Abstract
Selenium (Se) is suggested as an emerging pollutant in agricultural environment because of the increasing anthropogenic release of Se, which in turn results in phytotoxicity. The most common consequence of Se-induced toxicity in plants is oxidative injury, but how Se induces reactive oxygen species (ROS) burst remains unclear. In this work, histofluorescent staining was applied to monitor the dynamics of ROS and nitric oxide (NO) in the root of Brassica rapa under Se(IV) stress. Se(IV)-induced faster accumulation of NO than ROS. Both NO and ROS accumulation were positively correlated with Se(IV)-induced inhibition of root growth. The NO accumulation was nitrate reductase (NR)- and nitric oxide synthase (NOS)-dependent while ROS accumulation was NADPH oxidase-dependent. The removal of NO by NR inhibitor, NOS inhibitor, and NO scavenger could alleviate Se(IV)-induced expression of Br_Rbohs coding for NADPH oxidase and the following ROS accumulation in roots, which further resulted in the amelioration of Se(IV)-induced oxidative injury and growth inhibition. Thus, we proposed that the endogenous NO played a toxic role in B. rapa under Se(IV) stress by triggering ROS burst. Such findings can be used to evaluate the toxic effects of Se contamination on crop plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Se(IV) induced faster accumulation of NO than ROS in B. rapa roots.
- Taxon
- Brassica rapa
Brassica rapae0 - Treatment
- Se(IV)e1
- Outcome
- accumulation of NOe1
- Direction
- fastere1
- Comparator
- ROSe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e1“Se(IV)-induced faster accumulation of NO than ROS”
Claim · model-written summary
NO accumulation was positively correlated with Se(IV)-induced root growth inhibition in B. rapa.
- Taxon
- Brassica rapaBrassica rapae0
- Treatment
- Se(IV)e2
- Compound
- NOe2
- Outcome
- inhibition of root growthe2
- Direction
- positivelye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e2“Both NO and ROS accumulation were positively correlated with Se(IV)-induced inhibition of root growth”
Claim · model-written summary
ROS accumulation was positively correlated with Se(IV)-induced root growth inhibition in B. rapa.
- Taxon
- Brassica rapaBrassica rapae0
- Treatment
- Se(IV)e2
- Compound
- ROSe2
- Outcome
- inhibition of root growthe2
- Direction
- positivelye2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e2“Both NO and ROS accumulation were positively correlated with Se(IV)-induced inhibition of root growth”
Claim · model-written summary
NO accumulation was dependent on nitrate reductase (NR) in B. rapa roots.
- Taxon
- Brassica rapaBrassica rapae0
- Gene
- nitrate reductase (NR)e3
- Outcome
- NO accumulatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e3“The NO accumulation was nitrate reductase (NR)- and nitric oxide synthase (NOS)-dependent”
Claim · model-written summary
NO accumulation was dependent on nitric oxide synthase (NOS) in B. rapa roots.
- Taxon
- Brassica rapaBrassica rapae0
- Gene
- nitric oxide synthase (NOS)e3
- Outcome
- NO accumulatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e3“The NO accumulation was nitrate reductase (NR)- and nitric oxide synthase (NOS)-dependent”
Claim · model-written summary
ROS accumulation was dependent on NADPH oxidase in B. rapa roots.
- Taxon
- Brassica rapaBrassica rapae0
- Gene
- NADPH oxidasee4
- Outcome
- ROS accumulatione4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e4“while ROS accumulation was NADPH oxidase-dependent”
Claim · model-written summary
Removing NO with NR inhibitor, NOS inhibitor and NO scavenger could alleviate Se(IV)-induced expression of Br_Rbohs.
- Taxon
- Brassica rapaBrassica rapae0
- Treatment
- NR inhibitor, NOS inhibitor, and NO scavengere5
- Gene
- Br_Rbohse5
- Outcome
- expression of Br_Rbohse5
- Direction
- alleviatee5
- Comparator
- Se(IV)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e5“The removal of NO by NR inhibitor, NOS inhibitor, and NO scavenger could alleviate Se(IV)-induced expression of Br_Rbohs coding for NADPH oxidase and the following ROS accumulation in roots, which further resulted in the amelioration of Se(IV)-induced oxidative injury and growth inhibition”
Claim · model-written summary
Removing NO with NR inhibitor, NOS inhibitor and NO scavenger could alleviate Se(IV)-induced ROS accumulation in roots.
- Taxon
- Brassica rapaBrassica rapae0
- Treatment
- NR inhibitor, NOS inhibitor, and NO scavengere5
- Outcome
- ROS accumulation in rootse5
- Direction
- alleviatee5
- Comparator
- Se(IV)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e5“The removal of NO by NR inhibitor, NOS inhibitor, and NO scavenger could alleviate Se(IV)-induced expression of Br_Rbohs coding for NADPH oxidase and the following ROS accumulation in roots, which further resulted in the amelioration of Se(IV)-induced oxidative injury and growth inhibition”
Claim · model-written summary
Removing NO with NR inhibitor, NOS inhibitor and NO scavenger ameliorated Se(IV)-induced oxidative injury and growth inhibition.
- Taxon
- Brassica rapaBrassica rapae0
- Treatment
- NR inhibitor, NOS inhibitor, and NO scavengere5
- Outcome
- oxidative injury and growth inhibitione5
- Direction
- amelioratione5
- Comparator
- Se(IV)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the root of Brassica rapa under Se(IV) stress”
e5“The removal of NO by NR inhibitor, NOS inhibitor, and NO scavenger could alleviate Se(IV)-induced expression of Br_Rbohs coding for NADPH oxidase and the following ROS accumulation in roots, which further resulted in the amelioration of Se(IV)-induced oxidative injury and growth inhibition”
Claim · model-written summary
Endogenous NO is proposed to play a toxic role in B. rapa under Se(IV) stress by triggering ROS burst.
- Taxon
- B. rapaBrassica rapae6
- Compound
- endogenous NOe6
- Outcome
- ROS burste6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Thus, we proposed that the endogenous NO played a toxic role in B. rapa under Se(IV) stress by triggering ROS burst”
The same questions in other papers
nitric oxide · molecular: expression · Brassica rapa (field mustard)Results agree
1 paper in the same direction as this paper
- positive
nitric oxide · physiology: oxidative · Brassica rapa (field mustard)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →nitrogen · molecular: expression · Brassica rapa (field mustard)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
nitrogen · physiology: oxidative · Brassica rapa (field mustard)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.