Abstract
Owing to the large genetic diversity of barley and its resilience under harsh environments, this crop is of great value for agroecological transition and the need for reduction of nitrogen (N) fertilizers inputs. In the present work, we investigated the diversity of a North African barley genotype collection in terms of growth under limiting N (LN) or ample N (HN) supply and in terms of physiological traits including amino acid content in young seedlings. We identified a Moroccan variety, Laanaceur, accumulating five times more lysine in its leaves than the others under both N nutritional regimes. Physiological characterization of the barley collection showed the genetic diversity of barley adaptation strategies to LN and highlighted a genotype x environment interaction. In all genotypes, N limitation resulted in global biomass reduction, an increase in C concentration, and a higher resource allocation to the roots, indicating that this organ undergoes important adaptive metabolic activity. The most important diversity concerned leaf nitrogen use efficiency (LNUE), root nitrogen use efficiency (RNUE), root nitrogen uptake efficiency (RNUpE), and leaf nitrogen uptake efficiency (LNUpE). Using LNUE as a target trait reflecting barley capacity to deal with N limitation, this trait was positively correlated with plant nitrogen uptake efficiency (PNUpE) and RNUpE. Based on the LNUE trait, we determined three classes showing high, moderate, or low tolerance to N limitation. The transcriptomic approach showed that signaling, ionic transport, immunity, and stress response were the major functions affected by N supply. A candidate gene encoding the HvNRT2.10 transporter was commonly up-regulated under LN in the three barley genotypes investigated. Genes encoding key enzymes required for lysine biosynthesis in plants, dihydrodipicolinate synthase (DHPS) and the catabolic enzyme, the bifunctional Lys-ketoglutarate reductase/saccharopine dehydrogenase are up-regulated in Laanaceur and likely account for a hyperaccumulation of lysine in this genotype. Our work provides key physiological markers of North African barley response to low N availability in the early developmental stages.
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Claims 13
Claim · model-written summary
Laanaceur leaves accumulated five times more lysine than other varieties under both nitrogen regimes.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Material
- Laanaceure0
- Organ / stage
- leavese0
- Outcome
- lysinee0
- Direction
- moree0
- Value
- five timese0
- Qualifier
- both N nutritional regimese0
- Comparator
- the otherse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“We identified a Moroccan variety, Laanaceur, accumulating five times more lysine in its leaves than the others under both N nutritional regimes.”
t0“barley”
Claim · model-written summary
Barley adaptation to limiting nitrogen showed a genotype by environment interaction.
- Taxon
- barleyHordeum vulgaree0
- Material
- barley collectione0
- Outcome
- genotype x environment interactione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Physiological characterization of the barley collection showed the genetic diversity of barley adaptation strategies to LN and highlighted a genotype x environment interaction.”
Claim · model-written summary
Nitrogen limitation reduced biomass in all barley genotypes.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- N limitatione0
- Outcome
- biomasse0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In all genotypes, N limitation resulted in global biomass reduction, an increase in C concentration, and a higher resource allocation to the roots”
t0“barley”
Claim · model-written summary
Nitrogen limitation increased carbon concentration in all barley genotypes.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- N limitatione0
- Outcome
- C concentratione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In all genotypes, N limitation resulted in global biomass reduction, an increase in C concentration, and a higher resource allocation to the roots”
t0“barley”
Claim · model-written summary
Nitrogen limitation increased allocation to barley roots.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- N limitatione0
- Outcome
- resource allocation to the rootse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In all genotypes, N limitation resulted in global biomass reduction, an increase in C concentration, and a higher resource allocation to the roots”
t0“barley”
Claim · model-written summary
The greatest genotypic diversity concerned four leaf and root nitrogen use and uptake efficiency traits.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- leaf nitrogen use efficiency (LNUE), root nitrogen use efficiency (RNUE), root nitrogen uptake efficiency (RNUpE), and leaf nitrogen uptake efficiency (LNUpE)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The most important diversity concerned leaf nitrogen use efficiency (LNUE), root nitrogen use efficiency (RNUE), root nitrogen uptake efficiency (RNUpE), and leaf nitrogen uptake efficiency (LNUpE).”
t0“barley”
Claim · model-written summary
Leaf nitrogen use efficiency positively correlated with plant nitrogen uptake efficiency.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- plant nitrogen uptake efficiency (PNUpE)e0
- Direction
- positively correlatede0
- Comparator
- LNUEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using LNUE as a target trait reflecting barley capacity to deal with N limitation, this trait was positively correlated with plant nitrogen uptake efficiency (PNUpE) and RNUpE.”
t0“barley”
Claim · model-written summary
Leaf nitrogen use efficiency positively correlated with root nitrogen use efficiency.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- RNUpEe0
- Direction
- positively correlatede0
- Comparator
- LNUEe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Using LNUE as a target trait reflecting barley capacity to deal with N limitation, this trait was positively correlated with plant nitrogen uptake efficiency (PNUpE) and RNUpE.”
t0“barley”
Claim · model-written summary
The authors classified barley genotypes as high, moderate or low in tolerance to nitrogen limitation.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- tolerance to N limitatione0
- Value
- three classese0
- Qualifier
- LNUE traite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Based on the LNUE trait, we determined three classes showing high, moderate, or low tolerance to N limitation.”
t0“barley”
Claim · model-written summary
Nitrogen supply affected signaling, ionic transport, immunity and stress-response functions.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- N supplye0
- Outcome
- signaling, ionic transport, immunity, and stress responsee0
- Direction
- affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptomic approach showed that signaling, ionic transport, immunity, and stress response were the major functions affected by N supply.”
t0“barley”
Claim · model-written summary
HvNRT2.10 was upregulated under low nitrogen in three barley genotypes.
- Taxon
- barleyHordeum vulgaree0
- Treatment
- LNe0
- Gene
- HvNRT2.10e0
- Outcome
- HvNRT2.10 transportere0
- Direction
- up-regulatede0
- Value
- threee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A candidate gene encoding the HvNRT2.10 transporter was commonly up-regulated under LN in the three barley genotypes investigated.”
Claim · model-written summary
Lysine biosynthesis and catabolism enzyme genes were upregulated in Laanaceur.
- Taxon
- barleyHordeum vulgaret0
- Material
- Laanaceure0
- Gene
- dihydrodipicolinate synthase (DHPS)e0
- Outcome
- Genes encoding key enzymes required for lysine biosynthesise0
- Direction
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genes encoding key enzymes required for lysine biosynthesis in plants, dihydrodipicolinate synthase (DHPS) and the catabolic enzyme, the bifunctional Lys-ketoglutarate reductase/saccharopine dehydrogenase are up-regulated in Laanaceur”
t0“barley”
Claim · model-written summary
The authors infer that upregulated lysine metabolic genes likely explain Laanaceur lysine accumulation.
- Taxon
- barleyHordeum vulgaret0
- Material
- Laanaceure0
- Outcome
- hyperaccumulation of lysinee0
- Qualifier
- up-regulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“are up-regulated in Laanaceur and likely account for a hyperaccumulation of lysine in this genotype.”
t0“barley”
The same questions in other papers
nitrogen · growth · Hordeum vulgare (barley)Results differ
65 papers in the same direction as this paper · 42 in the opposite direction · 2 reporting no difference from an alternative
This paper is about lacking the nutrient, so it counts the other way round here.
- negative
- positive
- no effect
106 papers more in this group
See all 110 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.