Abstract
The vesicular transport system is important for substance transport in plants. In recent years, the regulatory relationship between the vesicular transport system and plant disease resistance has received widespread attention; however, the underlying mechanism remains unclear. MdSYP121 is a key protein in the vesicular transport system. The overexpression of MdSYP121 decreased the B. dothidea resistance of apple, while silencing MdSYP121 resulted in the opposite phenotype. A metabolome and transcriptome dataset analysis showed that MdSYP121 regulated apple disease resistance by significantly affecting sugar metabolism. HPLC results showed that the levels of many soluble sugars were significantly higher in the MdSYP121-OE calli. Furthermore, the expression levels of genes related to sugar transport were significantly higher in the MdSYP121-OE calli after B. dothidea inoculation. In addition, the relationships between the MdSYP121 expression level, the soluble sugar content, and apple resistance to B. dothidea were verified in an F1 population derived from a cross between ‘Golden Delicious’ and ‘Fuji Nagafu No. 2’. In conclusion, these results suggested that MdSYP121 negatively regulated apple resistance to B. dothidea by influencing the soluble sugar content. These technologies and methods allow us to investigate the molecular mechanism of the vesicular transport system regulating apple resistance to B. dothidea.
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Claims 6
Claim · model-written summary
MdSYP121 overexpression reduced apple resistance to B. dothidea.
- Taxon
- apple
Malus domesticae0 - Treatment
- overexpression of MdSYP121e0
- Interactor
- B. dothidea
_Cesati_%26_DeNotaris_661320.jpg?width=60)
Botryosphaeria dothideae0pathogen - Gene
- MdSYP121e0
- Outcome
- B. dothidea resistancee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Cesati_%26_DeNotaris_661320.jpg?width=500)
Verbatim
e0“The overexpression of MdSYP121 decreased the B. dothidea resistance of apple, while silencing MdSYP121 resulted in the opposite phenotype.”
Claim · model-written summary
Silencing MdSYP121 increased apple resistance to B. dothidea.
- Taxon
- appleMalus domesticae0
- Treatment
- silencing MdSYP121e0
- Interactor
- B. dothideaBotryosphaeria dothideae0pathogen
- Gene
- MdSYP121e0
- Outcome
- B. dothidea resistancee0
- Direction
- opposite phenotypee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of MdSYP121 decreased the B. dothidea resistance of apple, while silencing MdSYP121 resulted in the opposite phenotype.”
Claim · model-written summary
Dataset analysis linked MdSYP121 regulation of disease resistance to sugar metabolism.
- Taxon
- appleMalus domesticae0
- Gene
- MdSYP121e0
- Outcome
- apple disease resistancee0
- Qualifier
- sugar metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A metabolome and transcriptome dataset analysis showed that MdSYP121 regulated apple disease resistance by significantly affecting sugar metabolism.”
Claim · model-written summary
Many soluble sugars were higher in MdSYP121 overexpression calli.
Not reportedTaxon
Verbatim
e0“HPLC results showed that the levels of many soluble sugars were significantly higher in the MdSYP121-OE calli.”
Claim · model-written summary
Sugar transport gene expression was higher in MdSYP121 overexpression calli after pathogen inoculation.
- Material
- MdSYP121-OE callie0
- Interactor
- B. dothideaBotryosphaeria dothideae0pathogen
- Outcome
- expression levels of genes related to sugar transporte0
- Direction
- highere0
Not reportedTaxon
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, the expression levels of genes related to sugar transport were significantly higher in the MdSYP121-OE calli after B. dothidea inoculation.”
Claim · model-written summary
An F1 population verified relationships among MdSYP121 expression, soluble sugar content and pathogen resistance.
- Taxon
- appleMalus domesticae0
- Material
- F1 populatione0
- Interactor
- B. dothideaBotryosphaeria dothideae0pathogen
- Gene
- MdSYP121e0
- Outcome
- apple resistance to B. dothideae0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the relationships between the MdSYP121 expression level, the soluble sugar content, and apple resistance to B. dothidea were verified in an F1 population derived from a cross between ‘Golden Delicious’ and ‘Fuji Nagafu No. 2’.”
The same questions in other papers
loss of function · Botryosphaeria dothidea · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.