Abstract
In plants, the vesicle fusion process plays a vital role in pathogen defence. However, the importance of the vesicle fusion process in apple ring rot has not been studied. Here, we isolated and characterised the apple syntaxin gene MdSYP121. Silencing the MdSYP121 gene in transgenic apple calli increased tolerance to Botryosphaeria dothidea infection; this increased tolerance was correlated with salicylic acid (SA) synthesis-related and signalling-related gene transcription. In contrast, overexpressing MdSYP121 in apple calli resulted in the opposite phenotypes. In addition, the results of RNA sequencing (RNA-Seq) and quantitative real-time PCR (qRT-PCR) assays suggested that MdSYP121 plays an important role in responses to oxidation-reduction reactions. Silencing MdSYP121 in apple calli enhanced the expression levels of reactive oxygen species (ROS)-related genes and the activity of ROS-related enzymes. The enhanced defence response status in MdSYP121-RNAi lines suggests that syntaxins are involved in the defence response to B. dothidea. More importantly, we showed that MdSYP121 forms a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex with MdSNAP33, and the complex may participate in regulating resistance to B. dothidea. In conclusion, by regulating the interaction of SA pathway and oxidation-reduction process, MdSYP121 can influence the pathogen infection process in apple.
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Claims 7
Claim · model-written summary
The apple syntaxin gene MdSYP121 was isolated and characterized.
- Taxon
- apple
Malus domesticae0 - Gene
- MdSYP121e0
- Outcome
- apple syntaxin gene MdSYP121e0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we isolated and characterised the apple syntaxin gene MdSYP121.”
Claim · model-written summary
Silencing MdSYP121 increased apple callus tolerance to B. dothidea infection.
- Taxon
- appleMalus domesticae0
- Treatment
- Silencing the MdSYP121 genee0
- Interactor
- Botryosphaeria dothidea
_Cesati_%26_DeNotaris_661320.jpg?width=60)
Botryosphaeria dothideae0pathogen - Gene
- MdSYP121e0
- Outcome
- tolerance to Botryosphaeria dothidea infectione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Cesati_%26_DeNotaris_661320.jpg?width=500)
Verbatim
e0“Silencing the MdSYP121 gene in transgenic apple calli increased tolerance to Botryosphaeria dothidea infection”
Claim · model-written summary
Increased tolerance after MdSYP121 silencing correlated with SA synthesis and signaling gene transcription.
- Gene
- salicylic acid (SA) synthesis-related and signalling-related genee0
- Outcome
- increased tolerancee0
- Direction
- correlated withe0
Not reportedTaxon
Verbatim
e0“this increased tolerance was correlated with salicylic acid (SA) synthesis-related and signalling-related gene transcription.”
Claim · model-written summary
MdSYP121 overexpression in apple calli produced phenotypes opposite to silencing.
- Taxon
- appleMalus domesticae0
- Treatment
- overexpressing MdSYP121e0
- Gene
- MdSYP121e0
- Outcome
- phenotypese0
- Direction
- oppositee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpressing MdSYP121 in apple calli resulted in the opposite phenotypes.”
Claim · model-written summary
MdSYP121 was implicated in responses to oxidation-reduction reactions.
Not reportedTaxonDirection
Verbatim
e0“MdSYP121 plays an important role in responses to oxidation-reduction reactions.”
Claim · model-written summary
Silencing MdSYP121 raised ROS-related gene expression and enzyme activity in apple calli.
- Taxon
- appleMalus domesticae0
- Treatment
- Silencing MdSYP121e0
- Gene
- MdSYP121e0
- Outcome
- expression levels of reactive oxygen species (ROS)-related genes and the activity of ROS-related enzymese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing MdSYP121 in apple calli enhanced the expression levels of reactive oxygen species (ROS)-related genes and the activity of ROS-related enzymes.”
Claim · model-written summary
MdSYP121 forms a SNARE complex with MdSNAP33.
Not reportedTaxonDirection
Verbatim
e0“MdSYP121 forms a soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex with MdSNAP33”
The same questions in other papers
loss of function · physiology: oxidative · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
loss of function · Botryosphaeria dothidea · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- negative
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.