Abstract
Abstract Raphanus sativus (radish) tuber extracts stock solutions were prepared by dissolving radish extracts prepared with certain solvents (distilled water, ethanol and methanol) in dimethyl sulfoxide. Negative control (dimethyl sulfoxide, DMSO), positive control mutagen (ethyl methanesulfonate) and R. sativus extracts (10 and 20 mg/mL doses) were applied to Allium cepa root tips at for 24 and 48 h. For examination under the light microscope, the root tips were placed in fixative, then hydrolyzed and treated with acetocarmine. Chromosomal aberration index (CAI%) was increased as compared with the negative control, but decreased as compared with the positive control after the application of three different extracts. It was put forward that mitotix index decreased with increasing extract concentration applications. In this way, it has been determined that these extracts have an inhibitory effect on cell division at high doses. It has been found that CAI% was an increase with increasing extract concentration. It has been revealed that distilled water, ethanol and methanol extracts have more inhibitory effect on cell division and an increasing frequency of chromosomal anomalies, respectively. As a result of this research, R. sativus tuber might cause damage to cells genetic material and spindle formation when used incorrectly.
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Claims 7
Claim · model-written summary
Radish tuber extracts increased the chromosomal aberration index in Allium cepa root tips compared with the negative control.
- Taxon
- Allium cepa
Allium cepae1 - Treatment
- R. sativus extractse1
- Outcome
- Chromosomal aberration index (CAI%)e2
- Direction
- increasede2
- Comparator
- negative controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Negative control (dimethyl sulfoxide, DMSO), positive control mutagen (ethyl methanesulfonate) and R. sativus extracts (10 and 20 mg/mL doses) were applied to Allium cepa root tips at for 24 and 48 h”
e2“Chromosomal aberration index (CAI%) was increased as compared with the negative control, but decreased as compared with the positive control after the application of three different extracts”
Claim · model-written summary
Radish tuber extracts decreased the chromosomal aberration index in Allium cepa root tips compared with the positive control.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- R. sativus extractse1
- Outcome
- Chromosomal aberration index (CAI%)e2
- Direction
- decreasede2
- Comparator
- positive controle2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Negative control (dimethyl sulfoxide, DMSO), positive control mutagen (ethyl methanesulfonate) and R. sativus extracts (10 and 20 mg/mL doses) were applied to Allium cepa root tips at for 24 and 48 h”
e2“Chromosomal aberration index (CAI%) was increased as compared with the negative control, but decreased as compared with the positive control after the application of three different extracts”
Claim · model-written summary
The mitotic index of Allium cepa root tips decreased with increasing radish extract concentration.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- increasing extract concentratione2
- Outcome
- mitotix indexe2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Negative control (dimethyl sulfoxide, DMSO), positive control mutagen (ethyl methanesulfonate) and R. sativus extracts (10 and 20 mg/mL doses) were applied to Allium cepa root tips at for 24 and 48 h”
e2“It was put forward that mitotix index decreased with increasing extract concentration applications”
Claim · model-written summary
The chromosomal aberration index of Allium cepa increased with increasing radish extract concentration.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- increasing extract concentratione2
- Outcome
- CAI%e2
- Direction
- an increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Negative control (dimethyl sulfoxide, DMSO), positive control mutagen (ethyl methanesulfonate) and R. sativus extracts (10 and 20 mg/mL doses) were applied to Allium cepa root tips at for 24 and 48 h”
e2“It has been found that CAI% was an increase with increasing extract concentration”
Claim · model-written summary
Distilled water, ethanol and methanol extracts had an inhibitory effect on cell division, distilled water extracts the most.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- distilled water, ethanol and methanol extractse2
- Outcome
- inhibitory effect on cell divisione2
- Direction
- moree2
- Qualifier
- respectivelye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Negative control (dimethyl sulfoxide, DMSO), positive control mutagen (ethyl methanesulfonate) and R. sativus extracts (10 and 20 mg/mL doses) were applied to Allium cepa root tips at for 24 and 48 h”
e2“It has been revealed that distilled water, ethanol and methanol extracts have more inhibitory effect on cell division and an increasing frequency of chromosomal anomalies, respectively”
Claim · model-written summary
Distilled water, ethanol and methanol extracts increased the frequency of chromosomal anomalies, distilled water extracts the least.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- distilled water, ethanol and methanol extractse2
- Outcome
- frequency of chromosomal anomaliese2
- Direction
- increasinge2
- Qualifier
- respectivelye2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Negative control (dimethyl sulfoxide, DMSO), positive control mutagen (ethyl methanesulfonate) and R. sativus extracts (10 and 20 mg/mL doses) were applied to Allium cepa root tips at for 24 and 48 h”
e2“It has been revealed that distilled water, ethanol and methanol extracts have more inhibitory effect on cell division and an increasing frequency of chromosomal anomalies, respectively”
Claim · model-written summary
Radish tuber might damage genetic material and spindle formation in cells when used incorrectly.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- R. sativus tubere2
- Outcome
- damage to cells genetic material and spindle formatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Negative control (dimethyl sulfoxide, DMSO), positive control mutagen (ethyl methanesulfonate) and R. sativus extracts (10 and 20 mg/mL doses) were applied to Allium cepa root tips at for 24 and 48 h”
e2“R. sativus tuber might cause damage to cells genetic material and spindle formation when used incorrectly”
The same questions in other papers
botanical · cytology · Allium cepa (onion)Positive and negative results
25 papers in the same direction as this paper · 89 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
112 papers more in this group
See all 116 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.