Abstract
Protective role of Trifloxystrobin pretreatment against excessive copper-related abiotic stress in Triticum aestivum L. was determined in two Turkish wheat cultivars, Sönmez and Gerek 79. Ten-day-old seedlings were pretreated with 20 µM and 80 µM Trifloxystrobin. A group of seedlings was harvested without exposure to Trifloxystrobin as a control. Two days after, seedlings were treated with copper(II) chloride. Seedlings were harvested on the 20th day after sowing. The growth level of the groups was evaluated by measuring the length, fresh weight and shoot dry weight of shoots. Chlorophyll a + b, carotenoid and anthocyanin contents as well as proline levels were assessed. Lipid peroxidation and total activities of superoxide dismutase, peroxidase and glutathione reductase were analyzed to predict the oxidative stress levels. Both cultivars exhibited similar responses to the treatments. Trifloxystrobin doses did not cause damage on plants when applied alone. Seedlings subjected to excessive doses of copper showed significant reductions in growth parameters, as well as chlorophyll and carotenoid pigments. Conversely, copper caused a remarkable increase in anthocyanin, proline and malondialdehyde accumulation. Superoxide dismutase and peroxidase activities increased, while glutathione reductase activity decreased in copper-stressed plants. Trifloxystrobin pretreatment strengthens the antioxidant defense system. All parameters were positively affected by Trifloxystrobin pretreatment. As the dose of Trifloxystrobin increased, the severity of stress decreased in both genotypes. Trifloxystrobin pretreatment is a promising method for reducing copper-induced damage in T. aestivum.
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Claims 11
Claim · model-written summary
Both wheat cultivars responded similarly to treatments.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Outcome
- similar responsese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Both cultivars exhibited similar responses to the treatments.”
t0“Triticum aestivum L.”
Claim · model-written summary
Trifloxystrobin alone did not damage plants.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- Trifloxystrobin dosese0
- Outcome
- damage on plantse0
- Direction
- did not causee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Trifloxystrobin doses did not cause damage on plants when applied alone.”
t0“Triticum aestivum L.”
Claim · model-written summary
Excessive copper significantly reduced seedling growth parameters.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- excessive doses of coppere0
- Outcome
- growth parameterse0
- Direction
- reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Seedlings subjected to excessive doses of copper showed significant reductions in growth parameters, as well as chlorophyll and carotenoid pigments.”
t0“Triticum aestivum L.”
Claim · model-written summary
Excessive copper significantly reduced chlorophyll and carotenoid pigments.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- excessive doses of coppere0
- Outcome
- chlorophyll and carotenoid pigmentse0
- Direction
- reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Seedlings subjected to excessive doses of copper showed significant reductions in growth parameters, as well as chlorophyll and carotenoid pigments.”
t0“Triticum aestivum L.”
Claim · model-written summary
Copper increased anthocyanin, proline and malondialdehyde accumulation.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- coppere0
- Outcome
- anthocyanin, proline and malondialdehyde accumulatione0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“copper caused a remarkable increase in anthocyanin, proline and malondialdehyde accumulation.”
t0“Triticum aestivum L.”
Claim · model-written summary
Copper stress increased superoxide dismutase and peroxidase activities.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- copper-stressede0
- Gene
- Superoxide dismutasee0
- Outcome
- Superoxide dismutase and peroxidase activitiese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Superoxide dismutase and peroxidase activities increased, while glutathione reductase activity decreased in copper-stressed plants.”
t0“Triticum aestivum L.”
Claim · model-written summary
Copper stress decreased glutathione reductase activity.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- copper-stressede0
- Gene
- glutathione reductasee0
- Outcome
- glutathione reductase activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“glutathione reductase activity decreased in copper-stressed plants.”
t0“Triticum aestivum L.”
Claim · model-written summary
Trifloxystrobin pretreatment strengthened antioxidant defense.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- Trifloxystrobin pretreatmente0
- Outcome
- antioxidant defense systeme0
- Direction
- strengthense0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Trifloxystrobin pretreatment strengthens the antioxidant defense system.”
t0“Triticum aestivum L.”
Claim · model-written summary
Trifloxystrobin pretreatment positively affected all measured parameters.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- Trifloxystrobin pretreatmente0
- Outcome
- All parameterse0
- Direction
- positively affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“All parameters were positively affected by Trifloxystrobin pretreatment.”
t0“Triticum aestivum L.”
Claim · model-written summary
Higher trifloxystrobin dose reduced stress severity in both genotypes.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- dose of Trifloxystrobine0
- Outcome
- severity of stresse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“As the dose of Trifloxystrobin increased, the severity of stress decreased in both genotypes.”
t0“Triticum aestivum L.”
Claim · model-written summary
The authors propose pretreatment as a way to reduce copper damage in wheat.
- Taxon
- T. aestivum
Triticum aestivume0 - Outcome
- Trifloxystrobin pretreatment is a promising method for reducing copper-induced damagee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Trifloxystrobin pretreatment is a promising method for reducing copper-induced damage in T. aestivum.”
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