Abstract
Browning limits the commercial value of fresh-cut lotus root slices. Melatonin has been reported to play crucial plant roles in growth and development. However, the mechanisms in repressing the browning of fresh-cut lotuses are still unclear. In this study, fresh-cut lotus root slices were treated with melatonin, the physical signs of browning were tested, and then the selected samples (0 d, 6 d, 12 d) were used in multiomics analysis. Fresh-cut lotus root slices with a thickness of 4 mm were soaked in a 40 mmol/L melatonin solution for 10 min; then, the slices were packed in pallets and packages and stored at 10 ± 1 °C. The results show that the 40 mmol/L melatonin selected for repressing the browning of lotus roots significantly delayed the decrease in water, total soluble solid content, and Vitamin C, decreased the growth of microorganisms, enhanced total phenolic content, improved total antioxidant capacity, and decreased ·OH, H2O2, and O2−· contents. Moreover, this treatment enhanced phenylalanine ammonialyase, polyphenol oxidase, superoxide dismutase, and catalase activities and reduced peroxidase activities and soluble quinones. NnSOD (104590242), NnCAT (104609297), and some NnPOD genes showed a similar transcript accumulation pattern with enzyme activity. It can be seen from these results that exogenous melatonin accelerated an enhancement in the antioxidant system and AsA-GSH cycle system by regulating ROS-metabolism-related genes, thereby improving the capacity to withstand browning and the quality of lotus root slices. The microbiome also showed that melatonin suppressed the fertility of spoilage organisms, such as Pseudomonas, Tolumonas, Acinetobacter, Stenotrophomonas, and Proteobacteria. Metabonomics data uncovered that the metabolites of flavonoid biosynthesis, phenylpropanoid biosynthesis, tyrosine metabolism, and phenylalanine metabolism were involved in the process.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Melatonin delayed loss of water, soluble solids and Vitamin C in fresh-cut lotus roots.
- Treatment
- 40 mmol/L melatonine0
- Outcome
- water, total soluble solid content, and Vitamin Ce0
- Direction
- delayed the decreasee0
.jpg?width=60){kind=small}
LotusNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e0“40 mmol/L melatonin selected for repressing the browning of lotus roots significantly delayed the decrease in water, total soluble solid content, and Vitamin C”
Claim · model-written summary
Melatonin decreased microorganism growth on lotus roots.
- Treatment
- melatonine1
- Interactor
- microorganismse0other
- Outcome
- growth of microorganismse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e1“40 mmol/L melatonin selected for repressing the browning of lotus roots significantly delayed the decrease in water, total soluble solid content, and Vitamin C”
e0“decreased the growth of microorganisms”
Claim · model-written summary
Melatonin enhanced total phenolic content.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e1“40 mmol/L melatonin selected for repressing the browning of lotus roots significantly delayed the decrease in water, total soluble solid content, and Vitamin C”
e0“enhanced total phenolic content”
Claim · model-written summary
Melatonin improved total antioxidant capacity.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e1“40 mmol/L melatonin selected for repressing the browning of lotus roots significantly delayed the decrease in water, total soluble solid content, and Vitamin C”
e0“improved total antioxidant capacity”
Claim · model-written summary
Melatonin lowered reactive oxygen species contents.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e1“40 mmol/L melatonin selected for repressing the browning of lotus roots significantly delayed the decrease in water, total soluble solid content, and Vitamin C”
e0“decreased ·OH, H2O2, and O2−· contents”
Claim · model-written summary
Melatonin enhanced phenylalanine ammonialyase, polyphenol oxidase and superoxide dismutase activities.
- Treatment
- this treatmente0
- Outcome
- phenylalanine ammonialyase, polyphenol oxidase, superoxide dismutase, and catalase activitiese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e0“this treatment enhanced phenylalanine ammonialyase, polyphenol oxidase, superoxide dismutase, and catalase activities”
Claim · model-written summary
Melatonin reduced peroxidase activities and soluble quinones.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e1“40 mmol/L melatonin selected for repressing the browning of lotus roots significantly delayed the decrease in water, total soluble solid content, and Vitamin C”
e0“reduced peroxidase activities and soluble quinones”
Claim · model-written summary
NnSOD, NnCAT and some NnPOD transcripts followed a pattern similar to enzyme activity.
- Gene
- NnSODe0
- Outcome
- transcript accumulation patterne0
- Qualifier
- similar transcript accumulation pattern with enzyme activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e0“NnSOD (104590242), NnCAT (104609297), and some NnPOD genes showed a similar transcript accumulation pattern with enzyme activity.”
Claim · model-written summary
The authors infer that melatonin enhanced antioxidant and AsA-GSH systems through ROS-related genes, improving browning resistance and quality.
- Treatment
- exogenous melatonine0
- Gene
- ROS-metabolism-related genese0
- Outcome
- capacity to withstand browning and the quality of lotus root slicese0
- Qualifier
- antioxidant system and AsA-GSH cycle systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e0“exogenous melatonin accelerated an enhancement in the antioxidant system and AsA-GSH cycle system by regulating ROS-metabolism-related genes, thereby improving the capacity to withstand browning and the quality of lotus root slices.”
Claim · model-written summary
Melatonin suppressed spoilage organisms in the lotus microbiome.
- Treatment
- melatonine0
- Interactor
- Pseudomonas
Pseudomonase0other - Outcome
- fertility of spoilage organismse0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e0“melatonin suppressed the fertility of spoilage organisms, such as Pseudomonas, Tolumonas, Acinetobacter, Stenotrophomonas, and Proteobacteria.”
Claim · model-written summary
Metabolites from four metabolic pathways were implicated in the browning process.
- Outcome
- metabolites of flavonoid biosynthesis, phenylpropanoid biosynthesis, tyrosine metabolism, and phenylalanine metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“lotus (Nelumbo nucifera Gaertn.)”
e0“metabolites of flavonoid biosynthesis, phenylpropanoid biosynthesis, tyrosine metabolism, and phenylalanine metabolism were involved in the process.”