Abstract
Upland cotton (Gossypium hirsutum) is a salt-tolerant crop that can withstand high salinity levels without showing signs of harm to the plant. However, the plant is more prone to salinity stress at the germination stage and a poor germination as well as poor crop stand lead to a weak productivity. It is possible to obtain a comprehensive picture of the cotton seedling germination and establishment against salt stress by examining dynamic changes in the transcriptomic and metabolomic profiles. The reported study employed a pretreatment of cotton seeds by soaking them in 0.2% Magnesium Sulphate (MgSO4) solution at room temperature for 4, 8, and 12 h. The analysis of variance based on the studied traits emergence rate, above and underground plant parts’ fresh weight measured, displayed significant differences of the three treatments compared with the control. A total of 28,801 and 264 differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were discovered to code for biological processes such as response to salt stress, cellular response to salt stress, abscisic acid receptor PYR/PYL, regulation of seed growth and germination, and auxin-activated signaling pathways. A large amount of ethylene-responsive transcription factors (ERF) was identified (1235) as differentially expressed, followed by bHLH (252), WRKY (96), MYB (202), GATA (81), RABA (64), DIVARICATA (28), and MADs-box (26) in treated seedling samples. Functional enrichment analysis revealed the significant roles in the hormones and signal transduction, carbohydrates metabolism, and biosynthesis of amino acids, promoting salt stress tolerance. Our results indicated positive effects of MgSO4 at 4 h treatment on seedling germination and growth, seemingly by activating certain growth-regulating enzymes (auxins, gibberellins, jasmonates, abscisic acid, and salicylic acid) and metabolites (phenolic acids, flavonoids, and akaloids). Such pretreatment of MgSO4 on seeds would be beneficial in future cotton management under saline conditions to enhance good crop stand and productivity.
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Claims 8
Claim · model-written summary
Three MgSO4 soaking durations differed significantly from control for emergence and fresh weight traits.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- three treatmentse0
- Outcome
- emergence rate, above and underground plant parts’ fresh weighte0
- Comparator
- controle0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The analysis of variance based on the studied traits emergence rate, above and underground plant parts’ fresh weight measured, displayed significant differences of the three treatments compared with the control.”
t0“cotton”
Claim · model-written summary
The analysis identified 28,801 differentially expressed genes.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 28,801e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 28,801 and 264 differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were discovered”
t0“cotton”
Claim · model-written summary
The analysis identified 264 differentially accumulated metabolites.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- differentially accumulated metabolites (DAMs)e0
- Value
- 264e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 28,801 and 264 differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were discovered”
t0“cotton”
Claim · model-written summary
Treated seedlings had 1235 differentially expressed ERF transcription factors.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- ethylene-responsive transcription factors (ERF)e0
- Outcome
- differentially expressede0
- Value
- 1235e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A large amount of ethylene-responsive transcription factors (ERF) was identified (1235) as differentially expressed”
t0“cotton”
Claim · model-written summary
Treated seedlings also showed differentially expressed bHLH, WRKY, MYB, GATA, RABA, DIVARICATA, and MADs-box factors.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- bHLHe0
- Value
- 252e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“followed by bHLH (252), WRKY (96), MYB (202), GATA (81), RABA (64), DIVARICATA (28), and MADs-box (26) in treated seedling samples”
t0“cotton”
Claim · model-written summary
Enrichment implicated hormones, signal transduction, carbohydrate metabolism, and amino acid biosynthesis in salt tolerance.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- salt stress tolerancee0
- Qualifier
- hormones and signal transduction, carbohydrates metabolism, and biosynthesis of amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Functional enrichment analysis revealed the significant roles in the hormones and signal transduction, carbohydrates metabolism, and biosynthesis of amino acids, promoting salt stress tolerance.”
t0“cotton”
Claim · model-written summary
Four-hour MgSO4 pretreatment positively affected seedling germination and growth.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- MgSO4 at 4 h treatmente0
- Outcome
- seedling germination and growthe0
- Direction
- positive effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Our results indicated positive effects of MgSO4 at 4 h treatment on seedling germination and growth”
t0“cotton”
Claim · model-written summary
The authors recommend MgSO4 seed pretreatment for future cotton management under saline conditions.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- pretreatment of MgSO4 on seedse0
- Outcome
- good crop stand and productivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Such pretreatment of MgSO4 on seeds would be beneficial in future cotton management under saline conditions to enhance good crop stand and productivity.”
The same questions in other papers
magnesium sulfate · growth · Gossypium hirsutum (cotton)Results differ
7 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.