Abstract
Seed ageing is associated with a high concentration of reactive oxygen species (ROS). Apple (Malus domestica Borkh.) seeds belong to the orthodox type. Due to a deep dormancy, they may be stored in dry condition at 5 °C for a long time, without viability loss. In the laboratory, artificial ageing of apple seeds is performed by imbibition in wet sand at warm temperature (33 °C). The aim of the work was to study nitric oxide (NO) as a seed vigour preservation agent. Embryos isolated from apple seeds subjected to accelerated ageing for 7, 14, 21 or 40 days were fumigated with NO. Embryo quality was estimated by TTC and MDA tests. ROS level was confirmed by NBT staining. We analysed the alteration in transcript levels of CAT, SOD and POX. NO fumigation of embryos of seeds aged for 21 days stimulated germination and increased ROS level which correlated to the elevated expression of RBOH. The increased total antioxidant capacity after NO fumigation was accompanied by the increased transcript levels of genes encoding enzymatic antioxidants, that could protect against ROS overaccumulation. Moreover, post-aged NO application diminished the nitro-oxidative modification of RNA, proving NO action as a remedy in oxidative remodelling after seeds ageing.
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Claims 6
Claim · model-written summary
NO fumigation stimulated germination of embryos from seeds aged 21 days.
- Taxon
- Apple
Malus domesticat0 - Treatment
- NO fumigatione0
- Outcome
- germinatione0
- Direction
- stimulatede0
- Qualifier
- aged for 21 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO fumigation of embryos of seeds aged for 21 days stimulated germination”
t0“Apple”
Claim · model-written summary
NO fumigation increased ROS level in embryos from seeds aged 21 days.
- Taxon
- AppleMalus domesticat0
- Treatment
- NO fumigatione0
- Outcome
- ROS levele0
- Direction
- increasede0
- Qualifier
- aged for 21 dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO fumigation of embryos of seeds aged for 21 days stimulated germination and increased ROS level”
t0“Apple”
Claim · model-written summary
Increased ROS was correlated with elevated RBOH expression.
- Taxon
- AppleMalus domesticat0
- Gene
- RBOHe0
- Outcome
- ROS levele0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased ROS level which correlated to the elevated expression of RBOH”
t0“Apple”
Claim · model-written summary
NO fumigation increased total antioxidant capacity.
- Taxon
- AppleMalus domesticat0
- Treatment
- NO fumigatione0
- Outcome
- total antioxidant capacitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increased total antioxidant capacity after NO fumigation”
t0“Apple”
Claim · model-written summary
NO fumigation increased transcript levels of genes encoding enzymatic antioxidants.
- Taxon
- AppleMalus domesticat0
- Treatment
- NO fumigatione0
- Gene
- genes encoding enzymatic antioxidantse0
- Outcome
- transcript levelse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after NO fumigation was accompanied by the increased transcript levels of genes encoding enzymatic antioxidants”
t0“Apple”
Claim · model-written summary
Post-aged NO application diminished nitro-oxidative RNA modification.
- Taxon
- AppleMalus domesticat0
- Treatment
- post-aged NO applicatione0
- Outcome
- nitro-oxidative modification of RNAe0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“post-aged NO application diminished the nitro-oxidative modification of RNA”
t0“Apple”
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