Abstract
Plant MAPK cascade performs a critical role in the regulation of plant immunity and disease resistance. Although the function of MAPK cascade in immunity regulation is partially conserved between different species, the mechanism varies in different host and pathogen combinations. To date, the MAPK cascade function of woody plants in the regulation of disease resistance has seldom been reported. Here, we present evidence to show that apple MdMAPKKK1 performed an important role in the regulation of apple resistance to Botryosphaeria dothidea, the causal agent of apple ring rot. B. dothidea infection leads to enhanced MdMAPKKK1 expression and MAPK cascade activation, indicating that the MAPK cascade is involved in the defense against B. dothidea. MdMAPKKK1 overexpression-induced pathogen-independent cell death. MdMAPKKK1 silencing decreases the resistance of apple calli and fruits to B. dothidea. Further analysis indicates that MdMAPKKK1 can bind MdBSK1 and is likely phosphorylated by it. The MdBSK1-mediated phosphorylation of MdMAPKKK1 is important for resistance to B. dothidea. These results collectively indicate that apple resistance to B. dothidea is regulated by the interaction between MAPKKK1 and MdBSK1.
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Claims 8
Claim · model-written summary
MdMAPKKK1 regulates apple resistance to B. dothidea.
- Taxon
- apple
Malus domesticae0 - Interactor
- Botryosphaeria dothidea
_Cesati_%26_DeNotaris_661320.jpg?width=60)
Botryosphaeria dothideae0pathogen - Gene
- MdMAPKKK1e0
- Outcome
- apple resistance to Botryosphaeria dothideae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Cesati_%26_DeNotaris_661320.jpg?width=500)
Verbatim
e0“apple MdMAPKKK1 performed an important role in the regulation of apple resistance to Botryosphaeria dothidea”
Claim · model-written summary
B. dothidea infection increased MdMAPKKK1 expression.
- Taxon
- appleMalus domesticat0
- Treatment
- B. dothidea infectione0
- Interactor
- B. dothideaBotryosphaeria dothideae0pathogen
- Gene
- MdMAPKKK1e0
- Outcome
- MdMAPKKK1 expressione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. dothidea infection leads to enhanced MdMAPKKK1 expression and MAPK cascade activation”
t0“Apple”
Claim · model-written summary
B. dothidea infection activated the MAPK cascade.
- Taxon
- appleMalus domesticat0
- Treatment
- B. dothidea infectione0
- Interactor
- B. dothideaBotryosphaeria dothideae0pathogen
- Outcome
- MAPK cascade activatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B. dothidea infection leads to enhanced MdMAPKKK1 expression and MAPK cascade activation”
t0“Apple”
Claim · model-written summary
MdMAPKKK1 overexpression induced cell death without pathogen infection.
- Taxon
- AppleMalus domesticat0
- Treatment
- MdMAPKKK1 overexpressione0
- Gene
- MdMAPKKK1e0
- Outcome
- pathogen-independent cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdMAPKKK1 overexpression-induced pathogen-independent cell death.”
t0“Apple”
Claim · model-written summary
MdMAPKKK1 silencing reduced resistance of apple calli and fruits to B. dothidea.
- Taxon
- appleMalus domesticae0
- Treatment
- MdMAPKKK1 silencinge0
- Interactor
- B. dothideaBotryosphaeria dothideae0pathogen
- Gene
- MdMAPKKK1e0
- Outcome
- resistance of apple calli and fruits to B. dothideae0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdMAPKKK1 silencing decreases the resistance of apple calli and fruits to B. dothidea.”
Claim · model-written summary
MdMAPKKK1 binds MdBSK1.
- Taxon
- AppleMalus domesticat0
- Gene
- MdMAPKKK1e0
- Outcome
- bind MdBSK1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdMAPKKK1 can bind MdBSK1 and is likely phosphorylated by it.”
t0“Apple”
Claim · model-written summary
MdMAPKKK1 is likely phosphorylated by MdBSK1.
- Taxon
- AppleMalus domesticat0
- Gene
- MdBSK1e0
- Outcome
- likely phosphorylated by ite0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdMAPKKK1 can bind MdBSK1 and is likely phosphorylated by it.”
t0“Apple”
Claim · model-written summary
MdBSK1-mediated phosphorylation of MdMAPKKK1 contributes to resistance to B. dothidea.
- Taxon
- AppleMalus domesticat0
- Interactor
- B. dothideaBotryosphaeria dothideae0pathogen
- Gene
- MdBSK1e0
- Outcome
- resistance to B. dothideae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The MdBSK1-mediated phosphorylation of MdMAPKKK1 is important for resistance to B. dothidea.”
t0“Apple”
The same questions in other papers
loss of function · Botryosphaeria dothidea · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.