Abstract
A temporary immersion system (TIS) bioreactor has been used as an efficient and cost-effective method for the in vitro propagation of many plant species. In the current study, the applicability of a TIS bioreactor for plantlet regeneration Chrysanthemum morifolium Ramat., Fragaria × ananassa Duch., and Cnidium officinale Makino was studied. Shoot length, a number of leaves per regenerated shoot, fresh, and dry biomass of plantlets were optimal with the TIS compared to semi-solid and liquid immersion cultures. The leaf area in cryshanthmum, strawberry, and C. afficinale were 2.87 cm2, 3.51 cm2, and 1.43 cm2, respectively, in the plants regenerated by TIS. The photosynthetic pigments were highest in strawberry plants grown in TIS bioreactor culture, and there was no significant difference between semi-solid and liquid culture while the highest values were obtained in C. officinale maintained in semi-solid culture. The chrysanthemum and strawberry plants showed a 100% acclimatization rate in all culture systems. C. officinale plants showed the highest survival rate at 96.9%, which were regenerated in the TIS. TIS bioreactor culture, thus, provides a convenient method that could be adopted for commercial in vitro propagation of chrysanthemum, strawberry and C. officinale plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
TIS optimized shoot length, leaf number and biomass versus semi-solid and liquid cultures.
- Taxon
- Chrysanthemum morifolium Ramat.
Chrysanthemum ×morifoliumt0 - Treatment
- TISe0
- Outcome
- Shoot length, a number of leaves per regenerated shoot, fresh, and dry biomass of plantletse0
- Direction
- optimale0
- Value
- a number of leavese0
- Comparator
- semi-solid and liquid immersion culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Shoot length, a number of leaves per regenerated shoot, fresh, and dry biomass of plantlets were optimal with the TIS compared to semi-solid and liquid immersion cultures”
t0“Chrysanthemum morifolium Ramat.”
Claim · model-written summary
TIS regenerated chrysanthemum leaf area was 2.87 cm2.
Verbatim
e0“The leaf area in cryshanthmum, strawberry, and C. afficinale were 2.87 cm2, 3.51 cm2, and 1.43 cm2, respectively, in the plants regenerated by TIS”
Claim · model-written summary
TIS regenerated strawberry leaf area was 3.51 cm2.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassae0 - Treatment
- TISe0
- Outcome
- leaf areae0
- Value
- 3.51 cm2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“The leaf area in cryshanthmum, strawberry, and C. afficinale were 2.87 cm2, 3.51 cm2, and 1.43 cm2, respectively, in the plants regenerated by TIS”
Claim · model-written summary
TIS regenerated C. afficinale leaf area was 1.43 cm2.
Verbatim
e0“The leaf area in cryshanthmum, strawberry, and C. afficinale were 2.87 cm2, 3.51 cm2, and 1.43 cm2, respectively, in the plants regenerated by TIS”
Claim · model-written summary
Strawberry photosynthetic pigments were highest under TIS.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- TIS bioreactor culturee0
- Outcome
- photosynthetic pigmentse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The photosynthetic pigments were highest in strawberry plants grown in TIS bioreactor culture”
Claim · model-written summary
Strawberry pigments did not differ significantly between semi-solid and liquid culture.
- Taxon
- strawberryFragaria ×ananassat0
- Outcome
- photosynthetic pigmentst1
- Direction
- no significant differencee0
- Comparator
- semi-solid and liquid culturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no significant difference between semi-solid and liquid culture”
t0“strawberry plants showed a 100% acclimatization rate”
t1“The photosynthetic pigments”
Claim · model-written summary
C. officinale photosynthetic pigments were highest in semi-solid culture.
- Taxon
- C. officinaleConioselinum officinalee0
- Treatment
- semi-solid culturee0
- Outcome
- photosynthetic pigmentst1
- Direction
- higheste0
Not reportedComparator
Verbatim
e0“the highest values were obtained in C. officinale maintained in semi-solid culture”
t1“The photosynthetic pigments”
Claim · model-written summary
Chrysanthemum acclimatization reached 100% in all culture systems.
- Taxon
- chrysanthemum
.jpeg?width=60)
Chrysanthemume0 - Outcome
- acclimatization ratee0
- Value
- 100%e0
- Qualifier
- all culture systemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpeg?width=500)
Verbatim
e0“The chrysanthemum and strawberry plants showed a 100% acclimatization rate in all culture systems”
Claim · model-written summary
Strawberry acclimatization reached 100% in all culture systems.
- Taxon
- strawberryFragaria ×ananassae0
- Outcome
- acclimatization ratee0
- Value
- 100%e0
- Qualifier
- all culture systemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The chrysanthemum and strawberry plants showed a 100% acclimatization rate in all culture systems”
Claim · model-written summary
C. officinale survival peaked at 96.9% with TIS regeneration.
Verbatim
e0“C. officinale plants showed the highest survival rate at 96.9%, which were regenerated in the TIS”
Claim · model-written summary
Authors recommend TIS for commercial propagation of chrysanthemum, strawberry and C. officinale.
- Taxon
- chrysanthemumChrysanthemume0
- Treatment
- TIS bioreactor culturee0
- Outcome
- commercial in vitro propagatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TIS bioreactor culture, thus, provides a convenient method that could be adopted for commercial in vitro propagation of chrysanthemum, strawberry and C. officinale plants”
The same questions in other papers
application · physiology: photosynthesis · Fragaria x ananassa (strawberry)Positive and negative results
8 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.