Abstract
Low temperatures are known to destroy cell membranes' structural integrity by affecting the remodeling of their phospholipids. Fruits stored at low temperature are prone to chilling injury, characterized by discoloration, absence of ripening, surface pitting, growth inhibition, flavor loss, decay, and wilting. Phosphatidic acid, a vital second-messenger lipid in plants, is known to accumulate in response to different kinds of stress stimuli. However, the regulatory mechanism of its production from the degradation of phospholipids remains poorly understood. We identified two cold-responsive NAC (NAM/ATAF1/CUC2) transcription factors from bananas, namely, MaNAC25 and MaNAC28, which negatively regulated cold tolerance in banana fruits by upregulating the expression of phospholipid degradation genes in banana fruits. Furthermore, MaNAC25 and MaNAC28 formed a positive feedback loop to induce phospholipid degradation and produce phosphatidic acid. In contrast, ethylene directly inhibited the degradation of phospholipids in banana and transgenic tomato fruits. In addition, ethylene reduced the activity of MaNAC25 and MaNAC28, thereby inhibiting phospholipid degradation. To conclude, NAC-mediated membrane lipid remodeling negatively regulates the cold tolerance of banana and transgenic tomato fruits.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 7
Claim · model-written summary
MaNAC25 and MaNAC28 were identified as cold responsive banana transcription factors.
Verbatim
e0“We identified two cold-responsive NAC (NAM/ATAF1/CUC2) transcription factors from bananas, namely, MaNAC25 and MaNAC28”
Claim · model-written summary
MaNAC25 and MaNAC28 negatively regulated cold tolerance in banana fruit by upregulating phospholipid degradation genes.
Verbatim
e0“MaNAC25 and MaNAC28, which negatively regulated cold tolerance in banana fruits by upregulating the expression of phospholipid degradation genes in banana fruits”
Claim · model-written summary
MaNAC25 and MaNAC28 formed a positive feedback loop that induced phospholipid degradation.
Verbatim
e0“MaNAC25 and MaNAC28 formed a positive feedback loop to induce phospholipid degradation and produce phosphatidic acid”
e1“cold tolerance in banana fruits”
Claim · model-written summary
The NAC feedback loop produced phosphatidic acid.
Verbatim
e0“MaNAC25 and MaNAC28 formed a positive feedback loop to induce phospholipid degradation and produce phosphatidic acid”
e1“cold tolerance in banana fruits”
Claim · model-written summary
Ethylene directly inhibited phospholipid degradation in banana fruit.
Not reportedComparator
Verbatim
e0“ethylene directly inhibited the degradation of phospholipids in banana and transgenic tomato fruits”
Claim · model-written summary
Ethylene directly inhibited phospholipid degradation in transgenic tomato fruit.
- Taxon
- tomato
Solanum lycopersicume0 - Treatment
- ethylenee0
- Outcome
- degradation of phospholipidse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene directly inhibited the degradation of phospholipids in banana and transgenic tomato fruits”
Claim · model-written summary
Ethylene reduced MaNAC25 and MaNAC28 activity, inhibiting phospholipid degradation.
Verbatim
e0“ethylene reduced the activity of MaNAC25 and MaNAC28, thereby inhibiting phospholipid degradation”
e1“cold tolerance in banana fruits”
The same questions in other papers
ethene · molecular: metabolites · MusaPositive and negative results
4 papers in the same direction as this paper · 1 reporting no difference from an alternative
- no effect
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.