Abstract
Chlorophyll loss is a major problem during green vegetable storage. However, the molecular mechanism is still unclear. In this study, a 21 days of storage experiments showed chlorophyll content was higher in light-stored Chinese cabbage (Brassica chinensis L.) leaves than those in dark-stored samples. Transcriptome analyses were performed on these samples to determine the effects of light. Among 311 differentially expressed genes (DEGs), early light-induced protein 1 (ELIP1) was identified as the main control gene for chlorophyll synthesis. Tissues and subcellular localization indicated that ELIP1 was localized in the nucleus. Motifs structure analyses, chromatin immunoprecipitation (ChIP) assays, luciferase reporter assays, and overexpression experiments demonstrated that ELIP1 regulated the expressions of genomes uncoupled 4 (GUN4), Glutamyl-tRNA reductase family protein (HEMA1), and Mg-protoporphyrin IX methyltransferase (CHLM) by binding to G-box-like motifs and affected chlorophyll biosynthesis during the storage of Chinese cabbage. It is a possible common tetrapyrrole biosynthetic pathway for chlorophylls, hemes, and bilin pigments in photosynthetic organisms. Our research also revealed that white light can be used as a regulatory factor to improve the storage ability and extent shelf life of Chinese cabbage.
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Claims 7
Claim · model-written summary
Chlorophyll content was higher in light-stored Chinese cabbage leaves than in dark-stored leaves.
- Taxon
- Chinese cabbage (Brassica chinensis L.)
Brassica rapa subsp. pekinensise1 - Organ / stage
- leavese1
- Treatment
- light-storede1
- Outcome
- chlorophyll contente1
- Direction
- highere1
- Comparator
- dark-stored samplese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“chlorophyll content was higher in light-stored Chinese cabbage (Brassica chinensis L.) leaves than those in dark-stored samples”
Claim · model-written summary
ELIP1 was identified as the main control gene for chlorophyll synthesis among 311 differentially expressed genes in transcriptome analysis.
- Taxon
- Chinese cabbageBrassica rapa subsp. pekinensist0
- Gene
- ELIP1e2
- Outcome
- main control gene for chlorophyll synthesise2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Light regulates chlorophyll biosynthesis via ELIP1 during the storage of Chinese cabbage”
e2“Among 311 differentially expressed genes (DEGs), early light-induced protein 1 (ELIP1) was identified as the main control gene for chlorophyll synthesis”
Claim · model-written summary
ELIP1 is localized in the nucleus.
- Taxon
- Chinese cabbageBrassica rapa subsp. pekinensist0
- Gene
- ELIP1e3
- Outcome
- localized in the nucleuse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Light regulates chlorophyll biosynthesis via ELIP1 during the storage of Chinese cabbage”
e3“ELIP1 was localized in the nucleus”
Claim · model-written summary
ELIP1 regulates the expression of GUN4, HEMA1 and CHLM by binding to G-box-like motifs.
- Taxon
- Chinese cabbageBrassica rapa subsp. pekinensist0
- Gene
- genomes uncoupled 4 (GUN4), Glutamyl-tRNA reductase family protein (HEMA1), and Mg-protoporphyrin IX methyltransferase (CHLM)e4
- Outcome
- expressionse4
- Qualifier
- by binding to G-box-like motifse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Light regulates chlorophyll biosynthesis via ELIP1 during the storage of Chinese cabbage”
e4“ELIP1 regulated the expressions of genomes uncoupled 4 (GUN4), Glutamyl-tRNA reductase family protein (HEMA1), and Mg-protoporphyrin IX methyltransferase (CHLM) by binding to G-box-like motifs and affected chlorophyll biosynthesis during the storage of Chinese cabbage”
Claim · model-written summary
ELIP1 affects chlorophyll biosynthesis during storage of Chinese cabbage.
- Taxon
- Chinese cabbageBrassica rapa subsp. pekinensist0
- Gene
- ELIP1e4
- Outcome
- chlorophyll biosynthesise4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Light regulates chlorophyll biosynthesis via ELIP1 during the storage of Chinese cabbage”
e4“ELIP1 regulated the expressions of genomes uncoupled 4 (GUN4), Glutamyl-tRNA reductase family protein (HEMA1), and Mg-protoporphyrin IX methyltransferase (CHLM) by binding to G-box-like motifs and affected chlorophyll biosynthesis during the storage of Chinese cabbage”
Claim · model-written summary
The authors propose a possible common tetrapyrrole biosynthetic pathway for chlorophylls, hemes and bilin pigments.
- Taxon
- Chinese cabbageBrassica rapa subsp. pekinensist0
- Outcome
- common tetrapyrrole biosynthetic pathwaye5
- Qualifier
- chlorophylls, hemes, and bilin pigmentse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Light regulates chlorophyll biosynthesis via ELIP1 during the storage of Chinese cabbage”
e5“It is a possible common tetrapyrrole biosynthetic pathway for chlorophylls, hemes, and bilin pigments in photosynthetic organisms”
Claim · model-written summary
The authors recommend white light to improve storage ability and shelf life of Chinese cabbage.
- Taxon
- Chinese cabbageBrassica rapa subsp. pekinensist0
- Treatment
- white lighte6
- Outcome
- storage ability and extent shelf lifee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Light regulates chlorophyll biosynthesis via ELIP1 during the storage of Chinese cabbage”
e6“white light can be used as a regulatory factor to improve the storage ability and extent shelf life of Chinese cabbage”
The same questions in other papers
light · physiology: pigments · Brassica rapa (field mustard)Positive and negative results
11 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.