Abstract
During crop cultivation, water-deficit conditions retard growth, thus reducing crop productivity. Therefore, uncovering the mechanisms behind drought tolerance is a critical task for crop improvement. Here, we show that the rice (Oryza sativa) WRKY transcription factor OsWRKY5 negatively regulates drought tolerance. We determined that OsWRKY5 was mainly expressed in developing leaves at the seedling and heading stages, and that its expression was reduced by drought stress and by treatment with NaCl, mannitol, and abscisic acid (ABA). Notably, the genome-edited loss-of-function alleles oswrky5-2 and oswrky5-3 conferred enhanced drought tolerance, measured as plant growth under water-deficit conditions. Conversely, the overexpression of OsWRKY5 in the activation-tagged line oswrky5-D resulted in higher susceptibility under the same conditions. The loss of OsWRKY5 activity increased sensitivity to ABA, thus promoting ABA-dependent stomatal closure. Transcriptome deep sequencing and reverse transcription quantitative polymerase chain reaction analyses demonstrated that the expression of abiotic stress-related genes including rice MYB2 (OsMYB2) was upregulated in oswrky5 knockout mutants and downregulated in oswrky5-D mutants. Moreover, dual-luciferase, yeast one-hybrid, and chromatin immunoprecipitation assays showed that OsWRKY5 directly binds to the W-box sequences in the promoter region of OsMYB2 and represses OsMYB2 expression, thus downregulating genes downstream of OsMYB2 in the ABA signaling pathways. Our results demonstrate that OsWRKY5 functions as a negative regulator of ABA-induced drought stress tolerance, strongly suggesting that inactivation of OsWRKY5 or manipulation of key OsWRKY5 targets could be useful to improve drought tolerance in rice cultivars.
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Claims 10
Claim · model-written summary
The rice WRKY transcription factor OsWRKY5 negatively regulates drought tolerance.
- Taxon
- rice
Oryza sativae0 - Gene
- OsWRKY5e0
- Outcome
- drought tolerancee0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the rice (Oryza sativa) WRKY transcription factor OsWRKY5 negatively regulates drought tolerance”
Claim · model-written summary
OsWRKY5 was mainly expressed in developing leaves at the seedling and heading stages.
- Taxon
- riceOryza sativae0
- Organ / stage
- developing leaves at the seedling and heading stagese1
- Gene
- OsWRKY5e1
- Outcome
- expressede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa)”
e1“OsWRKY5 was mainly expressed in developing leaves at the seedling and heading stages”
Claim · model-written summary
Genome-edited loss-of-function alleles oswrky5-2 and oswrky5-3 conferred enhanced drought tolerance, measured as plant growth under water-deficit conditions.
- Taxon
- riceOryza sativae0
- Material
- oswrky5-2 and oswrky5-3e1
- Treatment
- water-deficit conditionse1
- Outcome
- drought tolerancee1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa)”
e1“Notably, the genome-edited loss-of-function alleles oswrky5-2 and oswrky5-3 conferred enhanced drought tolerance, measured as plant growth under water-deficit conditions”
Claim · model-written summary
Overexpression of OsWRKY5 in the activation-tagged line oswrky5-D resulted in higher susceptibility under water-deficit conditions.
- Taxon
- riceOryza sativae0
- Material
- oswrky5-De1
- Treatment
- overexpression of OsWRKY5e1
- Outcome
- susceptibilitye1
- Direction
- highere1
- Setting
- the same conditionse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa)”
e1“the overexpression of OsWRKY5 in the activation-tagged line oswrky5-D resulted in higher susceptibility under the same conditions”
Claim · model-written summary
Loss of OsWRKY5 activity increased sensitivity to ABA in rice.
- Taxon
- riceOryza sativae0
- Treatment
- The loss of OsWRKY5 activitye1
- Outcome
- sensitivity to ABAe1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa)”
e1“The loss of OsWRKY5 activity increased sensitivity to ABA, thus promoting ABA-dependent stomatal closure”
Claim · model-written summary
Loss of OsWRKY5 activity promoted ABA-dependent stomatal closure in rice.
- Taxon
- riceOryza sativae0
- Treatment
- The loss of OsWRKY5 activitye1
- Outcome
- ABA-dependent stomatal closuree1
- Direction
- promotinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa)”
e1“The loss of OsWRKY5 activity increased sensitivity to ABA, thus promoting ABA-dependent stomatal closure”
Claim · model-written summary
Expression of abiotic stress-related genes including OsMYB2 was upregulated in oswrky5 knockout mutants.
- Taxon
- riceOryza sativae0
- Treatment
- oswrky5 knockout mutantse1
- Gene
- rice MYB2e1
- Outcome
- expression of abiotic stress-related genese1
- Direction
- upregulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa)”
e1“the expression of abiotic stress-related genes including rice MYB2 (OsMYB2) was upregulated in oswrky5 knockout mutants and downregulated in oswrky5-D mutants”
Claim · model-written summary
Expression of abiotic stress-related genes including OsMYB2 was downregulated in oswrky5-D mutants.
- Taxon
- riceOryza sativae0
- Treatment
- oswrky5-D mutantse1
- Gene
- rice MYB2e1
- Outcome
- expression of abiotic stress-related genese1
- Direction
- downregulatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa)”
e1“the expression of abiotic stress-related genes including rice MYB2 (OsMYB2) was upregulated in oswrky5 knockout mutants and downregulated in oswrky5-D mutants”
Claim · model-written summary
OsWRKY5 directly binds the W-box sequences in the OsMYB2 promoter and represses OsMYB2 expression, thereby downregulating genes downstream of OsMYB2 in ABA signaling.
- Taxon
- riceOryza sativae0
- Gene
- OsWRKY5e1
- Outcome
- OsMYB2 expressione1
- Direction
- repressese1
- Qualifier
- directly binds to the W-box sequences in the promoter region of OsMYB2e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice (Oryza sativa)”
e1“OsWRKY5 directly binds to the W-box sequences in the promoter region of OsMYB2 and represses OsMYB2 expression, thus downregulating genes downstream of OsMYB2 in the ABA signaling pathways”
Claim · model-written summary
Inactivation of OsWRKY5 or manipulation of its key targets could be useful to improve drought tolerance in rice cultivars.
- Taxon
- riceOryza sativae0
- Outcome
- improve drought tolerancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inactivation of OsWRKY5 or manipulation of key OsWRKY5 targets could be useful to improve drought tolerance in rice cultivars”
The same questions in other papers
drought · tolerance: abiotic · Oryza sativa (rice)Positive and negative results
13 papers in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- negative
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See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.