Abstract
Objective: This study focused on the genotoxic efficacy of higher concentrations of methanolic extract of Cissus latifolia Lam. and its genoprotective role at lower concentrations against the induced cytogenetic aberrations by 2% hydrogen peroxide [H2O2] on the Allium cepa plant model. Methods: Cytotoxic and genoprotective potential of the plant extract were analyzed using A. cepa assay. The treatment modes that were designed for antigenotoxicity screening were pre-treatment, post-treatment, and concurrent treatment. Results: Results revealed the toxic effect of extract of C. latifolia which induced major chromosomal abnormalities, namely, nuclear lesions, chromosome vagrants, stickiness, bridges, and chromosome breaks. These aberrations were found to be unaltered and showed the potent clastogenic nature of plant extract. As concentrations of plant extract and exposure time increase, the frequency of occurrence of cytogenetic aberrations was also detected to be more. Further, the potential genotoxicity of plant measured in terms of mitotic index reduction also confirms its significant role in inducing mutagenicity. The genoprotective efficacy of extract was identified as its ability to inhibit and reverse the aberrations caused by H2O2 at lower concentrations of plant extract. Conclusions: Dose-dependent data highlight the cytotoxic and genoprotective effect of C. latifolia on the A. cepa model. These findings will help to exploit the plant as an anticancer drug and explore other wide arrays of therapeutic activities of C. latifolia.
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Claims 6
Claim · model-written summary
Cissus latifolia extract induced nuclear lesions, chromosome vagrants and stickiness in Allium cepa.
- Taxon
- Allium cepa
Allium cepae1 - Treatment
- extract of C. latifoliae2
- Outcome
- nuclear lesions, chromosome vagrants, stickinesse2
- Direction
- inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Allium cepa plant model”
e2“Results revealed the toxic effect of extract of C. latifolia which induced major chromosomal abnormalities, namely, nuclear lesions, chromosome vagrants, stickiness, bridges, and chromosome breaks”
Claim · model-written summary
Cissus latifolia extract induced bridges and chromosome breaks in Allium cepa.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- extract of C. latifoliae2
- Outcome
- bridges, and chromosome breakse2
- Direction
- inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Allium cepa plant model”
e2“Results revealed the toxic effect of extract of C. latifolia which induced major chromosomal abnormalities, namely, nuclear lesions, chromosome vagrants, stickiness, bridges, and chromosome breaks”
Claim · model-written summary
Cissus latifolia extract showed a potent clastogenic nature in Allium cepa.
- Taxon
- Allium cepaAllium cepae1
- Outcome
- clastogenic nature of plant extracte3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Allium cepa plant model”
e3“showed the potent clastogenic nature of plant extract”
Claim · model-written summary
Frequency of cytogenetic aberrations in Allium cepa increased with extract concentration and exposure time.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- concentrations of plant extract and exposure timee4
- Outcome
- frequency of occurrence of cytogenetic aberrationse4
- Direction
- moree4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Allium cepa plant model”
e4“As concentrations of plant extract and exposure time increase, the frequency of occurrence of cytogenetic aberrations was also detected to be more”
Claim · model-written summary
Mitotic index was reduced by the plant extract in Allium cepa, indicating significant mutagenic potential.
- Taxon
- Allium cepaAllium cepae1
- Outcome
- mitotic indexe5
- Direction
- reductione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Allium cepa plant model”
e5“the potential genotoxicity of plant measured in terms of mitotic index reduction also confirms its significant role in inducing mutagenicity”
Claim · model-written summary
Lower concentrations of the extract inhibited and reversed H2O2-induced aberrations in Allium cepa.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- lower concentrations of plant extracte6
- Compound
- H2O2e6
- Outcome
- the aberrations caused by H2O2e6
- Direction
- inhibit and reversee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Allium cepa plant model”
e6“The genoprotective efficacy of extract was identified as its ability to inhibit and reverse the aberrations caused by H2O2 at lower concentrations of plant extract”
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