Abstract
Abstract The plant growth-boosting biofilm-forming bacteria Bacillus pseudomycoides is able to promote growth and drought stress tolerance in wheat by suppressing the MYB gene, which synthesizes Myb protein (TaMpc1-D4) through secreted volatile compounds. In the present study, Triticum aestivum seeds were inoculated with five distinct bacterial strains. The growth, germination rate, root-shoot length, RWC, and chlorophyll content of seedlings were investigated. Furthermore, the levels of soluble sugars, proteins, H 2 O 2 , NO, cell death, and antioxidant enzymes (CAT, SOD, POD, and APX) were observed throughout the growth stage. All of the results showed that B. pseudomycoides had a substantially higher ability to form biofilm and promote these traits than the other strains. In terms of molecular gene expression, B. pseudomycoides inoculation strongly expressed the Dreb1 gene by silencing the expression of MYB gene through secreted volatile compounds. For identifying the specific volatile compound that silenced the MYB gene, molecular docking with Myb protein was performed. Out of 45 volatile compounds found, 2,6-ditert-butylcyclohexa-2,5-diene-1,4-dione and 3,5-ditert-butylphenol had a binding free energy of − 6.2 and − 6.5, Kcal/mol, respectively, which predicted that these compounds could suppress this protein's expression. In molecular dynamics simulations, the RMSD, SASA, Rg, RMSF, and hydrogen bonding values found assured the docked complexes' binding stability. These findings suggest that these targeted compounds may be suppressing Myb protein expression as well as the expression of Dreb1 and other drought response genes in wheat. More research (field trial) into plant growth and drought stress is needed to support the findings of this study.
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Claims 8
Claim · model-written summary
B. pseudomycoides inoculation gave a substantially higher ability to form biofilm and promote growth-related traits than the other strains.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- inoculated with five distinct bacterial strainse2
- Interactor
- B. pseudomycoidesBacillus pseudomycoidese1symbiont
- Outcome
- ability to form biofilm and promote these traitse1
- Direction
- highere1
- Comparator
- the other strainse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e1“All of the results showed that B. pseudomycoides had a substantially higher ability to form biofilm and promote these traits than the other strains”
e2“In the present study, Triticum aestivum seeds were inoculated with five distinct bacterial strains”
Claim · model-written summary
B. pseudomycoides inoculation strongly increased Dreb1 gene expression in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- B. pseudomycoides inoculatione3
- Gene
- Dreb1 genee3
- Outcome
- gene expressione3
- Direction
- increaseinferrede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e3“In terms of molecular gene expression, B. pseudomycoides inoculation strongly expressed the Dreb1 gene”
Claim · model-written summary
Volatile compounds from B. pseudomycoides decreased MYB gene expression in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- secreted volatile compoundse4
- Gene
- MYB genee4
- Outcome
- expression of MYB genee4
- Direction
- decreaseinferrede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e4“by silencing the expression of MYB gene through secreted volatile compounds”
Claim · model-written summary
Docking predicted a binding free energy of −6.2 Kcal/mol for 2,6-ditert-butylcyclohexa-2,5-diene-1,4-dione with Myb protein.
- Taxon
- wheatTriticum aestivumt0
- Compound
- 2,6-ditert-butylcyclohexa-2,5-diene-1,4-dionee5
- Outcome
- binding free energye5
- Value
- − 6.2e5
- Qualifier
- Kcal/mole5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e5“2,6-ditert-butylcyclohexa-2,5-diene-1,4-dione and 3,5-ditert-butylphenol had a binding free energy of − 6.2 and − 6.5, Kcal/mol, respectively”
Claim · model-written summary
Docking predicted a binding free energy of −6.5 Kcal/mol for 3,5-ditert-butylphenol with Myb protein.
- Taxon
- wheatTriticum aestivumt0
- Compound
- 3,5-ditert-butylphenole5
- Outcome
- binding free energye5
- Value
- − 6.5e5
- Qualifier
- Kcal/mole5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e5“2,6-ditert-butylcyclohexa-2,5-diene-1,4-dione and 3,5-ditert-butylphenol had a binding free energy of − 6.2 and − 6.5, Kcal/mol, respectively”
Claim · model-written summary
Forty-five volatile compounds were found.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- volatile compounds founde6
- Value
- 45 volatile compoundse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e6“Out of 45 volatile compounds found”
Claim · model-written summary
Molecular dynamics showed that the docked complexes were binding-stable.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- binding stabilitye7
- Qualifier
- RMSD, SASA, Rg, RMSF, and hydrogen bonding valuese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e7“In molecular dynamics simulations, the RMSD, SASA, Rg, RMSF, and hydrogen bonding values found assured the docked complexes' binding stability”
Claim · model-written summary
The targeted compounds may suppress Myb protein expression and the expression of Dreb1 and other drought response genes in wheat.
- Taxon
- wheatTriticum aestivumt0
- Compound
- these targeted compoundse0
- Outcome
- Myb protein expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat (Triticum aestivum L.)”
e0“These findings suggest that these targeted compounds may be suppressing Myb protein expression as well as the expression of Dreb1 and other drought response genes in wheat”
The same questions in other papers
inoculation · molecular: expression · Triticum aestivum (wheat)Positive and negative results
37 papers in the same direction as this paper · 9 in the opposite direction
- no effect
- no effect
- no effect
43 papers more in this group
See all 47 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.