Abstract
Seed dormancy and germination are key events in plant development and are critical for crop production, and defects in seed germination or the inappropriate release of seed dormancy cause substantial losses in crop yields. Rice is the staple food for more than half of the world’s population, and preharvest sprouting (PHS) is one of the most severe problems in rice production, due to a low level of seed dormancy, especially under warm and damp conditions. Therefore, PHS leads to yield loss and a decrease in rice quality and vitality. We reveal that mutation of OsbZIP09 inhibited rice PHS. Analysis of the expression of OsbZIP09 and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA. Moreover, RNA sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analyses were performed and 52 key direct targets of OsbZIP09 were identified, including OsLOX2 and Late Embryogenesis Abundant (LEA) family genes, which are involved in controlling seed germination. Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and OsbZIP09 mutation. The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.
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Claims 7
Claim · model-written summary
Mutation of OsbZIP09 inhibited rice preharvest sprouting.
- Taxon
- rice
Oryza sativae0 - Treatment
- mutation of OsbZIP09e0
- Gene
- OsbZIP09e0
- Outcome
- PHSe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We reveal that mutation of OsbZIP09 inhibited rice PHS.”
Claim · model-written summary
OsbZIP09 is a typical bZIP transcription factor containing conserved bZIP domains.
- Taxon
- RiceOryza sativat0
- Gene
- OsbZIP09e0
- Outcome
- typical bZIP transcription factor that contains conserved bZIP domainse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the expression of OsbZIP09 and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA.”
t0“Rice”
Claim · model-written summary
ABA induced OsbZIP09 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the expression of OsbZIP09 and its encoded protein sequence and structure indicated that OsbZIP09 is a typical bZIP transcription factor that contains conserved bZIP domains, and its expression is induced by ABA.”
t0“Rice”
Claim · model-written summary
Sequencing identified 52 key direct OsbZIP09 targets, including OsLOX2 and LEA family genes involved in controlling seed germination.
- Taxon
- RiceOryza sativat0
- Gene
- OsbZIP09e0
- Outcome
- key direct targetse0
- Value
- 52e0
- Qualifier
- including OsLOX2 and Late Embryogenesis Abundant (LEA) family genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“52 key direct targets of OsbZIP09 were identified, including OsLOX2 and Late Embryogenesis Abundant (LEA) family genes, which are involved in controlling seed germination.”
t0“Rice”
Claim · model-written summary
Most key OsbZIP09 targets showed consistent expression changes in response to ABA treatment.
- Taxon
- RiceOryza sativat0
- Treatment
- abscisic acid (ABA) treatmente0
- Outcome
- expressione0
- Qualifier
- Most of these key targets showed consistent changese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and OsbZIP09 mutation.”
t0“Rice”
Claim · model-written summary
Most key OsbZIP09 targets showed consistent expression changes in response to OsbZIP09 mutation.
- Taxon
- RiceOryza sativat0
- Treatment
- OsbZIP09 mutatione0
- Gene
- OsbZIP09e0
- Outcome
- expressione0
- Qualifier
- Most of these key targets showed consistent changese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of these key targets showed consistent changes in expression in response to abscisic acid (ABA) treatment and OsbZIP09 mutation.”
t0“Rice”
Claim · model-written summary
The data characterize key target genes directly regulated by OsbZIP09, informing the mechanism controlling rice seed germination.
- Taxon
- riceOryza sativae0
- Gene
- OsbZIP09e0
- Outcome
- seed germinatione0
- Qualifier
- key target genes that are directly regulated by OsbZIP09e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The data characterize a number of key target genes that are directly regulated by OsbZIP09 and contribute to revealing the molecular mechanism that underlies how OsbZIP09 controls rice seed germination.”
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.