Abstract
Thioredoxins (Trxs) function within the antioxidant network through modulation of one or more redox reactions involved in oxidative-stress signaling. Given their function in regulating cellular redox, Trx proteins also fulfill key roles in plant immune signaling. Here, TaTrxh1, encoding a subgroup h member of the Trx family, was identified and cloned in wheat (Triticum aestivum), which was rapidly induced by Puccinia striiformis f. sp. tritici invasion and salicylic acid (SA) treatment. Overexpression of TaTrxh1 in tobacco (Nicotiana benthamiana) induced programmed cell death. Silencing of TaTrxh1 in wheat enhanced susceptibility to P. striiformis f. sp. tritici in different aspects, including reactive oxygen species accumulation and pathogen-responsive or -related gene expression. Herein, we observed that the cellular concentration of SA was significantly reduced in TaTrxh1-silenced plants, indicating that TaTrxh1 possibly regulates wheat resistance to stripe rust through a SA-associated defense signaling pathway. Using a yeast two-hybrid screen to identify TaTrxh1-interacting partners, we further show that interaction with TaCP1 (a RD19-like cysteine protease) and subsequent silencing of TaCP1 reduced wheat resistance to P. striiformis f. sp. tritici. In total, the data presented herein demonstrate that TaTrxh1 enhances wheat resistance against P. striiformis f. sp. tritici via SA-dependent resistance signaling and that TaTrxh1 interaction with TaCP1 is required for wheat resistance to stripe rust. [Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
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Claims 8
Claim · model-written summary
TaTrxh1 in wheat was rapidly induced by Puccinia striiformis f. sp. tritici invasion.
- Taxon
- wheat
Triticum aestivume1 - Treatment
- Puccinia striiformis f. sp. tritici invasione1
- Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie1pathogen - Gene
- TaTrxh1e1
- Outcome
- TaTrxh1e1
- Direction
- rapidly inducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaTrxh1, encoding a subgroup h member of the Trx family, was identified and cloned in wheat (Triticum aestivum), which was rapidly induced by Puccinia striiformis f. sp. tritici invasion and salicylic acid (SA) treatment”
Claim · model-written summary
Overexpression of TaTrxh1 in tobacco induced programmed cell death.
- Taxon
- tobacco (Nicotiana benthamiana)
Nicotiana benthamianae2 - Treatment
- Overexpression of TaTrxh1e2
- Gene
- TaTrxh1e2
- Outcome
- programmed cell deathe2
- Direction
- inducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Overexpression of TaTrxh1 in tobacco (Nicotiana benthamiana) induced programmed cell death”
Claim · model-written summary
Silencing of TaTrxh1 in wheat enhanced susceptibility to Puccinia striiformis f. sp. tritici, including reactive oxygen species accumulation and pathogen-responsive gene expression.
- Taxon
- wheatTriticum aestivume3
- Treatment
- Silencing of TaTrxh1e3
- Interactor
- P. striiformis f. sp. triticiPuccinia striiformis f. sp. triticie3pathogen
- Gene
- TaTrxh1e3
- Outcome
- susceptibility to P. striiformis f. sp. triticie3
- Direction
- enhancede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Silencing of TaTrxh1 in wheat enhanced susceptibility to P. striiformis f. sp. tritici in different aspects, including reactive oxygen species accumulation and pathogen-responsive or -related gene expression”
Claim · model-written summary
The cellular concentration of SA was significantly reduced in TaTrxh1-silenced wheat plants.
- Taxon
- wheatTriticum aestivume1
- Treatment
- TaTrxh1‐silenced plantse4
- Outcome
- cellular concentration of SAe4
- Direction
- reducede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaTrxh1, encoding a subgroup h member of the Trx family, was identified and cloned in wheat (Triticum aestivum), which was rapidly induced by Puccinia striiformis f. sp. tritici invasion and salicylic acid (SA) treatment”
e4“the cellular concentration of SA was significantly reduced in TaTrxh1‐silenced plants”
Claim · model-written summary
TaTrxh1 possibly regulates wheat resistance to stripe rust through an SA-associated defense signaling pathway.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaTrxh1e5
- Outcome
- wheat resistance to stripe ruste5
- Qualifier
- SA-associated defense signaling pathwaye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaTrxh1, encoding a subgroup h member of the Trx family, was identified and cloned in wheat (Triticum aestivum), which was rapidly induced by Puccinia striiformis f. sp. tritici invasion and salicylic acid (SA) treatment”
e5“indicating that TaTrxh1 possibly regulates wheat resistance to stripe rust through a SA-associated defense signaling pathway”
Claim · model-written summary
Silencing of TaCP1 reduced wheat resistance to Puccinia striiformis f. sp. tritici.
- Taxon
- wheatTriticum aestivume1
- Treatment
- silencing of TaCP1e6
- Interactor
- P. striiformis f. sp. triticiPuccinia striiformis f. sp. triticie6pathogen
- Gene
- TaCP1e6
- Outcome
- wheat resistance to P. striiformis f. sp. triticie6
- Direction
- reducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaTrxh1, encoding a subgroup h member of the Trx family, was identified and cloned in wheat (Triticum aestivum), which was rapidly induced by Puccinia striiformis f. sp. tritici invasion and salicylic acid (SA) treatment”
e6“subsequent silencing of TaCP1 reduced wheat resistance to P. striiformis f. sp. tritici”
Claim · model-written summary
TaTrxh1 enhances wheat resistance against P. striiformis f. sp. tritici via SA-dependent resistance signaling.
- Taxon
- wheatTriticum aestivume1
- Interactor
- P. striiformis f. sp. triticiPuccinia striiformis f. sp. triticie7pathogen
- Gene
- TaTrxh1e7
- Outcome
- wheat resistance against P. striiformis f. sp. triticie7
- Qualifier
- via SA-dependent resistance signalinge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaTrxh1, encoding a subgroup h member of the Trx family, was identified and cloned in wheat (Triticum aestivum), which was rapidly induced by Puccinia striiformis f. sp. tritici invasion and salicylic acid (SA) treatment”
e7“TaTrxh1 enhances wheat resistance against P. striiformis f. sp. tritici via SA-dependent resistance signaling”
Claim · model-written summary
The interaction of TaTrxh1 with TaCP1 is required for wheat resistance to stripe rust.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaCP1e2
- Outcome
- wheat resistance to stripe ruste2
- Qualifier
- interaction with TaCP1 is requirede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“TaTrxh1, encoding a subgroup h member of the Trx family, was identified and cloned in wheat (Triticum aestivum), which was rapidly induced by Puccinia striiformis f. sp. tritici invasion and salicylic acid (SA) treatment”
e2“TaTrxh1 interaction with TaCP1 is required for wheat resistance to stripe rust”
The same questions in other papers
loss of function · Puccinia striiformis f. sp. tritici · Triticum aestivum (wheat)Positive and negative results
9 papers in the same direction as this paper · 8 in the opposite direction
- negative
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See all 18 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.