Abstract
This study aimed to isolate, screen the plant-growth-enhancing features, and explore the whole-genome sequence of AZC66 isolated from the rhizosphere of Zygophyllum coccineum and determine its biostimulating effects on the growth of cowpea under greenhouse conditions. Salkowski reagent was used to measure AZC66’s indole acetic acid production. AZC66’s inorganic phosphate solubility on Pikovskaya agar was evaluated using tricalcium phosphate. The results indicated the ability of AZC66 to fix nitrogen, produce IAA (66.33 ± 0.44 μg mL−1), solubilize inorganic phosphate, and exhibit the activity of ACC deaminase (278.40 ± 21 mol -ketobutyrate mg−1 h−1). Cowpea’s root and shoot dry weights were also significantly increased after in vitro inoculation with AZC66. The identity of AZC66 was confirmed as Priestia filamentosa, and 4840 genes were predicted in its genome. The gene sequences were compared against the Kyoto Encyclopedia of Genes and Genomes (KEGG) database, and the results showed that the top three pathways wherein the maximum number of genes are involved are signaling and cellular processes, genetic information processing, and carbohydrate metabolism. The genome sequencing of the strain AZC66 revealed a number of genes implicated in plant biostimulation activities such as nitrogen fixation (nifU), phytohormone synthesis (trpAB genes), phosphate solubilization (PhbCEF, pstABCS, and phoU), and siderophore formation (FbpA, feoAB, and fetB). The AZC66 genome contained numerous genes involved in nitrogen metabolism, nitrogen regulation, and the nitrate reduction pathway. The phenazine biosynthetic gene in AZC66 demonstrated biocontrol and soil survival properties. The trehalose synthesis genes in AZC66 may help plants resist osmotic and salt stress. The discovery of glycine betaine, cold shock, and heat shock protein genes demonstrated that AZC66 could withstand harsh conditions. AZC66 might be used to create robust, sustainable biological fertilizers for future agricultural use in Saudi Arabia. Furthermore, the predicted adaptable metabolic pathways might serve as the basis for potential biotechnological applications in agriculture and industry.
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Claims 17
Claim · model-written summary
Inoculation with AZC66 significantly increased cowpea root and shoot dry weights in vitro
- Taxon
- cowpea
Vigna unguiculatae0 - Treatment
- in vitro inoculation with AZC66e1
- Interactor
- AZC66e1symbiont
- Outcome
- root and shoot dry weightse1
- Direction
- increasede1
- Setting
- in vitroe1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“Cowpea’s root and shoot dry weights were also significantly increased after in vitro inoculation with AZC66”
Claim · model-written summary
AZC66 can fix nitrogen
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e4symbiont
- Outcome
- fix nitrogene4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e4“the ability of AZC66 to fix nitrogen”
Claim · model-written summary
AZC66 produced IAA at 66.33 ± 0.44 μg mL−1
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e4symbiont
- Compound
- IAAe2
- Outcome
- produce IAAe2
- Value
- 66.33 ± 0.44 μg mL−1e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e2“produce IAA (66.33 ± 0.44 μg mL−1)”
e4“the ability of AZC66 to fix nitrogen”
Claim · model-written summary
AZC66 showed ACC deaminase activity of 278.40 ± 21 mol -ketobutyrate mg−1 h−1
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e4symbiont
- Outcome
- activity of ACC deaminasee3
- Value
- 278.40 ± 21 mol -ketobutyrate mg−1 h−1e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e3“exhibit the activity of ACC deaminase (278.40 ± 21 mol -ketobutyrate mg−1 h−1)”
e4“the ability of AZC66 to fix nitrogen”
Claim · model-written summary
AZC66 solubilized inorganic phosphate
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e4symbiont
- Outcome
- solubilize inorganic phosphatee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e4“the ability of AZC66 to fix nitrogen”
e5“solubilize inorganic phosphate”
Claim · model-written summary
The AZC66 genome has 4840 predicted genes
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e6symbiont
- Outcome
- genes were predictede6
- Value
- 4840 genese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e6“The identity of AZC66 was confirmed as Priestia filamentosa, and 4840 genes were predicted in its genome”
Claim · model-written summary
The identity of AZC66 was confirmed as Priestia filamentosa
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e6symbiont
- Outcome
- identity of AZC66e6
- Qualifier
- confirmed as Priestia filamentosae6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e6“The identity of AZC66 was confirmed as Priestia filamentosa, and 4840 genes were predicted in its genome”
Claim · model-written summary
The top three KEGG pathways with the most genes in AZC66 are signaling and cellular processes, genetic information processing, and carbohydrate metabolism
