Abstract
Citrus sinensis lateral organ boundary 1 (CsLOB1) was previously identified as a critical disease susceptibility gene for citrus bacterial canker, which is caused by Xanthomonas citri subsp. citri (Xcc). However, the molecular mechanisms of CsLOB1 in citrus response to Xcc are still elusive. Here, we constructed transgenic plants overexpressing and RNAi-silencing of CsLOB1 using the canker-disease susceptible 'wanjincheng' orange (C. sinensis Osbeck) as explants. CsLOB1-overexpressing plants exhibited dwarf phenotypes with smaller and thicker leaf, increased branches and adventitious buds clustered on stems. These phenotypes were followed by a process of pustule- and canker-like development that exhibited enhanced cell proliferation. Pectin depolymerization and expansin accumulation were enhanced by CsLOB1 overexpression, while cellulose and hemicellulose synthesis were increased by CsLOB1 silence. Whilst overexpression of CsLOB1 increased susceptibility, RNAi-silencing of CsLOB1 enhanced resistance to canker disease without impairing pathogen entry. Transcriptome analysis revealed that CsLOB1 positively regulated cell wall degradation and modification processes, cytokinin metabolism, and cell division. Additionally, 565 CsLOB1-targeted genes were identified in chromatin immunoprecipitation-sequencing (ChIP-seq) experiments. Motif discovery analysis revealed that the most highly overrepresented binding sites had a conserved 6-bp 'GCGGCG' consensus DNA motif. RNA-seq and ChIP-seq data suggested that CsLOB1 directly activates the expression of four genes involved in cell wall remodeling, and three genes that participate in cytokinin and brassinosteroid hormone pathways. Our findings indicate that CsLOB1 promotes cell proliferation by mechanisms depending on cell wall remodeling and phytohormone signaling, which may be critical to citrus canker development and bacterial growth in citrus.
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Claims 11
Claim · model-written summary
CsLOB1 overexpression caused dwarf plants, altered leaves, and increased branches and adventitious buds.
- Treatment
- CsLOB1-overexpressinge0
- Gene
- CsLOB1e0
- Outcome
- dwarf phenotypes with smaller and thicker leaf, increased branches and adventitious buds clustered on stemse0
- Direction
- increasede0
CitrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsLOB1-overexpressing plants exhibited dwarf phenotypes with smaller and thicker leaf, increased branches and adventitious buds clustered on stems.”
t0“susceptibility to citrus canker”
Claim · model-written summary
CsLOB1 overexpression was followed by pustule-like and canker-like development with enhanced cell proliferation.
- Treatment
- CsLOB1 overexpressione1
- Outcome
- pustule- and canker-like developmente0
- Direction
- enhancede0
- Qualifier
- cell proliferatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These phenotypes were followed by a process of pustule- and canker-like development that exhibited enhanced cell proliferation.”
e1“Pectin depolymerization and expansin accumulation were enhanced by CsLOB1 overexpression”
t0“susceptibility to citrus canker”
Claim · model-written summary
CsLOB1 overexpression enhanced pectin depolymerization and expansin accumulation.
- Treatment
- CsLOB1 overexpressione0
- Gene
- CsLOB1e0
- Outcome
- Pectin depolymerization and expansin accumulatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pectin depolymerization and expansin accumulation were enhanced by CsLOB1 overexpression, while cellulose and hemicellulose synthesis were increased by CsLOB1 silence.”
t0“susceptibility to citrus canker”
Claim · model-written summary
CsLOB1 silencing increased cellulose and hemicellulose synthesis.
- Treatment
- CsLOB1 silencee0
- Gene
- CsLOB1e0
- Outcome
- cellulose and hemicellulose synthesise0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pectin depolymerization and expansin accumulation were enhanced by CsLOB1 overexpression, while cellulose and hemicellulose synthesis were increased by CsLOB1 silence.”
t0“susceptibility to citrus canker”
Claim · model-written summary
CsLOB1 overexpression increased citrus canker susceptibility.
- Treatment
- overexpression of CsLOB1e0
- Interactor
- Xanthomonas citri subsp. citri (Xcc)Xanthomonas citri pv. citrie1pathogen
- Gene
- CsLOB1e0
- Outcome
- susceptibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Whilst overexpression of CsLOB1 increased susceptibility, RNAi-silencing of CsLOB1 enhanced resistance to canker disease without impairing pathogen entry.”
e1“citrus bacterial canker, which is caused by Xanthomonas citri subsp. citri (Xcc)”
t0“susceptibility to citrus canker”
Claim · model-written summary
CsLOB1 RNAi silencing enhanced canker resistance without impairing pathogen entry.
- Treatment
- RNAi-silencing of CsLOB1e0
- Interactor
- Xanthomonas citri subsp. citri (Xcc)Xanthomonas citri pv. citrie1pathogen
- Outcome
- resistance to canker diseasee0
- Direction
- enhancede0
- Qualifier
- without impairing pathogen entrye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Whilst overexpression of CsLOB1 increased susceptibility, RNAi-silencing of CsLOB1 enhanced resistance to canker disease without impairing pathogen entry.”
e1“citrus bacterial canker, which is caused by Xanthomonas citri subsp. citri (Xcc)”
t0“susceptibility to citrus canker”
Claim · model-written summary
CsLOB1 positively regulated cell wall modification, cytokinin metabolism, and cell division.
- Gene
- CsLOB1e0
- Outcome
- cell wall degradation and modification processes, cytokinin metabolism, and cell divisione0
- Direction
- positively regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis revealed that CsLOB1 positively regulated cell wall degradation and modification processes, cytokinin metabolism, and cell division.”
t0“susceptibility to citrus canker”
Claim · model-written summary
ChIP-seq identified 565 CsLOB1-targeted genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Additionally, 565 CsLOB1-targeted genes were identified in chromatin immunoprecipitation-sequencing (ChIP-seq) experiments.”
t0“susceptibility to citrus canker”
Claim · model-written summary
The most overrepresented CsLOB1 binding sites carried the conserved GCGGCG motif.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Motif discovery analysis revealed that the most highly overrepresented binding sites had a conserved 6-bp 'GCGGCG' consensus DNA motif.”
e1“565 CsLOB1-targeted genes”
t0“susceptibility to citrus canker”
Claim · model-written summary
CsLOB1 was suggested to directly activate four cell wall remodeling genes.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq and ChIP-seq data suggested that CsLOB1 directly activates the expression of four genes involved in cell wall remodeling, and three genes that participate in cytokinin and brassinosteroid hormone pathways.”
t0“susceptibility to citrus canker”
Claim · model-written summary
CsLOB1 was suggested to directly activate three hormone pathway genes.
- Gene
- CsLOB1e0
- Outcome
- three genes that participate in cytokinin and brassinosteroid hormone pathwayse0
- Direction
- directly activatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNA-seq and ChIP-seq data suggested that CsLOB1 directly activates the expression of four genes involved in cell wall remodeling, and three genes that participate in cytokinin and brassinosteroid hormone pathways.”
t0“susceptibility to citrus canker”
The same questions in other papers
loss of function · molecular: metabolites · CitrusPositive and negative results
5 papers in the same direction as this paper · 5 in the opposite direction
- negative
- negative
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See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.