Abstract
The determination of starch granule morphology in plants is poorly understood. The amyloplasts of wheat endosperm contain large discoid A-type granules and small spherical B-type granules. To study the influence of amyloplast structure on these distinct morphological types, we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm. Endosperm amyloplasts of the mutant contained more A- and B-type granules than those of the wild-type. The mutant had increased A- and B-type granule size in mature grains, and its A-type granules had a highly aberrant, lobed surface. This morphological defect was already evident at early stages of grain development and occurred without alterations in polymer structure and composition. Plant growth and grain size, number and starch content were not affected in the mutants despite the large plastid size. Interestingly, mutation of the PARC6 paralog, ARC6, did not increase plastid or starch granule size. We suggest TtPARC6 can complement disrupted TtARC6 function by interacting with PDV2, the outer plastid envelope protein that typically interacts with ARC6 to promote plastid division. We therefore reveal an important role of amyloplast structure in starch granule morphogenesis in wheat.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
The PARC6 mutant durum wheat endosperm amyloplasts contained more A- and B-type granules than the wild-type.
- Taxon
- durum wheat
Triticum turgidum subsp. durume1 - Organ / stage
- Endosperm amyloplastse2
- Treatment
- defective in the plastid division protein PARC6e1
- Gene
- PARC6e1
- Outcome
- A- and B-type granulese2
- Direction
- moree2
- Comparator
- wild-typee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm”
e2“Endosperm amyloplasts of the mutant contained more A- and B-type granules than those of the wild-type”
Claim · model-written summary
The PARC6 mutant has increased A- and B-type granule size in mature grains.
- Taxon
- durum wheatTriticum turgidum subsp. durume1
- Organ / stage
- mature grainse3
- Treatment
- defective in the plastid division protein PARC6e1
- Gene
- PARC6e1
- Outcome
- A- and B-type granule sizee3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm”
e3“The mutant had increased A- and B-type granule size in mature grains, and its A-type granules had a highly aberrant, lobed surface”
Claim · model-written summary
A-type granules of the PARC6 mutant have a highly aberrant, lobed surface.
- Taxon
- durum wheatTriticum turgidum subsp. durume1
- Gene
- PARC6e1
- Outcome
- highly aberrant, lobed surfacee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm”
e3“The mutant had increased A- and B-type granule size in mature grains, and its A-type granules had a highly aberrant, lobed surface”
Claim · model-written summary
The morphological defect of the PARC6 mutant is already evident at early stages of grain development.
- Taxon
- durum wheatTriticum turgidum subsp. durume1
- Organ / stage
- early stages of grain developmente4
- Outcome
- morphological defecte4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm”
e4“This morphological defect was already evident at early stages of grain development and occurred without alterations in polymer structure and composition”
Claim · model-written summary
The PARC6 mutant morphological defect occurs without alterations in starch polymer structure and composition.
- Taxon
- durum wheatTriticum turgidum subsp. durume1
- Outcome
- polymer structure and compositione4
- Direction
- without alterationse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm”
e4“This morphological defect was already evident at early stages of grain development and occurred without alterations in polymer structure and composition”
Claim · model-written summary
Plant growth, grain size, grain number and starch content are not affected in the PARC6 mutant.
- Taxon
- durum wheatTriticum turgidum subsp. durume1
- Treatment
- defective in the plastid division protein PARC6e1
- Outcome
- Plant growth and grain size, number and starch contente5
- Direction
- not affectede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm”
e5“Plant growth and grain size, number and starch content were not affected in the mutants despite the large plastid size”
Claim · model-written summary
Mutation of the PARC6 paralog ARC6 does not increase plastid or starch granule size in wheat.
- Taxon
- durum wheatTriticum turgidum subsp. durume1
- Treatment
- mutation of the PARC6 paralog, ARC6e6
- Gene
- ARC6e6
- Outcome
- plastid or starch granule sizee6
- Direction
- did not increasee6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm”
e6“Interestingly, mutation of the PARC6 paralog, ARC6, did not increase plastid or starch granule size”
Claim · model-written summary
TtPARC6 may complement disrupted TtARC6 function by interacting with PDV2 in wheat.
- Taxon
- durum wheatTriticum turgidum subsp. durume1
- Gene
- TtPARC6e7
- Outcome
- complement disrupted TtARC6 functione7
- Qualifier
- by interacting with PDV2e7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“we isolated a mutant in durum wheat (Triticum turgidum) defective in the plastid division protein PARC6, which had giant plastids in both leaves and endosperm”
e7“We suggest TtPARC6 can complement disrupted TtARC6 function by interacting with PDV2, the outer plastid envelope protein that typically interacts with ARC6 to promote plastid division”
Claim · model-written summary
Amyloplast structure plays an important role in starch granule morphogenesis in wheat.
- Taxon
- wheat
Triticum aestivume0 - Outcome
- starch granule morphogenesise0
- Qualifier
- amyloplast structuree0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We therefore reveal an important role of amyloplast structure in starch granule morphogenesis in wheat”
The same questions in other papers
loss of function · quality: sugar starch · Triticum turgidum (rivet wheat)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.