Paper · 2022 · Plants

Integrated Transcriptomic and Metabolomic Analysis of the Mechanism of Foliar Application of Hormone-Type Growth Regulator in the Improvement of Grape (Vitis vinifera L.) Coloration in Saline-Alkaline Soil

Doudou Chang; Huaijin Liu; Mengjie An; et al.

9
claims
19
verbatim quotes
14
citations
4
research areas
✓
Open access · cc-by

Abstract

(1) Background: To solve the problems of incomplete coloration and quality decline caused by unreasonable use of regulators in grapes, this study clarified the differences in the effects of a hormone-type growth regulator (AUT) and two commercial regulators on grape coloration and quality through field experiments. (2) Methods: The color indexes (brightness (L*), red/green color difference (a*), yellow/blue color difference (b*), and color index for red grapes (CIRG)) of grape fruit were measured using a CR-400 handheld color difference meter. The titratable acid content, total phenol content, and total sugar content were measured using anthrone colorimetry, folinol colorimetry, and NaOH titration, respectively, and the chalcone isomerase activity, phenylalanine ammoniase activity, dihydroflavol reductase activity, and anthocyanin content were measured using a UV spectrophotometer. (3) Results: The a*, total sugar and total phenol contents, and chalcone isomerase (CHI) and phenylalanine ammoniase (PAL) activities of grape fruit in the AUT treatment significantly increased, while the titratable acid content significantly decreased, compared to those in the CK treatment. The expressions of the differentially expressed genes (DEGs) trpB and argJ in AUT treatment were significantly up-regulated. The expressions of the differentially expressed metabolites (DEMs) phenylalanine and 4-oxoproline were significantly up-regulated, while those of 3,4-dihydroxybenzaldehyde and N-acetyl glutamate were significantly down-regulated. The CIRG significantly increased by 36.4% compared to that in the CK, indicating improved fruit coloration. (4) Conclusion: The AUT could shorten the color conversion period of grape fruit and improve the coloration, taste, and tolerance to saline and alkaline stresses.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 9

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The AUT treatment increased a*, total sugar and total phenol contents of grape fruit compared with the CK.

Taxon
grapee1
Organ / stage
grape fruite1
Treatment
AUT treatmente1
Outcome
a*, total sugar and total phenol contentse1
Direction
significantly increasede1
Comparator
CK treatmente1
Setting
field experimentse0

Verbatim

e0

“this study clarified the differences in the effects of a hormone-type growth regulator (AUT) and two commercial regulators on grape coloration and quality through field experiments”

✓ located verbatim in the source · abstract, characters 135–315 · show in text ↑
e1

“The a*, total sugar and total phenol contents, and chalcone isomerase (CHI) and phenylalanine ammoniase (PAL) activities of grape fruit in the AUT treatment significantly increased, while the titratable acid content significantly decreased, compared to those in the CK treatment”

✓ located verbatim in the source · abstract, characters 908–1186 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The AUT treatment increased chalcone isomerase and phenylalanine ammoniase activities in grape fruit compared with the CK.

Taxon
grapee1
Organ / stage
grape fruite1
Treatment
AUT treatmente1
Outcome
chalcone isomerase (CHI) and phenylalanine ammoniase (PAL) activitiese1
Direction
significantly increasede1
Comparator
CK treatmente1
Setting
field experimentse0

Verbatim

e0

“this study clarified the differences in the effects of a hormone-type growth regulator (AUT) and two commercial regulators on grape coloration and quality through field experiments”

✓ located verbatim in the source · abstract, characters 135–315 · show in text ↑
e1

“The a*, total sugar and total phenol contents, and chalcone isomerase (CHI) and phenylalanine ammoniase (PAL) activities of grape fruit in the AUT treatment significantly increased, while the titratable acid content significantly decreased, compared to those in the CK treatment”

✓ located verbatim in the source · abstract, characters 908–1186 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The AUT treatment decreased titratable acid content of grape fruit compared with the CK.

Taxon
grapee1
Organ / stage
grape fruite1
Treatment
AUT treatmente1
Outcome
titratable acid contente1
Direction
significantly decreasede1
Comparator
CK treatmente1
Setting
field experimentse0

Verbatim

e0

“this study clarified the differences in the effects of a hormone-type growth regulator (AUT) and two commercial regulators on grape coloration and quality through field experiments”

✓ located verbatim in the source · abstract, characters 135–315 · show in text ↑
e1

“The a*, total sugar and total phenol contents, and chalcone isomerase (CHI) and phenylalanine ammoniase (PAL) activities of grape fruit in the AUT treatment significantly increased, while the titratable acid content significantly decreased, compared to those in the CK treatment”

✓ located verbatim in the source · abstract, characters 908–1186 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

The AUT treatment significantly up-regulated expression of the DEGs trpB and argJ in grape fruit.

