Abstract
The R2R3 transcription factor MdMYB88 has previously been reported to function in biotic and abiotic stress responses. Here, we identify BRI1 ETHYLMETHANE SULFONATE SUPRESSOR1 (MdBES1), a vital component of brassinosteroid (BR) signaling in apple (Malus × domestica) that directly binds to the MdMYB88 promoter, regulating the expression of MdMYB88 in a dynamic and multifaceted mode. MdBES1 positively regulated expression of MdMYB88 under cold stress and pathogen attack, but negatively regulated its expression under control and drought conditions. Consistently, MdBES1 was a positive regulator for cold tolerance and disease resistance in apple, but a negative regulator for drought tolerance. In addition, MdMYB88 participated in BR biosynthesis by directly regulating the BR biosynthetic genes DE ETIOLATED 2 (MdDET2), DWARF 4 (MdDWF4), and BRASSINOSTEROID 6 OXIDASE 2 (MdBR6OX2). Applying exogenous BR partially rescued the erect leaf and dwarf phenotypes, as well as defects in stress tolerance in MdMYB88/124 RNAi plants. Moreover, knockdown of MdMYB88 in MdBES1 overexpression (OE) plants decreased resistance to a pathogen and C-REPEAT BINDING FACTOR1 expression, whereas overexpressing MdMYB88 in MdBES1 OE plants increased expression of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 （MdSPL3） and BR biosynthetic genes, suggesting that MdMYB88 contributes to MdBES1 function during BR biosynthesis and the stress response. Taken together, our results reveal multifaceted regulation of MdBES1 on MdMYB88 in BR biosynthesis and stress tolerance.
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Claims 10
Claim · model-written summary
MdBES1, a component of brassinosteroid signaling in apple, directly binds to the MdMYB88 promoter.
- Taxon
- apple
Malus domesticat0 - Gene
- MdBES1e0
- Outcome
- directly binds to the MdMYB88 promotere0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“BRI1 ETHYLMETHANE SULFONATE SUPRESSOR1 (MdBES1), a vital component of brassinosteroid (BR) signaling in apple (Malus × domestica) that directly binds to the MdMYB88 promoter”
Claim · model-written summary
MdBES1 positively regulated expression of MdMYB88 under cold stress and pathogen attack.
- Taxon
- appleMalus domesticat0
- Gene
- MdBES1e0
- Outcome
- expression of MdMYB88e0
- Direction
- positively regulatede0
- Qualifier
- under cold stress and pathogen attacke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“MdBES1 positively regulated expression of MdMYB88 under cold stress and pathogen attack, but negatively regulated its expression under control and drought conditions”
Claim · model-written summary
MdBES1 negatively regulated expression of MdMYB88 under control and drought conditions.
- Taxon
- appleMalus domesticat0
- Gene
- MdBES1e0
- Outcome
- expression of MdMYB88e0
- Direction
- negatively regulatede0
- Qualifier
- under control and drought conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“MdBES1 positively regulated expression of MdMYB88 under cold stress and pathogen attack, but negatively regulated its expression under control and drought conditions”
Claim · model-written summary
MdBES1 was a positive regulator of cold tolerance and disease resistance in apple.
- Taxon
- appleMalus domesticat0
- Gene
- MdBES1e0
- Outcome
- cold tolerance and disease resistancee0
- Direction
- positive regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“MdBES1 was a positive regulator for cold tolerance and disease resistance in apple, but a negative regulator for drought tolerance”
Claim · model-written summary
MdBES1 was a negative regulator of drought tolerance in apple.
- Taxon
- appleMalus domesticat0
- Gene
- MdBES1e0
- Outcome
- drought tolerancee0
- Direction
- negative regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“MdBES1 was a positive regulator for cold tolerance and disease resistance in apple, but a negative regulator for drought tolerance”
Claim · model-written summary
MdMYB88 participated in BR biosynthesis by directly regulating the BR biosynthetic genes MdDET2, MdDWF4 and MdBR6OX2.
- Taxon
- appleMalus domesticat0
- Gene
- BR biosynthetic genes DE ETIOLATED 2 (MdDET2), DWARF 4 (MdDWF4), and BRASSINOSTEROID 6 OXIDASE 2 (MdBR6OX2)e0
- Outcome
- BR biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“MdMYB88 participated in BR biosynthesis by directly regulating the BR biosynthetic genes DE ETIOLATED 2 (MdDET2), DWARF 4 (MdDWF4), and BRASSINOSTEROID 6 OXIDASE 2 (MdBR6OX2)”
Claim · model-written summary
Exogenous BR partially rescued the erect leaf and dwarf phenotypes and the defects in stress tolerance in MdMYB88/124 RNAi plants.
- Taxon
- appleMalus domesticat0
- Material
- MdMYB88/124 RNAi plantse0
- Treatment
- exogenous BRe0
- Outcome
- erect leaf and dwarf phenotypes, as well as defects in stress tolerancee0
- Direction
- partially rescuede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“Applying exogenous BR partially rescued the erect leaf and dwarf phenotypes, as well as defects in stress tolerance in MdMYB88/124 RNAi plants”
Claim · model-written summary
Knockdown of MdMYB88 in MdBES1 overexpression plants decreased resistance to a pathogen and decreased expression of C-REPEAT BINDING FACTOR1.
- Taxon
- appleMalus domesticat0
- Material
- MdBES1 overexpression (OE) plantse0
- Treatment
- knockdown of MdMYB88e0
- Interactor
- a pathogene0pathogen
- Outcome
- resistance to a pathogen and C-REPEAT BINDING FACTOR1 expressione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“knockdown of MdMYB88 in MdBES1 overexpression (OE) plants decreased resistance to a pathogen and C-REPEAT BINDING FACTOR1 expression”
Claim · model-written summary
Overexpressing MdMYB88 in MdBES1 OE plants increased expression of MdSPL3 and BR biosynthetic genes.
- Taxon
- appleMalus domesticat0
- Material
- MdBES1 OE plantse0
- Treatment
- overexpressing MdMYB88e0
- Outcome
- expression of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 （MdSPL3） and BR biosynthetic genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“overexpressing MdMYB88 in MdBES1 OE plants increased expression of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 3 （MdSPL3） and BR biosynthetic genes”
Claim · model-written summary
The authors suggest MdMYB88 contributes to MdBES1 function during BR biosynthesis and the stress response.
- Taxon
- appleMalus domesticat0
- Gene
- MdMYB88e0
- Outcome
- MdBES1 function during BR biosynthesis and the stress responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“apple”
e0“MdMYB88 contributes to MdBES1 function during BR biosynthesis and the stress response”