
The authors suggest iron requirement saturation delays photosynthetic apparatus breakdown and maintains chloroplast iron homeostasis.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- chloroplast Fe homeostasise0
- Qualifier
- Saturation of the Fe requirement of chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Saturation of the Fe requirement of chloroplasts seems to be linked to the delay of decomposing the photosynthetic apparatus and keeping chloroplast Fe homeostasis in a rather constant status together with a supressed Fe uptake machinery.”
t0“Brassica napus”
The same questions in other papers
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1 paper in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- positive
iron atom · nutrient: content · Brassica napus (oilseed rape)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.