Paper · 2021 · Molecules

Analysis of Chemical Composition and In Vitro and In Vivo Antifungal Activity of Raphanus raphanistrum Extracts against Fusarium and Pythiaceae, Affecting Apple and Peach Seedlings

Sabrine Mannai; Najwa Benfradj; Ahlem Karoui; et al.

5
claims
11
verbatim quotes
8
citations
3
research areas
✓
Open access · cc-by

Abstract

The goal of this investigation was to evaluate the in vitro and in vivo efficiency of Raphanus raphanistrum extracts against Fusarium and Pythiaceae species associated with apple and peach seedling decline in Tunisian nurseries. A chemical composition of organic extracts was accomplished using liquid chromatography, thin layer chromatography, and gas chromatography analysis. The in vitro test of three aqueous extract doses of R. raphanistrum against some apple and peach decline agents showed its efficacy in reducing mycelia growth. The in vivo assay of fine powder of this plant on peach seedlings revealed that treatment 8-weeks before the inoculation and planting was more efficient than the treatment before one week. This experiment revealed that the root weight of peach seedlings inoculated by F. oxysporum was improved to 207.29%. For apple seedlings, the treatment 8 weeks before the inoculation and plantation was more efficient than the treatment one week before; it reduced the root browning index. The study of R. raphanistrum chemical composition and its efficiency showed that the glucosinolates products: nitrile (4-Hydroxy-3-(4-methylphenylthio) butane nitrile, benzene acetonitrile, 4-fluoro,butane nitrile, 4-hydroxy-3-[(4-methylphenyl) thio] nitrile), and thiocyanate molecules (thiocyanic acid, ethyle) are responsible for the anti-fungal activities.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Raphanus aqueous extract reduced growth of apple and peach decline agents in vitro.

Treatment
three aqueous extract doses of R. raphanistrume0
Interactor
apple and peach decline agentse0pathogen
Outcome
mycelia growthe0
Direction
reducinge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“three aqueous extract doses of R. raphanistrum against some apple and peach decline agents showed its efficacy in reducing mycelia growth”

✓ located verbatim in the source · abstract, characters 399–536 · show in text ↑
t0

“Apple and Peach Seedlings”

✓ located verbatim in the source · title, characters 155–180 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Raphanus powder applied eight weeks before inoculation was more effective on peach seedlings than one week before.

Taxon
peach seedlingsPrunus persicae0
Treatment
treatment 8-weeks before the inoculation and plantinge0
Outcome
more efficiente0
Direction
more efficiente0
Comparator
treatment before one weeke0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“fine powder of this plant on peach seedlings revealed that treatment 8-weeks before the inoculation and planting was more efficient than the treatment before one week”

✓ located verbatim in the source · abstract, characters 559–725 · show in text ↑
t0

“Apple and Peach Seedlings”

✓ located verbatim in the source · title, characters 155–180 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Peach seedling root weight improved to 207.29% following inoculation with F. oxysporum under the powder treatment.

Taxon
peach seedlingsPrunus persicae0
Treatment
R. raphanistrume1
Interactor
F. oxysporumFusarium oxysporume0pathogen
Outcome
root weighte0
Direction
improvede0
Value
207.29%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗
Fusarium oxysporum
Fusarium oxysporum · InteractorClemson University - USDA Cooperative Extension Slide Series · CC BY 3.0 · Wikimedia Commons ↗

Verbatim

e0

“root weight of peach seedlings inoculated by F. oxysporum was improved to 207.29%”

✓ located verbatim in the source · abstract, characters 761–842 · show in text ↑
t0

“Apple and Peach Seedlings”

✓ located verbatim in the source · title, characters 155–180 · show in text ↑
e1

“The study of R. raphanistrum chemical composition”

✓ located verbatim in the source · abstract, characters 1016–1065 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

For apple seedlings, treatment eight weeks before inoculation reduced root browning more than treatment one week before.

Taxon
apple seedlingsMalus domesticae0
Treatment
treatment 8 weeks before the inoculation and plantatione0
Outcome
root browning indexe0
Direction
reducede0
Comparator
treatment one week beforee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗

Verbatim

e0

“For apple seedlings, the treatment 8 weeks before the inoculation and plantation was more efficient than the treatment one week before; it reduced the root browning index”

✓ located verbatim in the source · abstract, characters 844–1014 · show in text ↑
t0

“Apple and Peach Seedlings”

✓ located verbatim in the source · title, characters 155–180 · show in text ↑
Mechanismauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The authors attribute antifungal activity to glucosinolate derived nitrile and thiocyanate molecules.

Taxon
Apple and Peach SeedlingsMalus domesticaPrunus persicat0
Compound
glucosinolates productse0
Outcome
anti-fungal activitiese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Malus domestica
Malus domestica · TaxonW.carter · CC0 · Wikimedia Commons ↗
Prunus persica
Prunus persica · TaxonAllagash Brewing · CC BY 2.0 · Wikimedia Commons ↗

Verbatim

e0

“glucosinolates products: nitrile (4-Hydroxy-3-(4-methylphenylthio) butane nitrile, benzene acetonitrile, 4-fluoro,butane nitrile, 4-hydroxy-3-[(4-methylphenyl) thio] nitrile), and thiocyanate molecules (thiocyanic acid, ethyle) are responsible for the anti-fungal activities”

✓ located verbatim in the source · abstract, characters 1101–1375 · show in text ↑
t0

“Apple and Peach Seedlings”

✓ located verbatim in the source · title, characters 155–180 · show in text ↑

botanical · growth · Prunus persica (peach)Positive and negative results

8 papers: 5 positive, 3 negative or no effect

4 papers in the same direction as this paper · 3 in the opposite direction

  1. negative
  2. negative
  3. negative

4 papers more in this group

See all 8 papers →

botanical · growth · Malus domestica (apple)Positive and negative results

17 papers: 15 positive, 2 negative or no effect

14 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

13 papers more in this group

See all 17 papers →

inoculation · disease · Malus domestica (apple)Positive and negative results

63 papers: 23 positive, 40 negative or no effect

22 papers in the same direction as this paper · 40 in the opposite direction

  1. negative
  2. negative
  3. negative

59 papers more in this group

See all 63 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.