Abstract
Plants often simultaneously experience combined stresses rather than a single stress, causing more serious damage, but the underlying mechanisms remain unknown. Here, we identified the stress-induced IbNAC3 from sweet potato (Ipomoea batatas) as a nucleus-localized transcription activator. IbNAC3 contains a unique activation domain whose MKD sequence confers transactivation activities to multiple other TFs and is essential for the activated expression of downstream target genes. Ectopic expression of IbNAC3 conferred tolerance to single and combined salt and drought stresses in Arabidopsis (Arabidopsis thaliana), and a group of NAM, ATAF1/2, and CUC2 (NAC) TFs, including ANAC011, ANAC072, ANAC083, ANAC100, and NAP, interacted with IbNAC3, and the specific domains responsible for each interaction varied. Intriguingly, IbNAC3 repressed the interaction among the five NACs, and knockout or mutation of ANAC011 and ANAC072 dramatically impaired combined stress tolerance. IbNAC3-ANAC072 and IbNAC3-NAP modules synergistically activated the MICROTUBULE-RELATED E3 LIGASE57 (MREL57) gene. Consistently, mutation of MREL57 and overexpression of WAVE-DAM-PENED2-LIKE7, encoding a target protein of MREL57, both remarkably impaired combined stress tolerance. Moreover, transgenic plants displayed abscisic acid (ABA) hyposensitivity by directly promoting the transcription of ENHANCED RESPONSE TO ABA 1, a key negative regulator of ABA signaling. The data unravel the unique IbNAC3 TF functions as a pivotal component in combined stress tolerance by integrating multiple regulatory events and ubiquitin pathways, which is essential for developing high-tolerant plants in natural environments.
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Claims 13
Claim · model-written summary
IbNAC3 from sweet potato is a stress-induced, nucleus-localized transcription activator.
- Taxon
- sweet potato
_Flower.jpg?width=60)
Ipomoea batatase1 - Gene
- IbNAC3e1
- Outcome
- nucleus-localized transcription activatore1
- Qualifier
- stress-inducede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Flower.jpg?width=500)
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“we identified the stress-induced IbNAC3 from sweet potato (Ipomoea batatas) as a nucleus-localized transcription activator”
Claim · model-written summary
The MKD sequence in the IbNAC3 activation domain confers transactivation activities to multiple other transcription factors.
- Taxon
- sweet potatoIpomoea batatast0
- Gene
- IbNAC3e1
- Outcome
- transactivation activitiese1
- Qualifier
- MKD sequencee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“IbNAC3 contains a unique activation domain whose MKD sequence confers transactivation activities to multiple other TFs and is essential for the activated expression of downstream target genes”
Claim · model-written summary
Ectopic expression of IbNAC3 increased tolerance to single and combined salt and drought stresses in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- Ectopic expression of IbNAC3e1
- Gene
- IbNAC3e1
- Outcome
- tolerance to single and combined salt and drought stressese1
- Direction
- increaseinferrede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“Ectopic expression of IbNAC3 conferred tolerance to single and combined salt and drought stresses in Arabidopsis”
Claim · model-written summary
ANAC011, ANAC072 and ANAC083 interacted with IbNAC3.
- Taxon
- sweet potatoIpomoea batatast0
- Gene
- ANAC011, ANAC072, ANAC083e1
- Outcome
- interacted with IbNAC3e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“a group of NAM, ATAF1/2, and CUC2 (NAC) TFs, including ANAC011, ANAC072, ANAC083, ANAC100, and NAP, interacted with IbNAC3”
Claim · model-written summary
ANAC100 and NAP interacted with IbNAC3.
- Taxon
- sweet potatoIpomoea batatast0
- Gene
- ANAC100, and NAPe1
- Outcome
- interacted with IbNAC3e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“a group of NAM, ATAF1/2, and CUC2 (NAC) TFs, including ANAC011, ANAC072, ANAC083, ANAC100, and NAP, interacted with IbNAC3”
Claim · model-written summary
The specific domains responsible for the interactions of IbNAC3 with each NAC transcription factor varied.
- Taxon
- sweet potatoIpomoea batatast0
- Outcome
- specific domains responsible for each interactione1
- Qualifier
- variede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“the specific domains responsible for each interaction varied”
Claim · model-written summary
IbNAC3 repressed the interaction among the five NAC transcription factors.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- IbNAC3e1
- Outcome
- interaction among the five NACse1
- Direction
- repressede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“IbNAC3 repressed the interaction among the five NACs”
Claim · model-written summary
Knockout or mutation of ANAC011 and ANAC072 impaired combined stress tolerance.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- knockout or mutation of ANAC011 and ANAC072e1
- Gene
- ANAC011 and ANAC072e1
- Outcome
- combined stress tolerancee1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“knockout or mutation of ANAC011 and ANAC072 dramatically impaired combined stress tolerance”
Claim · model-written summary
IbNAC3-ANAC072 and IbNAC3-NAP modules synergistically activated the MREL57 gene.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- IbNAC3-ANAC072 and IbNAC3-NAP modulese1
- Gene
- MREL57e1
- Outcome
- MICROTUBULE-RELATED E3 LIGASE57 (MREL57) genee1
- Direction
- activatede1
- Qualifier
- synergisticallye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“IbNAC3-ANAC072 and IbNAC3-NAP modules synergistically activated the MICROTUBULE-RELATED E3 LIGASE57 (MREL57) gene”
Claim · model-written summary
Mutation of MREL57 and overexpression of WAVE-DAM-PENED2-LIKE7 both impaired combined stress tolerance.
- Taxon
- sweet potatoIpomoea batatast0
- Treatment
- mutation of MREL57 and overexpression of WAVE-DAM-PENED2-LIKE7e1
- Gene
- MREL57e1
- Outcome
- combined stress tolerancee1
- Direction
- impairede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“mutation of MREL57 and overexpression of WAVE-DAM-PENED2-LIKE7, encoding a target protein of MREL57, both remarkably impaired combined stress tolerance”
Claim · model-written summary
Transgenic plants displayed abscisic acid hyposensitivity.
- Taxon
- sweet potatoIpomoea batatast0
- Material
- transgenic plantse1
- Outcome
- hyposensitivitye1
- Qualifier
- abscisic acid (ABA)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“transgenic plants displayed abscisic acid (ABA) hyposensitivity by directly promoting the transcription of ENHANCED RESPONSE TO ABA 1”
Claim · model-written summary
Transgenic plants directly promote transcription of ENHANCED RESPONSE TO ABA 1.
- Taxon
- sweet potatoIpomoea batatast0
- Gene
- ENHANCED RESPONSE TO ABA 1e1
- Outcome
- transcriptione1
- Qualifier
- directly promotinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“transgenic plants displayed abscisic acid (ABA) hyposensitivity by directly promoting the transcription of ENHANCED RESPONSE TO ABA 1, a key negative regulator of ABA signaling”
Claim · model-written summary
IbNAC3 is a pivotal component of combined stress tolerance by integrating multiple regulatory events and ubiquitin pathways.
- Taxon
- sweet potatoIpomoea batatast0
- Gene
- IbNAC3e1
- Outcome
- combined stress tolerancee1
- Qualifier
- by integrating multiple regulatory events and ubiquitin pathwayse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“sweet potato NAC transcription factor IbNAC3”
e1“The data unravel the unique IbNAC3 TF functions as a pivotal component in combined stress tolerance by integrating multiple regulatory events and ubiquitin pathways”
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