Abstract
Chloride is an essential nutrient for plants, but high concentrations can be harmful. Silicon ameliorates both abiotic and biotic stresses in plants, but it is unknown if it can prevent cellular increase of chloride. Therefore, we investigated the influx of Cl− ions in two wheat cultivars different in salt sensitivity, by epifluorescence microscopy and a highly Cl−-sensitive dye, MQAE, N-[ethoxycarbonylmethyl]-6-methoxy-quinolinium bromide, in absence and presence of potassium silicate, K2SiO3. The Cl−-influx was higher in the salt-sensitive cv. Vinjett, than in the salt-tolerant cv. S-24, and silicate pre-treatment of protoplasts inhibited the Cl−-influx in both cultivars, but more in the sensitive cv. Vinjett. To investigate if the Cl−-transporters TaCLC1 and TaNPF2.4/2.5 are affected by silicate, expression analyses by RT-qPCR were undertaken of TaCLC1 and TaNPF 2.4/2.5 transcripts in the absence and presence of 100 mM NaCl, with and without the presence of K2SiO3. The results show that both transporter genes were expressed in roots and shoots of wheat seedlings, but their expressions were differently affected by silicate. The TaNPF2.4/2.5 expression in leaves was markedly depressed by silicate. These findings demonstrate that less chloride accumulates in the cytosol of leaf mesophyll by Si treatment and increases salt tolerance.
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Claims 8
Claim · model-written summary
Salt-sensitive Vinjett had higher chloride influx than salt-tolerant S-24.
- Taxon
- Wheat
Triticum aestivumt0 - Material
- cv. Vinjette0
- Outcome
- Cl−-influxe0
- Direction
- highere0
- Comparator
- cv. S-24e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Cl−-influx was higher in the salt-sensitive cv. Vinjett, than in the salt-tolerant cv. S-24”
t0“Wheat”
Claim · model-written summary
Silicate pretreatment inhibited chloride influx in both wheat cultivars.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- protoplastse0
- Treatment
- silicate pre-treatmente0
- Outcome
- Cl−-influxe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silicate pre-treatment of protoplasts inhibited the Cl−-influx in both cultivars, but more in the sensitive cv. Vinjett”
t0“Wheat”
Claim · model-written summary
Silicate inhibition of chloride influx was stronger in Vinjett.
- Taxon
- WheatTriticum aestivumt0
- Material
- cv. Vinjette0
- Treatment
- silicate pre-treatmente0
- Outcome
- Cl−-influxe0
- Direction
- moree0
- Comparator
- both cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silicate pre-treatment of protoplasts inhibited the Cl−-influx in both cultivars, but more in the sensitive cv. Vinjett”
t0“Wheat”
Claim · model-written summary
Both transporter genes were expressed in wheat roots and shoots.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaCLC1 and TaNPF2.4/2.5e1
- Outcome
- expressed in roots and shootse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both transporter genes were expressed in roots and shoots of wheat seedlings”
e1“TaCLC1 and TaNPF2.4/2.5 are affected by silicate”
Claim · model-written summary
Silicate affected expression of the two transporters differently.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- silicatee0
- Gene
- TaCLC1 and TaNPF2.4/2.5e1
- Outcome
- expressionse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their expressions were differently affected by silicate”
e1“TaCLC1 and TaNPF2.4/2.5 are affected by silicate”
t0“Wheat”
Claim · model-written summary
Silicate markedly depressed leaf TaNPF2.4/2.5 expression.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- silicatee0
- Gene
- TaNPF2.4/2.5e0
- Outcome
- expression in leavese0
- Direction
- depressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The TaNPF2.4/2.5 expression in leaves was markedly depressed by silicate”
t0“Wheat”
Claim · model-written summary
The authors conclude silicon treatment reduces cytosolic chloride accumulation in leaf mesophyll.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Si treatmente0
- Outcome
- chloride accumulates in the cytosol of leaf mesophylle0
- Direction
- lesse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“less chloride accumulates in the cytosol of leaf mesophyll by Si treatment”
t0“Wheat”
Claim · model-written summary
The authors conclude silicon treatment increases salt tolerance.
- Taxon
- WheatTriticum aestivumt0
- Treatment
- Si treatmente0
- Outcome
- salt tolerancee0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“by Si treatment and increases salt tolerance”
t0“Wheat”
The same questions in other papers
silicon atom · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results
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- negative
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- positive
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.