Abstract
Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development. Sucrose non-fermenting 1-related protein kinase 2 (SnRK2) acts as a signaling hub in the response to drought stress, but whether SnRK2 helps plants cope with water deficit via other mechanisms is largely unknown. Here, we cloned and characterized TaSnRK2.10, which was induced by multiple abiotic stresses and phytohormones. Ectopic expression of TaSnRK2.10 in rice (Oryza sativa) conferred drought tolerance, manifested by multiple improved physiological indices, including increased water content, cell membrane stability, and survival rates, as well as decreased water loss and accumulation of H2O2 and malonaldehyde. TaSnRK2.10 interacted with and phosphorylated early responsive to dehydration 15 (TaERD15) and enolase 1 (TaENO1) in vivo and in vitro. TaERD15 phosphorylated by TaSnRK2.10 was prone to degradation by the 26S proteasome, thereby mitigating its negative effects on drought tolerance. Phosphorylation of TaENO1 by TaSnRK2.10 may account for the substantially increased levels of phosphoenolpyruvate (PEP), a key metabolite of primary and secondary metabolism, in TaSnRK2.10-overexpressing rice, thereby enhancing its viability under drought stress. Our results demonstrate that TaSnRK2.10 not only regulated stomatal aperture and the expression of drought-responsive genes, but also enhanced PEP supply and promoted the degradation of TaERD15, all of which enhanced drought tolerance.
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Claims 10
Claim · model-written summary
Ectopic expression of TaSnRK2.10 conferred drought tolerance in rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- Ectopic expression of TaSnRK2.10e0
- Gene
- TaSnRK2.10e0
- Outcome
- drought tolerancee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of TaSnRK2.10 in rice (Oryza sativa) conferred drought tolerance, manifested by multiple improved physiological indices, including increased water content, cell membrane stability, and survival rates, as well as decreased water loss and accumulation of H2O2 and malonaldehyde”
Claim · model-written summary
Ectopic expression of TaSnRK2.10 increased water content, cell membrane stability and survival rates in rice under drought.
- Taxon
- riceOryza sativae0
- Treatment
- Ectopic expression of TaSnRK2.10e0
- Gene
- TaSnRK2.10e0
- Outcome
- water content, cell membrane stability, and survival ratese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of TaSnRK2.10 in rice (Oryza sativa) conferred drought tolerance, manifested by multiple improved physiological indices, including increased water content, cell membrane stability, and survival rates, as well as decreased water loss and accumulation of H2O2 and malonaldehyde”
Claim · model-written summary
Ectopic expression of TaSnRK2.10 decreased water loss and accumulation of H2O2 and malonaldehyde in rice.
- Taxon
- riceOryza sativae0
- Treatment
- Ectopic expression of TaSnRK2.10e0
- Gene
- TaSnRK2.10e0
- Outcome
- water loss and accumulation of H2O2 and malonaldehydee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ectopic expression of TaSnRK2.10 in rice (Oryza sativa) conferred drought tolerance, manifested by multiple improved physiological indices, including increased water content, cell membrane stability, and survival rates, as well as decreased water loss and accumulation of H2O2 and malonaldehyde”
Claim · model-written summary
TaSnRK2.10 interacts with and phosphorylates TaERD15 in vivo and in vitro.
- Taxon
- Wheat
Triticum aestivume2 - Gene
- TaERD15e1
- Outcome
- interacted with and phosphorylatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development”
e1“TaSnRK2.10 interacted with and phosphorylated early responsive to dehydration 15 (TaERD15) and enolase 1 (TaENO1) in vivo and in vitro”
Claim · model-written summary
TaSnRK2.10 interacts with and phosphorylates TaENO1 in vivo and in vitro.
- Taxon
- WheatTriticum aestivume2
- Gene
- TaENO1e1
- Outcome
- interacted with and phosphorylatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development”
e1“TaSnRK2.10 interacted with and phosphorylated early responsive to dehydration 15 (TaERD15) and enolase 1 (TaENO1) in vivo and in vitro”
Claim · model-written summary
TaERD15 phosphorylated by TaSnRK2.10 is prone to degradation by the 26S proteasome, mitigating its negative effect on drought tolerance.
- Taxon
- WheatTriticum aestivume2
- Gene
- TaERD15e6
- Outcome
- prone to degradatione6
- Qualifier
- thereby mitigating its negative effects on drought tolerancee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development”
e6“TaERD15 phosphorylated by TaSnRK2.10 was prone to degradation by the 26S proteasome, thereby mitigating its negative effects on drought tolerance”
Claim · model-written summary
TaSnRK2.10-overexpressing rice shows substantially increased levels of phosphoenolpyruvate (PEP).
- Taxon
- riceOryza sativae3
- Treatment
- TaSnRK2.10-overexpressing ricee3
- Outcome
- levels of phosphoenolpyruvate (PEP)e3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Phosphorylation of TaENO1 by TaSnRK2.10 may account for the substantially increased levels of phosphoenolpyruvate (PEP), a key metabolite of primary and secondary metabolism, in TaSnRK2.10-overexpressing rice”
Claim · model-written summary
Phosphorylation of TaENO1 by TaSnRK2.10 may account for the increased PEP levels in rice.
- Taxon
- riceOryza sativae3
- Gene
- TaENO1e3
- Outcome
- levels of phosphoenolpyruvate (PEP)e3
- Qualifier
- may account fore3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Phosphorylation of TaENO1 by TaSnRK2.10 may account for the substantially increased levels of phosphoenolpyruvate (PEP), a key metabolite of primary and secondary metabolism, in TaSnRK2.10-overexpressing rice”
Claim · model-written summary
TaSnRK2.10 regulates stomatal aperture and the expression of drought-responsive genes.
- Taxon
- WheatTriticum aestivume2
- Gene
- TaSnRK2.10e4
- Outcome
- stomatal aperture and the expression of drought-responsive genese4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development”
e4“TaSnRK2.10 not only regulated stomatal aperture and the expression of drought-responsive genes”
Claim · model-written summary
TaSnRK2.10 expression is induced by multiple abiotic stresses and phytohormones in wheat.
- Taxon
- WheatTriticum aestivume2
- Gene
- TaSnRK2.10e5
- Outcome
- induced by multiple abiotic stresses and phytohormonese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Wheat (Triticum aestivum) is particularly susceptible to water deficit at the jointing stage of its development”
e5“Here, we cloned and characterized TaSnRK2.10, which was induced by multiple abiotic stresses and phytohormones”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.