Abstract
Abstract The research was undertaken to obtain drought-tolerant patchouli putative mutant calli through gamma-irradiation and in-vitro selection using Polyethylene Glycol (PEG). Patchoulina 2 variety was used as the mother plant. Embryogenic calli were induced on three medium formulation (combination of 0.1; 0.3 and 0.5 mg/l 2.4D with 0.1 mg/l BAP). The induced embryogenic calli were then exposed to nine levels of Gamma irradiation (0, 5, 10, 15, 20, 25, 30, 35, and 40 Gray) in combination with two levels of PEG (0 and 20%). The results showed that MS media with 2.4-D 0.3 mg/l and BAP 0.1 mg/l was the best medium for callus induction. The ability of calli to survive decreased with increasing doses of gamma irradiation. The radiosensitivity of patchouli calli showed that LD 20 at 14.06 Gray and LD 50 at 26.98 Gray. The drought-tolerant patchouli putative mutant calli were obtained from gamma-ray irradiation doses 15, 20, and 25 Gray in selection media 20% PEG has better drought stress tolerance compared to parent Patchoulina 2 variety. Further selection in the glasshouse and field are needed to obtain a candidate variety of patchouli drought tolerance.
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Claims 6
Claim · model-written summary
MS medium containing 0.3 mg/l 2.4-D and 0.1 mg/l BAP was best for callus induction.
- Taxon
- patchouli
Pogostemon cablint0 - Treatment
- MS media with 2.4-D 0.3 mg/l and BAP 0.1 mg/le0
- Outcome
- callus inductione0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MS media with 2.4-D 0.3 mg/l and BAP 0.1 mg/l was the best medium for callus induction”
t0“patchouli”
Claim · model-written summary
Callus survival declined as gamma irradiation dose increased.
- Taxon
- patchouliPogostemon cablint0
- Treatment
- gamma irradiatione0
- Outcome
- ability of calli to survivee0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ability of calli to survive decreased with increasing doses of gamma irradiation”
t0“patchouli”
Claim · model-written summary
Patchouli callus LD20 was 14.06 Gray.
- Taxon
- patchouliPogostemon cablint0
- Outcome
- LD 20e0
- Value
- 14.06 Graye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LD 20 at 14.06 Gray”
t0“patchouli”
Claim · model-written summary
Patchouli callus LD50 was 26.98 Gray.
- Taxon
- patchouliPogostemon cablint0
- Outcome
- LD 50e0
- Value
- 26.98 Graye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LD 50 at 26.98 Gray”
t0“patchouli”
Claim · model-written summary
Mutant calli obtained at 15, 20, and 25 Gray in 20% PEG had better drought tolerance than parent Patchoulina 2.
- Taxon
- patchouliPogostemon cablint0
- Treatment
- gamma-ray irradiation doses 15, 20, and 25 Gray in selection media 20% PEGe0
- Outcome
- drought stress tolerancee0
- Direction
- bettere0
- Comparator
- parent Patchoulina 2 varietye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gamma-ray irradiation doses 15, 20, and 25 Gray in selection media 20% PEG has better drought stress tolerance compared to parent Patchoulina 2 variety”
t0“patchouli”
Claim · model-written summary
The authors recommend further greenhouse and field selection.
- Taxon
- patchouliPogostemon cablint0
- Outcome
- Further selection in the glasshouse and fielde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further selection in the glasshouse and field are needed to obtain a candidate variety of patchouli drought tolerance”
t0“patchouli”
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