Abstract
Drought stress significantly impedes apple growth, development, and yield, leading to substantial economic losses within the global apple industry. Malus prunifolia ( Mp ), a commonly utilized apple rootstock, has shown promise in augmenting cultivated apple resistance to abiotic stress. Although Alfin-like (ALs) proteins have demonstrated pivotal roles in dicotyledonous plants' response to abiotic stresses, knowledge about AL genes in apple rootstocks is limited, and their functions remain largely elusive. In this study, we identified and characterized 10 MpAL gene members in the apple rootstock genome, confirming their localization within the nucleus. Our investigation revealed the significant regulation of MpALs ' expression under drought and abscisic acid (ABA) stresses in M. prunifolia . In this study, one of the members, MpAL1 , was selected for further exploration in Arabidopsis and apple to explore its potential function in response to drought and ABA stresses. The results showed that overexpression- MpAL1 transgenic apple calli grew significantly better than WT and MpAL1 -RNAi lines, which regulates the accumulation of H 2 O 2 and O 2 - levels. Additionally, transgenic Arabidopsis plants overexpressing MpAL1 exhibited positively regulating anti-oxidant enzymes activities under stress treatments. Further study showed that silencing MpAL1 in apple plants showed obvious chlorosis in leaves, and accumulation of reactive oxygen species under drought stress. Moreover, our detailed analysis established that MpAL1 regulates several drought and ABA-responsive genes, exerting an influence on their expression in transgenic apple. Collectively, our findings identify MpAL1 as a positive regulator that increases drought stress in apple, shedding light on its potential significance in bolstering drought resistance in this fruit crop.
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Claims 9
Claim · model-written summary
Ten MpAL gene members were identified and characterized in the apple rootstock genome.
- Taxon
- Malus prunifolia
Malus prunifoliat0 - Gene
- MpALe1
- Outcome
- MpAL gene memberse1
- Value
- 10e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we identified and characterized 10 MpAL gene members in the apple rootstock genome”
t0“in Malus prunifolia”
Claim · model-written summary
MpALs expression was significantly regulated under drought and ABA stresses in M. prunifolia.
- Taxon
- M. prunifoliaMalus prunifoliae2
- Treatment
- drought and abscisic acid (ABA) stressese2
- Gene
- MpALse2
- Outcome
- expressione2
- Direction
- significant regulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Our investigation revealed the significant regulation of MpALs ' expression under drought and abscisic acid (ABA) stresses in M. prunifolia”
t0“in Malus prunifolia”
Claim · model-written summary
MpAL1 overexpressing apple calli grew significantly better than wild-type and MpAL1-RNAi lines.
- Taxon
- apple
Malus domesticae3 - Treatment
- overexpression- MpAL1e3
- Gene
- MpAL1e3
- Outcome
- callie3
- Direction
- grew significantly bettere3
- Comparator
- WT and MpAL1 -RNAi linese3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“overexpression- MpAL1 transgenic apple calli grew significantly better than WT and MpAL1 -RNAi lines”
t0“in Malus prunifolia”
Claim · model-written summary
MpAL1 regulates the accumulation of H2O2 and O2- levels in apple.
- Taxon
- Malus prunifoliaMalus prunifoliat0
- Gene
- MpAL1e3
- Outcome
- accumulatione4
- Qualifier
- H 2 O 2 and O 2 - levelse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“which regulates the accumulation of H 2 O 2 and O 2 - levels”
e3“overexpression- MpAL1 transgenic apple calli grew significantly better than WT and MpAL1 -RNAi lines”
t0“in Malus prunifolia”
Claim · model-written summary
Transgenic Arabidopsis overexpressing MpAL1 positively regulated antioxidant enzyme activities under stress.
- Taxon
- Arabidopsis
Arabidopsise5 - Treatment
- overexpressing MpAL1e5
- Gene
- MpAL1e5
- Outcome
- anti-oxidant enzymes activitiese5
- Direction
- positively regulatinge5
- Qualifier
- under stress treatmentse5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“transgenic Arabidopsis plants overexpressing MpAL1 exhibited positively regulating anti-oxidant enzymes activities under stress treatments”
t0“in Malus prunifolia”
Claim · model-written summary
Silencing MpAL1 in apple plants caused obvious chlorosis in leaves.
- Taxon
- appleMalus domesticae6
- Treatment
- silencing MpAL1e6
- Outcome
- chlorosis in leavese6
- Direction
- showed obviouse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“silencing MpAL1 in apple plants showed obvious chlorosis in leaves, and accumulation of reactive oxygen species under drought stress”
t0“in Malus prunifolia”
Claim · model-written summary
Silencing MpAL1 increased reactive oxygen species accumulation in apple under drought stress.
- Taxon
- appleMalus domesticae6
- Treatment
- silencing MpAL1e6
- Outcome
- reactive oxygen speciese6
- Direction
- accumulatione6
- Qualifier
- under drought stresse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“silencing MpAL1 in apple plants showed obvious chlorosis in leaves, and accumulation of reactive oxygen species under drought stress”
t0“in Malus prunifolia”
Claim · model-written summary
MpAL1 regulates the expression of several drought- and ABA-responsive genes in transgenic apple.
- Taxon
- appleMalus domesticae7
- Gene
- MpAL1e7
- Outcome
- expressione7
- Qualifier
- drought and ABA-responsive genese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“MpAL1 regulates several drought and ABA-responsive genes, exerting an influence on their expression in transgenic apple”
t0“in Malus prunifolia”
Claim · model-written summary
MpAL1 is a positive regulator that increases drought stress resistance in apple.
- Taxon
- appleMalus domesticae0
- Gene
- MpAL1e0
- Outcome
- drought stresse0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“our findings identify MpAL1 as a positive regulator that increases drought stress in apple”
t0“in Malus prunifolia”
The same questions in other papers
loss of function · physiology: oxidative · Malus domestica (apple)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
loss of function · physiology: pigments · Malus domestica (apple)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.