Abstract
Phosphatidylcholine (PC) is a major class of phospholipids that are essential for post-embryonic growth in plants. In Arabidopsis, three copies of the phospho-base N-methyltransferase, PMT1, PMT2, and PMT3, are known to account for PC biosynthesis because the triple-knockout mutant is devoid of biosynthesis and shows lethality in post-embryonic but not embryonic growth. Arabidopsis also contains a distinct phospholipid N-methyltransferase (PLMT) that is homologous with yeast and animal PLMT that methylates phospholipids to produce PC. However, the knockout mutant of PLMT does not show morphological phenotypes or decreased PC content, so the role of PLMT remains unclear. Here, we show that PLMT is ubiquitously expressed in different organs and localized at the endoplasmic reticulum, where PC is produced. Overexpression of PLMT in planta increased the content of phospholipids including PC and affected vegetative but not reproductive growth. Although silique lengths were shorter, pollen remained viable and mature seeds were produced. Intriguingly, seed triacylglycerol content was increased with altered fatty acid composition. We conclude that PLMT might be a functional enzyme in PC biosynthesis and play an organ-specific role in developing seeds, where rapid accumulation of triacylglycerol dominates the entire glycerolipid metabolic flux.
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Claims 8
Claim · model-written summary
PLMT was expressed across organs and localized to the endoplasmic reticulum.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- PLMTe0
- Outcome
- ubiquitously expressed in different organs and localized at the endoplasmic reticulume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PLMT is ubiquitously expressed in different organs and localized at the endoplasmic reticulum, where PC is produced.”
t0“Arabidopsis”
Claim · model-written summary
PLMT overexpression increased phospholipids including PC.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Overexpression of PLMTe0
- Gene
- PLMTe0
- Outcome
- content of phospholipids including PCe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PLMT in planta increased the content of phospholipids including PC and affected vegetative but not reproductive growth.”
t0“Arabidopsis”
Claim · model-written summary
PLMT overexpression affected vegetative but not reproductive growth.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Overexpression of PLMTe0
- Gene
- PLMTe0
- Outcome
- vegetative but not reproductive growthe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of PLMT in planta increased the content of phospholipids including PC and affected vegetative but not reproductive growth.”
t0“Arabidopsis”
Claim · model-written summary
Siliques were shorter, whereas pollen remained viable and mature seeds were produced.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- silique lengthse0
- Direction
- shortere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although silique lengths were shorter, pollen remained viable and mature seeds were produced.”
t0“Arabidopsis”
Claim · model-written summary
Pollen remained viable and mature seeds were produced.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- pollen remained viable and mature seeds were producede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Although silique lengths were shorter, pollen remained viable and mature seeds were produced.”
t0“Arabidopsis”
Claim · model-written summary
Seed triacylglycerol content increased.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- seede0
- Outcome
- triacylglycerol contente0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed triacylglycerol content was increased with altered fatty acid composition.”
t0“Arabidopsis”
Claim · model-written summary
Seed fatty acid composition was altered.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- seede0
- Outcome
- fatty acid compositione0
- Direction
- alterede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seed triacylglycerol content was increased with altered fatty acid composition.”
t0“Arabidopsis”
Claim · model-written summary
The authors suggest PLMT may function in PC biosynthesis and have an organ-specific role in developing seeds.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- developing seedse0
- Gene
- PLMTe0
- Outcome
- PC biosynthesise0
- Qualifier
- organ-specific rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PLMT might be a functional enzyme in PC biosynthesis and play an organ-specific role in developing seeds”
t0“Arabidopsis”