Paper · 2020 · Australian Journal of Crop Science

Osmoprotectant in soybean seeds can increase the inoculation and co-inoculation time in pre-sowing

Glauber Monçon Fipke; Thomas Newton Martin; Tânia Maria Müller; et al.

6
claims
16
verbatim quotes
7
citations
3
research areas
✓
Open access

Abstract

The practice of soybean seed inoculation cannot exceed the 24-hour prior to sowing. The objective of this study was to assess the effects of inoculation and co-inoculation of osmoprotectant soybean seeds, performed at different times of sowing. The experiments were conducted during two crop seasons at "sandy clay loam Acrisol" and "Cfa climate" in Santa Maria, RS, Brazil. The experimental design consisted of completely randomized blocks with four replications. Nine distinct treatments of seeds inoculation were carried out with bacteria of the genus Bradyrhizobium were inoculated alone (conventional inoculation) or combined with Azospirillum brasilense (co-inoculation), associated with the use of osmoprotectants. The components of nodulation, leaf chlorophyll, plant growth and grain yield were evaluated. The number of nodules and pods per plant are the variables most correlated with grain yield. Number of grains per pod, mass of thousand grains, and A, B and total chlorophyll content were not influenced by inoculation, co-inoculation and nitrogen fertilization. All treatments that used osmoprotectant had an increased 30% (in the first year) at average number of nodules in V5 compared to the treatments that did not use it. Co-inoculation provided an increase of 31% and 16% of yield, respectively, compared to the no-inoculation treatment, for the first and second experimental years. Inoculation and co-inoculation when carried out seven days before sowing and associated with the use of the osmoprotector, provides grain yield similar to the inoculation performed at the time of sowing.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Osmoprotectant treatments increased the average number of nodules at V5 by 30% in the first year.

Taxon
soybeanGlycine maxt0
Treatment
osmoprotectante0
Interactor
BradyrhizobiumBradyrhizobiume1symbiont
Outcome
average number of nodulese0
Direction
increasede0
Value
30%e0
Qualifier
in V5e0
Comparator
the treatments that did not use ite0
Setting
first yeare0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Bradyrhizobium
Bradyrhizobium · Interactor · picture of the genus (one of its species)Louisa Howard - Dartmouth Electron Microscope Facility · Public domain · Wikimedia Commons ↗

Verbatim

t0

“soybean seeds”

✓ located verbatim in the source · title, characters 18–31 · show in text ↑
e0

“All treatments that used osmoprotectant had an increased 30% (in the first year) at average number of nodules in V5 compared to the treatments that did not use it”

✓ located verbatim in the source · abstract, characters 1077–1239 · show in text ↑
e1

“bacteria of the genus Bradyrhizobium”

✓ located verbatim in the source · abstract, characters 533–569 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation increased soybean grain yield by 31% in the first experimental year compared with no inoculation.

Taxon
soybeanGlycine maxt0
Treatment
Co-inoculatione0
Interactor
Azospirillum brasilenseAzospirillum brasilensee1symbiont
Outcome
yielde0
Direction
increasee0
Value
31%e0
Qualifier
firste0
Comparator
no-inoculation treatmente0
Setting
experimental yearse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“soybean seeds”

✓ located verbatim in the source · title, characters 18–31 · show in text ↑
e0

“Co-inoculation provided an increase of 31% and 16% of yield, respectively, compared to the no-inoculation treatment, for the first and second experimental years”

✓ located verbatim in the source · abstract, characters 1241–1401 · show in text ↑
e1

“combined with Azospirillum brasilense (co-inoculation)”

✓ located verbatim in the source · abstract, characters 622–676 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Co-inoculation increased soybean grain yield by 16% in the second experimental year compared with no inoculation.

Taxon
soybeanGlycine maxt0
Treatment
Co-inoculatione0
Interactor
Azospirillum brasilenseAzospirillum brasilensee1symbiont
Outcome
yielde0
Direction
increasee0
Value
16%e0
Qualifier
seconde0
Comparator
no-inoculation treatmente0
Setting
experimental yearse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“soybean seeds”

✓ located verbatim in the source · title, characters 18–31 · show in text ↑
e0

“Co-inoculation provided an increase of 31% and 16% of yield, respectively, compared to the no-inoculation treatment, for the first and second experimental years”

✓ located verbatim in the source · abstract, characters 1241–1401 · show in text ↑
e1

“combined with Azospirillum brasilense (co-inoculation)”

✓ located verbatim in the source · abstract, characters 622–676 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Number of nodules and pods per plant were the variables most correlated with grain yield.

Taxon
soybeanGlycine maxt0
Organ / stage
nodulese0
Outcome
number of nodules and pods per plante0
Direction
most correlatede0
Qualifier
grain yielde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“soybean seeds”

✓ located verbatim in the source · title, characters 18–31 · show in text ↑
e0

“The number of nodules and pods per plant are the variables most correlated with grain yield”

✓ located verbatim in the source · abstract, characters 815–906 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Inoculation, co-inoculation and nitrogen fertilization did not influence grains per pod, thousand-grain mass or chlorophyll content.

Taxon
soybeanGlycine maxt0
Treatment
inoculation, co-inoculation and nitrogen fertilizatione0
Outcome
Number of grains per pod, mass of thousand grains, and A, B and total chlorophyll contente0
Direction
not influencede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“soybean seeds”

✓ located verbatim in the source · title, characters 18–31 · show in text ↑
e0

“Number of grains per pod, mass of thousand grains, and A, B and total chlorophyll content were not influenced by inoculation, co-inoculation and nitrogen fertilization”

✓ located verbatim in the source · abstract, characters 908–1075 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Inoculation and co-inoculation seven days before sowing gave grain yield similar to inoculation at sowing.

Taxon
soybeanGlycine maxt0
Treatment
Inoculation and co-inoculation when carried out seven days before sowinge0
Outcome
grain yielde1
Direction
similare1
Comparator
the inoculation performed at the time of sowinge1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

t0

“soybean seeds”

✓ located verbatim in the source · title, characters 18–31 · show in text ↑
e0

“Inoculation and co-inoculation when carried out seven days before sowing”

✓ located verbatim in the source · abstract, characters 1403–1475 · show in text ↑
e1

“provides grain yield similar to the inoculation performed at the time of sowing”

✓ located verbatim in the source · abstract, characters 1526–1605 · show in text ↑

fertilization · physiology: pigments · Glycine max (soybean)Results differ

46 papers: 42 positive, 4 negative or no effect

3 papers in the same direction as this paper · 42 in the opposite direction

  1. positive
  2. positive
  3. positive

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inoculation · Azospirillum brasilense · Glycine max (soybean)Positive and negative results

11 papers: 8 positive, 3 negative or no effect

7 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. no effect
  3. no effect

7 papers more in this group

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inoculation · physiology: pigments · Glycine max (soybean)Positive and negative results

44 papers: 35 positive, 9 negative or no effect

8 papers in the same direction as this paper · 35 in the opposite direction

  1. positive
  2. positive
  3. positive

40 papers more in this group

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inoculation · yield · Glycine max (soybean)Positive and negative results

566 papers: 491 positive, 75 negative or no effect

74 papers in the same direction as this paper · 491 reporting no difference from an alternative

  1. positive
  2. positive
  3. positive

562 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.