Abstract
Pre-harvest water deficit (PHWD) plays an important role in sugar accumulation of citrus fruit. However, the mechanism is not known well. Here, it was confirmed that PHWD promoted sucrose accumulation of citrus fruit, but had limited effect on fructose, glucose and total acid. A sucrose transporter, CsSUT1, which localizes to the plasma membrane, was demonstrated to function in sucrose transport induced by PHWD. Compared to wild-type, CsSUT1 overexpression in citrus calli stimulated sucrose, fructose and glucose accumulation, while its silencing in juice sacs reduced sucrose accumulation. Increased sugar accumulation in transgenic lines enhanced plant drought tolerance, and resulted in decreased electrolyte leakage, malondialdehyde and hydrogen peroxide contents, as well as increased superoxide dismutase activity and proline contents. An abscisic acid (ABA)-responsive transcription factor, CsABF3, was found with a same expression pattern with CsSUT1 under PHWD. Yeast one-hybrid, electrophoretic mobility shift assay and dual-luciferase assays all revealed that CsABF3 directly bound with the CsSUT1 promoter by ABA responsive elements. When CsABF3 was overexpressed in citrus calli, the sucrose, fructose and glucose concentration increased correspondingly. Further, transgenic studies demonstrated that CsABF3 could affect sucrose accumulation by regulating CsSUT1. Overall, this study revealed a regulation of CsABF3 promoting CsSUT1 expression and sucrose accumulation in response to PHWD. Our results provide a detail insight into the quality formation of citrus fruit.
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Claims 13
Claim · model-written summary
PHWD promoted sucrose accumulation in citrus fruit.
CitrusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“PHWD promoted sucrose accumulation of citrus fruit”
Claim · model-written summary
PHWD had limited effects on fructose, glucose and total acid.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“PHWD promoted sucrose accumulation of citrus fruit, but had limited effect on fructose, glucose and total acid”
Claim · model-written summary
CsSUT1 localized to the plasma membrane.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“CsSUT1, which localizes to the plasma membrane”
Claim · model-written summary
CsSUT1 functioned in PHWD-induced sucrose transport.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“CsSUT1, which localizes to the plasma membrane, was demonstrated to function in sucrose transport induced by PHWD”
Claim · model-written summary
CsSUT1 overexpression increased sucrose, fructose and glucose accumulation in citrus calli relative to wild type.
- Organ / stage
- callie0
- Treatment
- CsSUT1 overexpressione0
- Gene
- CsSUT1e0
- Outcome
- sucrose, fructose and glucose accumulatione0
- Direction
- stimulatede0
- Comparator
- wild-typee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to wild-type, CsSUT1 overexpression in citrus calli stimulated sucrose, fructose and glucose accumulation”
Claim · model-written summary
CsSUT1 silencing reduced sucrose accumulation in juice sacs.
- Organ / stage
- juice sacse0
- Treatment
- silencinge0
- Gene
- CsSUT1t0
- Outcome
- sucrose accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“CsABF3-activated CsSUT1 pathway is implicated in pre-harvest water deficit inducing sucrose accumulation in citrus fruit”
e0“its silencing in juice sacs reduced sucrose accumulation”
Claim · model-written summary
Increased sugar accumulation in transgenic lines enhanced drought tolerance.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“Increased sugar accumulation in transgenic lines enhanced plant drought tolerance”
Claim · model-written summary
Transgenic lines with increased sugar had decreased electrolyte leakage, malondialdehyde and hydrogen peroxide.
- Material
- transgenic linese0
- Outcome
- electrolyte leakage, malondialdehyde and hydrogen peroxide contentse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“Increased sugar accumulation in transgenic lines enhanced plant drought tolerance, and resulted in decreased electrolyte leakage, malondialdehyde and hydrogen peroxide contents”
Claim · model-written summary
Transgenic lines with increased sugar had higher superoxide dismutase activity and proline content.
- Material
- transgenic linese0
- Outcome
- superoxide dismutase activity and proline contentse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“Increased sugar accumulation in transgenic lines enhanced plant drought tolerance, and resulted in decreased electrolyte leakage, malondialdehyde and hydrogen peroxide contents, as well as increased superoxide dismutase activity and proline contents”
Claim · model-written summary
CsABF3 and CsSUT1 had the same expression pattern under PHWD.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“CsABF3, was found with a same expression pattern with CsSUT1 under PHWD”
Claim · model-written summary
CsABF3 directly bound the CsSUT1 promoter through ABA responsive elements.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“CsABF3 directly bound with the CsSUT1 promoter by ABA responsive elements”
Claim · model-written summary
CsABF3 overexpression increased sucrose, fructose and glucose concentrations in citrus calli.
- Treatment
- CsABF3 was overexpressede0
- Gene
- CsABF3e0
- Outcome
- sucrose, fructose and glucose concentratione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When CsABF3 was overexpressed in citrus calli, the sucrose, fructose and glucose concentration increased correspondingly”
Claim · model-written summary
CsABF3 regulated CsSUT1 and thereby affected sucrose accumulation.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“citrus fruit”
e0“CsABF3 could affect sucrose accumulation by regulating CsSUT1”
The same questions in other papers
drought · molecular: metabolites · CitrusResults differ
2 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
1 paper more in this group
See all 5 papers →loss of function · molecular: metabolites · CitrusPositive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.