Abstract
Abstract In vitro shoot proliferation and rooting of the apple scion cultivar ‘Macspur’ (mature culture) and of open-pollinated seedlings of ‘Macspur’ (seedling culture) were studied in response to the carbon sources, sorbitol, sucrose, glucose, and fructose, each supplied at 10, 30, 50, and 70 g/liter, or to the presence of varying proportions of sorbitol between 25% and 100% in conjunction with sucrose (30 g/liter total). Although the highest shoot number and shoot fresh weight of both cultures occurred with 30 g/liter sorbitol and sucrose, respectively, a medium with 25% sorbitol and 75% sucrose was best for shoot production. There was a decreasing trend in leaf/stem (fresh weight) ratio of shoots as the percentage of sorbitol increased in the medium. On rooting media supplied with 10–70 g/liter of each c source, rooting was best with 30 g/liter sucrose. In the mature culture (initially sucrose-grown), enhance shoot proliferation and rooting occurred on sorbitol medium after this source was subcultured 3 times on sorbitol medium.
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Claims 7
Claim · model-written summary
Shoot number was highest with 30 g/liter sorbitol in both apple cultures.
- Taxon
- apple
Malus domesticae0 - Material
- ‘Macspur’e0
- Treatment
- 30 g/liter sorbitole1
- Outcome
- shoot numbere1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“apple scion cultivar ‘Macspur’”
e1“the highest shoot number and shoot fresh weight of both cultures occurred with 30 g/liter sorbitol and sucrose, respectively”
Claim · model-written summary
Shoot fresh weight was highest with sucrose in both apple cultures.
- Taxon
- appleMalus domesticae0
- Material
- ‘Macspur’e0
- Treatment
- sucrosee1
- Outcome
- shoot fresh weighte1
- Direction
- higheste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“apple scion cultivar ‘Macspur’”
e1“the highest shoot number and shoot fresh weight of both cultures occurred with 30 g/liter sorbitol and sucrose, respectively”
Claim · model-written summary
A medium with 25% sorbitol and 75% sucrose was best for shoot production.
- Taxon
- appleMalus domesticae0
- Treatment
- 25% sorbitol and 75% sucrosee1
- Outcome
- shoot productione1
- Direction
- beste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“apple scion cultivar ‘Macspur’”
e1“a medium with 25% sorbitol and 75% sucrose was best for shoot production”
Claim · model-written summary
Leaf/stem fresh weight ratio decreased as sorbitol percentage increased.
- Taxon
- appleMalus domesticae0
- Treatment
- sorbitole1
- Outcome
- leaf/stem (fresh weight) ratio of shootse1
- Direction
- decreasinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“apple scion cultivar ‘Macspur’”
e1“There was a decreasing trend in leaf/stem (fresh weight) ratio of shoots as the percentage of sorbitol increased in the medium.”
Claim · model-written summary
Rooting was best with 30 g/liter sucrose.
- Taxon
- appleMalus domesticae0
- Treatment
- 30 g/liter sucrosee1
- Outcome
- rootinge1
- Direction
- beste1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“apple scion cultivar ‘Macspur’”
e1“rooting was best with 30 g/liter sucrose”
Claim · model-written summary
After three subcultures on sorbitol medium, mature apple cultures showed enhanced shoot proliferation.
- Taxon
- appleMalus domesticae0
- Material
- ‘Macspur’e0
- Treatment
- sorbitol mediume1
- Outcome
- shoot proliferatione1
- Direction
- enhancee1
- Qualifier
- subcultured 3 timese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“apple scion cultivar ‘Macspur’”
e1“In the mature culture (initially sucrose-grown), enhance shoot proliferation and rooting occurred on sorbitol medium after this source was subcultured 3 times on sorbitol medium.”
Claim · model-written summary
After three subcultures on sorbitol medium, mature apple cultures showed enhanced rooting.
- Taxon
- appleMalus domesticae0
- Material
- ‘Macspur’e0
- Treatment
- sorbitol mediume1
- Outcome
- rootinge1
- Direction
- enhancee1
- Qualifier
- subcultured 3 timese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“apple scion cultivar ‘Macspur’”
e1“In the mature culture (initially sucrose-grown), enhance shoot proliferation and rooting occurred on sorbitol medium after this source was subcultured 3 times on sorbitol medium.”
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