Taxon
grapee0
Treatment
AUT treatmente2
Gene
trpB and argJe2
Outcome
expressions of the differentially expressed genes (DEGs)e2
Direction
significantly up-regulatede2
Comparator
CK treatmente3
Setting
field experimentse0

Verbatim

e0

“this study clarified the differences in the effects of a hormone-type growth regulator (AUT) and two commercial regulators on grape coloration and quality through field experiments”

✓ located verbatim in the source · abstract, characters 135–315 · show in text ↑
e2

“The expressions of the differentially expressed genes (DEGs) trpB and argJ in AUT treatment were significantly up-regulated”

✓ located verbatim in the source · abstract, characters 1188–1311 · show in text ↑
e3

“compared to those in the CK treatment”

✓ located verbatim in the source · abstract, characters 1149–1186 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

The AUT treatment significantly up-regulated the metabolites phenylalanine and 4-oxoproline in grape fruit.

Taxon
grapee0
Treatment
AUTe0
Outcome
expressions of the differentially expressed metabolites (DEMs) phenylalanine and 4-oxoprolinee4
Direction
significantly up-regulatede4
Comparator
CK treatmente3
Setting
field experimentse0

Verbatim

e0

“this study clarified the differences in the effects of a hormone-type growth regulator (AUT) and two commercial regulators on grape coloration and quality through field experiments”

✓ located verbatim in the source · abstract, characters 135–315 · show in text ↑
e4

“The expressions of the differentially expressed metabolites (DEMs) phenylalanine and 4-oxoproline were significantly up-regulated”

✓ located verbatim in the source · abstract, characters 1313–1442 · show in text ↑
e3

“compared to those in the CK treatment”

✓ located verbatim in the source · abstract, characters 1149–1186 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

The AUT treatment significantly down-regulated 3,4-dihydroxybenzaldehyde and N-acetyl glutamate in grape fruit.

Taxon
grapee0
Treatment
AUTe0
Outcome
3,4-dihydroxybenzaldehyde and N-acetyl glutamatee5
Direction
significantly down-regulatede5
Comparator
CK treatmente3
Setting
field experimentse0

Verbatim

e0

“this study clarified the differences in the effects of a hormone-type growth regulator (AUT) and two commercial regulators on grape coloration and quality through field experiments”

✓ located verbatim in the source · abstract, characters 135–315 · show in text ↑
e5

“while those of 3,4-dihydroxybenzaldehyde and N-acetyl glutamate were significantly down-regulated”

✓ located verbatim in the source · abstract, characters 1444–1541 · show in text ↑
e3

“compared to those in the CK treatment”

✓ located verbatim in the source · abstract, characters 1149–1186 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The CIRG colour index of grape increased by 36.4% under AUT compared with the CK.

Taxon
grapee0
Treatment
AUTe0
Outcome
CIRGe6
Direction
significantly increasede6
Value
36.4%e6
Comparator
CKe6
Setting
field experimentse0

Verbatim

e0

“this study clarified the differences in the effects of a hormone-type growth regulator (AUT) and two commercial regulators on grape coloration and quality through field experiments”

✓ located verbatim in the source · abstract, characters 135–315 · show in text ↑
e6

“The CIRG significantly increased by 36.4% compared to that in the CK”

✓ located verbatim in the source · abstract, characters 1543–1611 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The AUT could shorten the colour conversion period of grape fruit.

Taxon
grapee7
Treatment
AUTe7
Outcome
color conversion periode7
Direction
shortene7

Not reportedComparator

Verbatim

e7

“The AUT could shorten the color conversion period of grape fruit and improve the coloration, taste, and tolerance to saline and alkaline stresses”

✓ located verbatim in the source · abstract, characters 1667–1812 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

The AUT could improve tolerance of grape to saline and alkaline stresses.

Taxon
grapee7
Treatment
AUTe7
Outcome
tolerance to saline and alkaline stressese7
Direction
improvee7

Not reportedComparator

Verbatim

e7

“The AUT could shorten the color conversion period of grape fruit and improve the coloration, taste, and tolerance to saline and alkaline stresses”

✓ located verbatim in the source · abstract, characters 1667–1812 · show in text ↑

class · quality: sugar starch · VitisPositive and negative results

5 papers: 3 positive, 2 negative or no effect

2 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

1 paper more in this group

See all 5 papers →

class · disease · VitisPositive and negative results

6 papers: 5 positive, 1 negative or no effect

4 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

class · quality: postharvest · VitisPositive and negative results

6 papers: 4 positive, 2 negative or no effect

3 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

2 papers more in this group

See all 6 papers →

class · molecular: metabolites · VitisPositive and negative results

9 papers: 8 positive, 1 negative or no effect

7 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.