Abstract
Water-deficit stress negatively affects wheat yield and quality. Abiotic stress on parental plants during reproduction may have transgenerational effects on progeny. Here we investigated the transgenerational influence of pre-anthesis water-deficit stress by detailed analysis of the yield components, grain quality traits, and physiological traits in durum wheat. Next-generation sequencing analysis profiled the small RNA-omics, mRNA transcriptomics, and mRNA degradomics in first generation progeny. Parental water-deficit stress had positive impacts on the progeny for traits including harvest index and protein content in the less stress-tolerant variety. Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs). Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics. Differentially expressed miRNAs and genes were identified, with significant regulatory patterns subject to trans- and inter-generational stress. Integrated analysis using three omics platforms revealed significant biological interactions between stress-responsive miRNA and targets, with transgenerational stress tolerance potentially contributed via pathways such as hormone signalling and nutrient metabolism. Our study provides the first confirmation of the transgenerational effects of water-deficit stress in durum wheat. New insights gained at the molecular level indicate that key miRNA-mRNA modules are candidates for transgenerational stress improvement.
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Claims 10
Claim · model-written summary
Parental water-deficit stress increased progeny harvest index in the less stress-tolerant variety.
- Taxon
- durum wheat
Triticum turgidum subsp. durumt0 - Treatment
- Parental water-deficit stresse0
- Outcome
- harvest indexe0
- Direction
- positive impactse0
- Qualifier
- less stress-tolerant varietye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Parental water-deficit stress had positive impacts on the progeny for traits including harvest index and protein content in the less stress-tolerant variety.”
t0“durum wheat”
Claim · model-written summary
Parental water-deficit stress increased progeny protein content in the less stress-tolerant variety.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Treatment
- Parental water-deficit stresse0
- Outcome
- protein contente0
- Direction
- positive impactse0
- Qualifier
- less stress-tolerant varietye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Parental water-deficit stress had positive impacts on the progeny for traits including harvest index and protein content in the less stress-tolerant variety.”
t0“durum wheat”
Claim · model-written summary
Small RNA sequencing identified 1739 conserved microRNAs.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Gene
- microRNAse0
- Outcome
- conservede0
- Value
- 1739e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs).”
t0“durum wheat”
Claim · model-written summary
Small RNA sequencing identified 774 novel microRNAs.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- novel microRNAse0
- Value
- 774e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Small RNA-seq identified 1739 conserved and 774 novel microRNAs (miRNAs).”
t0“durum wheat”
Claim · model-written summary
Transcriptome sequencing characterized expression of 66,559 genes.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- expressione0
- Value
- 66,559 genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics.”
t0“durum wheat”
Claim · model-written summary
Degradome sequencing profiled miRNA-guided mRNA cleavage dynamics.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- miRNA-guided mRNA cleavage dynamicse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome-seq characterised the expression of 66,559 genes while degradome-seq profiled the miRNA-guided mRNA cleavage dynamics.”
t0“durum wheat”
Claim · model-written summary
Differentially expressed miRNAs and genes showed significant regulatory patterns under trans- and inter-generational stress.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Gene
- miRNAs and genese0
- Outcome
- significant regulatory patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Differentially expressed miRNAs and genes were identified, with significant regulatory patterns subject to trans- and inter-generational stress.”
t0“durum wheat”
Claim · model-written summary
Integrated omics analysis revealed significant interactions between stress-responsive miRNAs and targets.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Gene
- stress-responsive miRNA and targetse0
- Outcome
- significant biological interactionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Integrated analysis using three omics platforms revealed significant biological interactions between stress-responsive miRNA and targets, with transgenerational stress tolerance potentially contributed via pathways such as hormone signalling and nutrient metabolism.”
t0“durum wheat”
Claim · model-written summary
Hormone signalling and nutrient metabolism pathways may contribute to transgenerational stress tolerance.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Outcome
- transgenerational stress tolerancee0
- Qualifier
- pathways such as hormone signalling and nutrient metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Integrated analysis using three omics platforms revealed significant biological interactions between stress-responsive miRNA and targets, with transgenerational stress tolerance potentially contributed via pathways such as hormone signalling and nutrient metabolism.”
t0“durum wheat”
Claim · model-written summary
Key miRNA-mRNA modules were proposed as candidates for transgenerational stress improvement.
- Taxon
- durum wheatTriticum turgidum subsp. durumt0
- Gene
- miRNA-mRNA modulese0
- Outcome
- candidates for transgenerational stress improvemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“New insights gained at the molecular level indicate that key miRNA-mRNA modules are candidates for transgenerational stress improvement.”
t0“durum wheat”
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