- Taxon
- cowpeaVigna unguiculatae0
- Outcome
- the top three pathwayse7
- Qualifier
- signaling and cellular processes, genetic information processing, and carbohydrate metabolisme7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e7“the top three pathways wherein the maximum number of genes are involved are signaling and cellular processes, genetic information processing, and carbohydrate metabolism”
Claim · model-written summary
The AZC66 genome contains nifU, implicated in nitrogen fixation
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Gene
- nifUe1
- Outcome
- nitrogen fixatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“The genome sequencing of the strain AZC66 revealed a number of genes implicated in plant biostimulation activities such as nitrogen fixation (nifU), phytohormone synthesis (trpAB genes), phosphate solubilization (PhbCEF, pstABCS, and phoU), and siderophore formation (FbpA, feoAB, and fetB)”
Claim · model-written summary
The AZC66 genome contains trpAB genes for phytohormone synthesis
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Gene
- trpAB genese1
- Outcome
- phytohormone synthesise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“The genome sequencing of the strain AZC66 revealed a number of genes implicated in plant biostimulation activities such as nitrogen fixation (nifU), phytohormone synthesis (trpAB genes), phosphate solubilization (PhbCEF, pstABCS, and phoU), and siderophore formation (FbpA, feoAB, and fetB)”
Claim · model-written summary
The AZC66 genome contains PhbCEF, pstABCS and phoU genes for phosphate solubilization
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Gene
- PhbCEF, pstABCS, and phoUe1
- Outcome
- phosphate solubilizatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“The genome sequencing of the strain AZC66 revealed a number of genes implicated in plant biostimulation activities such as nitrogen fixation (nifU), phytohormone synthesis (trpAB genes), phosphate solubilization (PhbCEF, pstABCS, and phoU), and siderophore formation (FbpA, feoAB, and fetB)”
Claim · model-written summary
The AZC66 genome contains FbpA, feoAB and fetB genes for siderophore formation
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Gene
- FbpA, feoAB, and fetBe1
- Outcome
- siderophore formatione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“The genome sequencing of the strain AZC66 revealed a number of genes implicated in plant biostimulation activities such as nitrogen fixation (nifU), phytohormone synthesis (trpAB genes), phosphate solubilization (PhbCEF, pstABCS, and phoU), and siderophore formation (FbpA, feoAB, and fetB)”
Claim · model-written summary
The AZC66 genome contains numerous genes involved in nitrogen metabolism and nitrate reduction
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Outcome
- genes involved in nitrogen metabolisme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“The AZC66 genome contained numerous genes involved in nitrogen metabolism, nitrogen regulation, and the nitrate reduction pathway”
Claim · model-written summary
The phenazine biosynthetic gene in AZC66 is associated with biocontrol and soil survival properties
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Gene
- phenazine biosynthetic genee1
- Outcome
- biocontrol and soil survival propertiese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“The phenazine biosynthetic gene in AZC66 demonstrated biocontrol and soil survival properties”
Claim · model-written summary
Trehalose synthesis genes in AZC66 may help plants resist osmotic and salt stress
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Gene
- trehalose synthesis genese1
- Outcome
- resist osmotic and salt stresse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“The trehalose synthesis genes in AZC66 may help plants resist osmotic and salt stress”
Claim · model-written summary
AZC66 genes for glycine betaine, cold shock and heat shock proteins indicate it could withstand harsh conditions
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Gene
- glycine betaine, cold shock, and heat shock protein genese1
- Outcome
- could withstand harsh conditionse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“The discovery of glycine betaine, cold shock, and heat shock protein genes demonstrated that AZC66 could withstand harsh conditions”
Claim · model-written summary
AZC66 might be used to create robust, sustainable biological fertilizers for agriculture in Saudi Arabia
- Taxon
- cowpeaVigna unguiculatae0
- Interactor
- AZC66e1symbiont
- Outcome
- create robust, sustainable biological fertilizerse1
- Setting
- Saudi Arabiae1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“determine its biostimulating effects on the growth of cowpea under greenhouse conditions”
e1“AZC66 might be used to create robust, sustainable biological fertilizers for future agricultural use in Saudi Arabia”
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