Abstract
RGB1, a subunit of heterotrimeric G protein, plays important roles in regulating grain size and weight of rice. However, the molecular mechanisms underlying controlling grain filling process by G protein are still largely unclear. In the present study, we show that RGB1 controls not only the grain size but also the grain filling process. Knock-down of RGB1 significantly delayed grain development and reduced starch accumulation and grain weight, which was closely related to the delayed and the lower expression of genes encoding sucrose metabolism and starch biosynthesis related enzymes during grain filling stage. Suppression of RGB1 expression also resulted in the lower auxin content in grains, which was correlated with the lower expression of OsNF-YB1 and OsYUC11 during grain filling stage. Further biochemical evidence showed that OsYUC11 expression was under control of OsNF-YB1 by its interaction with promoter of OsYUC11 . Taken together, we propose that RGB1 controls rice grain development and grain filling process by changing auxin homeostasis in endosperm cells. OsNF-YB1, which acts as a key downstream effector of RGB1, interacts directly with the promoter of OsYUC11 and stimulates the OsYUC11 expression, thereby regulating auxin biosynthesis and starch accumulation and grain size.
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Claims 12
Claim · model-written summary
RGB1 knock-down significantly delayed rice grain development.
- Taxon
- Rice
Oryza sativat0 - Treatment
- Knock-down of RGB1e0
- Gene
- RGB1e0
- Outcome
- grain developmente0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Knock-down of RGB1 significantly delayed grain development”
Claim · model-written summary
RGB1 knock-down significantly reduced starch accumulation.
- Taxon
- RiceOryza sativat0
- Treatment
- Knock-down of RGB1e0
- Gene
- RGB1e0
- Outcome
- starch accumulatione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Knock-down of RGB1 significantly delayed grain development and reduced starch accumulation and grain weight”
Claim · model-written summary
RGB1 knock-down significantly reduced grain weight.
- Taxon
- RiceOryza sativat0
- Treatment
- Knock-down of RGB1e0
- Gene
- RGB1e0
- Outcome
- grain weighte0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Knock-down of RGB1 significantly delayed grain development and reduced starch accumulation and grain weight”
Claim · model-written summary
RGB1 knock-down was linked to delayed expression of sucrose metabolism and starch biosynthesis enzyme genes.
- Taxon
- RiceOryza sativat0
- Organ / stage
- grain filling stagee0
- Outcome
- expression of genes encoding sucrose metabolism and starch biosynthesis related enzymese0
- Direction
- delayede0
Not reportedGeneTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“the delayed and the lower expression of genes encoding sucrose metabolism and starch biosynthesis related enzymes during grain filling stage”
Claim · model-written summary
Expression of sucrose metabolism and starch biosynthesis enzyme genes was lower during grain filling.
- Taxon
- RiceOryza sativat0
- Organ / stage
- grain filling stagee0
- Outcome
- expression of genes encoding sucrose metabolism and starch biosynthesis related enzymese0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“the delayed and the lower expression of genes encoding sucrose metabolism and starch biosynthesis related enzymes during grain filling stage”
Claim · model-written summary
Suppression of RGB1 expression lowered grain auxin content.
- Taxon
- RiceOryza sativat0
- Treatment
- Suppression of RGB1 expressione0
- Gene
- RGB1e0
- Outcome
- auxin content in grainse0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“Suppression of RGB1 expression also resulted in the lower auxin content in grains”
Claim · model-written summary
Suppression of RGB1 expression was correlated with lower OsNF-YB1 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- Suppression of RGB1 expressione1
- Gene
- OsNF-YB1e0
- Outcome
- expression of OsNF-YB1e0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“which was correlated with the lower expression of OsNF-YB1 and OsYUC11 during grain filling stage”
e1“Suppression of RGB1 expression”
Claim · model-written summary
Suppression of RGB1 expression was correlated with lower OsYUC11 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- Suppression of RGB1 expressione1
- Gene
- OsYUC11e0
- Outcome
- OsYUC11e0
- Direction
- lowere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“which was correlated with the lower expression of OsNF-YB1 and OsYUC11 during grain filling stage”
e1“Suppression of RGB1 expression”
Claim · model-written summary
OsNF-YB1 interacts with the OsYUC11 promoter and controls OsYUC11 expression.
- Taxon
- RiceOryza sativat0
- Gene
- OsNF-YB1e0
- Outcome
- OsYUC11 expressione0
- Qualifier
- interaction with promoter of OsYUC11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“OsYUC11 expression was under control of OsNF-YB1 by its interaction with promoter of OsYUC11”
Claim · model-written summary
The authors propose that RGB1 controls grain development and filling through auxin homeostasis in endosperm cells.
- Taxon
- riceOryza sativae0
- Gene
- RGB1e0
- Outcome
- grain development and grain filling processe0
- Qualifier
- changing auxin homeostasis in endosperm cellse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RGB1 controls rice grain development and grain filling process by changing auxin homeostasis in endosperm cells”
Claim · model-written summary
OsNF-YB1 stimulates OsYUC11 expression.
- Taxon
- RiceOryza sativat0
- Gene
- OsNF-YB1e0
- Outcome
- OsYUC11 expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“OsNF-YB1, which acts as a key downstream effector of RGB1, interacts directly with the promoter of OsYUC11 and stimulates the OsYUC11 expression”
Claim · model-written summary
The proposed OsNF-YB1–OsYUC11 pathway regulates auxin biosynthesis, starch accumulation, and grain size.
- Taxon
- RiceOryza sativat0
- Gene
- OsNF-YB1e1
- Outcome
- auxin biosynthesis and starch accumulation and grain sizee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Rice”
e0“thereby regulating auxin biosynthesis and starch accumulation and grain size”
e1“OsNF-YB1, which acts as a key downstream effector of RGB1, interacts directly with the promoter of OsYUC11”
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