Abstract
Nitrogen concentration affects growth and bolting of plants, but its regulation mechanism is still unclear. In this work, three nitrate concentration treatments (5%, 100%, 200%) in nutrient solution were conducted to explore the internal relationship between nitrogen and bolting in flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering. Low-nitrate treatment reduced the content of nitrate, soluble protein, free amino acid and total nitrogen, and increased the C/N ratio. The C/N ratio was significantly negatively correlated with plant height, stem thickness and biomass, while it was significantly positively correlated with flowering rate. Thus, it was indicated that nitrogen may affect bolting and flowering by regulating the C/N ratio of flowering Chinese cabbage plants. The expression of flowering-related genes (SOC1, LFY) was increased significantly under low nitrate treatment. In addition, the pith cell area at the stem tip was significantly reduced under low nitrate treatment, resulting in a significant decrease in stem thickness. The expression of cyclin- and expansin-related genes (CYCD3-3, CYCB1-1 and EXPA8) was significantly reduced, which indicated that nitrogen may regulate the stem development of flowering Chinese cabbage by regulating the expression of cyclin- and expansin-related genes.
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Claims 12
Claim · model-written summary
low nitrate treatment advanced bolting and flowering time in flowering Chinese cabbage
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Treatment
- low nitratee1
- Outcome
- bolting and flowering timee1
- Direction
- earliere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
Claim · model-written summary
low-nitrate treatment reduced the content of nitrate, soluble protein and free amino acid
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Treatment
- Low-nitrate treatmente2
- Outcome
- content of nitrate, soluble protein, free amino acide2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e2“Low-nitrate treatment reduced the content of nitrate, soluble protein, free amino acid and total nitrogen, and increased the C/N ratio”
Claim · model-written summary
low-nitrate treatment reduced the total nitrogen content of flowering Chinese cabbage
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Treatment
- Low-nitrate treatmente2
- Outcome
- total nitrogene2
- Direction
- reducede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e2“Low-nitrate treatment reduced the content of nitrate, soluble protein, free amino acid and total nitrogen, and increased the C/N ratio”
Claim · model-written summary
low-nitrate treatment increased the C/N ratio of flowering Chinese cabbage
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Treatment
- Low-nitrate treatmente2
- Outcome
- C/N ratioe2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e2“Low-nitrate treatment reduced the content of nitrate, soluble protein, free amino acid and total nitrogen, and increased the C/N ratio”
Claim · model-written summary
the C/N ratio was significantly negatively correlated with plant height, stem thickness and biomass
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Compound
- C/N ratioe3
- Outcome
- plant height, stem thickness and biomasse3
- Direction
- negatively correlatede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e3“The C/N ratio was significantly negatively correlated with plant height, stem thickness and biomass, while it was significantly positively correlated with flowering rate”
Claim · model-written summary
the C/N ratio was significantly positively correlated with flowering rate
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Compound
- C/N ratioe3
- Outcome
- flowering ratee3
- Direction
- positively correlatede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e3“The C/N ratio was significantly negatively correlated with plant height, stem thickness and biomass, while it was significantly positively correlated with flowering rate”
Claim · model-written summary
nitrogen may affect bolting and flowering by regulating the C/N ratio
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise4 - Compound
- C/N ratioe4
- Outcome
- bolting and floweringe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e4“Thus, it was indicated that nitrogen may affect bolting and flowering by regulating the C/N ratio of flowering Chinese cabbage plants”
Claim · model-written summary
low nitrate treatment significantly increased the expression of flowering-related genes SOC1 and LFY
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Treatment
- low nitrate treatmente5
- Gene
- SOC1, LFYe5
- Outcome
- expression of flowering-related genese5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e5“The expression of flowering-related genes (SOC1, LFY) was increased significantly under low nitrate treatment”
Claim · model-written summary
low nitrate treatment significantly reduced the pith cell area at the stem tip
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Organ / stage
- pithe6
- Treatment
- low nitrate treatmente6
- Outcome
- pith cell area at the stem tipe6
- Direction
- reducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e6“In addition, the pith cell area at the stem tip was significantly reduced under low nitrate treatment, resulting in a significant decrease in stem thickness”
Claim · model-written summary
low nitrate treatment significantly decreased stem thickness of flowering Chinese cabbage
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Treatment
- low nitrate treatmente6
- Outcome
- stem thicknesse6
- Direction
- decreasee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e6“In addition, the pith cell area at the stem tip was significantly reduced under low nitrate treatment, resulting in a significant decrease in stem thickness”
Claim · model-written summary
low nitrate treatment significantly reduced the expression of cyclin- and expansin-related genes CYCD3-3, CYCB1-1 and EXPA8
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise1 - Treatment
- low nitrate treatmente6
- Gene
- CYCD3-3, CYCB1-1 and EXPA8e7
- Outcome
- expression of cyclin- and expansin-related genese7
- Direction
- reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“flowering Chinese cabbage. The results showed that the bolting and flowering time was earlier under the treatment with low nitrate and, the lower the nitrate concentration, the earlier the bolting and flowering”
e6“In addition, the pith cell area at the stem tip was significantly reduced under low nitrate treatment, resulting in a significant decrease in stem thickness”
e7“The expression of cyclin- and expansin-related genes (CYCD3-3, CYCB1-1 and EXPA8) was significantly reduced”
Claim · model-written summary
nitrogen may regulate stem development of flowering Chinese cabbage by regulating cyclin- and expansin-related gene expression
- Taxon
- flowering Chinese cabbage
Brassica rapa subsp. pekinensise7 - Gene
- cyclin- and expansin-related genese7
- Outcome
- stem developmente7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e7“nitrogen may regulate the stem development of flowering Chinese cabbage by regulating the expression of cyclin- and expansin-related genes”
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