Abstract
Analyzing the molecular and physiological processes that govern the uptake and transport of nitrogen (N) in plants is central to efforts to fully understand the optimization of plant N use and the changes in the N-use efficiency in relation to changes in atmospheric N deposition changes. Here, a field experiment was conducted using the ectomycorrhizal fungi (EMF), Pisolithus tinctorius (Pt) and Suillus grevillei (Sg). The effects of N deposition were investigated using concentrations of 0 kg·N·hm−2a−1 (N0), a normal N deposition of 30 kg·N·hm−2a−1 (N30), a moderate N deposition of 60 kg·N·hm−2a−1 (N60), and a severe N deposition of 90 kg·N·hm−2a−1 (N90), with the goal of examining how these factors impacted root activity, root absorbing area, NH4+ and NO3− uptake kinetics, and the expression of ammonium and nitrate transporter genes in Pinus massoniana seedlings under different levels of N deposition. These data revealed that EMF inoculation led to increased root dry weight, activity, and absorbing area. The NH4+ and NO3− uptake kinetics in seedlings conformed to the Michaelis–Menten equation, and uptake rates declined with increasing levels of N addition, with NH4+ uptake rates remaining higher than NO3− uptake rates for all tested concentrations. EMF inoculation was associated with higher Vmax values than were observed for non-mycorrhizal plants. Nitrogen addition resulted in the upregulation of genes in the AMT1 family and the downregulation of genes in the NRT family. EMF inoculation under the N60 and N90 treatment conditions resulted in the increased expression of each of both these gene families. NH4+ and NO3− uptake kinetics were also positively correlated with associated transporter gene expression in P. massoniana roots. Together, these data offer a theoretical foundation for EMF inoculation under conditions of increased N deposition associated with climate change in an effort to improve N absorption and transport rates through the regulation of key nitrogen transporter genes, thereby enhancing N utilization efficiency and promoting plant growth. Synopsis: EMF could enhance the efficiency of N utilization and promote the growth of Pinus massoniana under conditions of increased N deposition.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
EMF inoculation increased root dry weight, activity, and absorbing area.
- Taxon
- Pinus massoniana
Pinus massonianat0 - Treatment
- EMF inoculatione0
- Interactor
- ectomycorrhizal fungit0symbiont
- Outcome
- root dry weight, activity, and absorbing areae0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EMF inoculation led to increased root dry weight, activity, and absorbing area.”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
Claim · model-written summary
Seedling ammonium and nitrate uptake kinetics conformed to the Michaelis–Menten equation.
Verbatim
e0“The NH4+ and NO3− uptake kinetics in seedlings conformed to the Michaelis–Menten equation”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
Claim · model-written summary
Nitrogen uptake rates declined as nitrogen addition increased.
- Taxon
- Pinus massonianaPinus massonianat0
- Treatment
- N additione0
- Outcome
- uptake ratese0
- Direction
- declinede0
- Comparator
- increasing levels of N additione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“uptake rates declined with increasing levels of N addition”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
Claim · model-written summary
Ammonium uptake rates exceeded nitrate uptake rates at all tested concentrations.
- Taxon
- Pinus massonianaPinus massonianat0
- Outcome
- NH4+ uptake ratese0
- Direction
- highere0
- Qualifier
- for all tested concentrationse0
- Comparator
- NO3− uptake ratese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NH4+ uptake rates remaining higher than NO3− uptake rates for all tested concentrations”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
Claim · model-written summary
EMF inoculation was associated with higher Vmax than in non-mycorrhizal plants.
Verbatim
e0“EMF inoculation was associated with higher Vmax values than were observed for non-mycorrhizal plants.”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
Claim · model-written summary
Nitrogen addition upregulated AMT1 family genes.
- Taxon
- Pinus massonianaPinus massonianat0
- Treatment
- Nitrogen additione0
- Gene
- AMT1e0
- Outcome
- genes in the AMT1 familye0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrogen addition resulted in the upregulation of genes in the AMT1 family”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
Claim · model-written summary
Nitrogen addition downregulated NRT family genes.
- Taxon
- Pinus massonianaPinus massonianat0
- Treatment
- Nitrogen additione0
- Gene
- NRTe0
- Outcome
- genes in the NRT familye0
- Direction
- downregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrogen addition resulted in the upregulation of genes in the AMT1 family and the downregulation of genes in the NRT family”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
Claim · model-written summary
EMF inoculation increased expression of both transporter gene families under N60 and N90.
- Taxon
- Pinus massonianaPinus massonianat0
- Treatment
- EMF inoculatione0
- Interactor
- ectomycorrhizal fungit0symbiont
- Outcome
- expression of each of both these gene familiese0
- Direction
- increasede0
- Qualifier
- under the N60 and N90 treatment conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EMF inoculation under the N60 and N90 treatment conditions resulted in the increased expression of each of both these gene families.”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
Claim · model-written summary
Ammonium and nitrate uptake kinetics were positively correlated with associated transporter gene expression in roots.
- Taxon
- P. massonianaPinus massonianae0
- Organ / stage
- rootse0
- Gene
- transporter genee0
- Outcome
- NH4+ and NO3− uptake kineticse0
- Direction
- positively correlatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NH4+ and NO3− uptake kinetics were also positively correlated with associated transporter gene expression in P. massoniana roots.”
t0“The Relationship between Ectomycorrhizal Fungi, Nitrogen Deposition, and Pinus massoniana Seedling Nitrogen Transporter Gene Expression and Nitrogen Uptake Kinetics”
The same questions in other papers
inoculation · growth · Pinus massoniana (Chinese red pine)Positive and negative results
11 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
9 papers more in this group
See all 13 papers →inoculation · molecular: expression · Pinus massoniana (Chinese red pine)Results agree
2 papers in the same direction as this paper
- positive
- positive
mycorrhiza · growth · Pinus massoniana (Chinese red pine)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.