Abstract
Cotton is an important natural fiber crop. The RF2 gene family is a member of the bZIP transcription factor superfamily, which plays an important role in plant resistance to environmental stresses. In this paper, the RF2 gene family of four cotton species was analyzed genome-wide, and the key gene RF2-32 was cloned for functional verification. A total of 113 RF2 genes were identified in the four cotton species, and the RF2 family was relatively conserved during the evolution of cotton. Chromosome mapping and collinear analysis indicated that fragment replication was the main expansion mode of RF2 gene family during evolution. Cis-element analysis showed that there were many elements related to light response, hormone response and abiotic stress response in the promoters of RF2 genes. The transcriptome and qRT-PCR analysis of RF2 family genes in upland cotton showed that RF2 family genes responded to salt stress and drought stress. GhRF2-32 protein was localized in the cell nucleus. Silencing the GhRF2-32 gene showed less leaf wilting and increased total antioxidant capacity under drought and salt stress, decreased malondialdehyde content and increased drought and salt tolerance. This study revealed the evolutionary and functional diversity of the RF2 gene family, which laid a foundation for the further study of stress-resistant genes in cotton.
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Claims 10
Claim · model-written summary
113 RF2 genes were identified in four cotton species.
- Taxon
- cotton
Gossypium hirsutume2 - Outcome
- RF2 genes were identifiede2
- Value
- 113e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“A total of 113 RF2 genes were identified in the four cotton species”
Claim · model-written summary
The RF2 family was relatively conserved during cotton evolution.
- Taxon
- cottonGossypium hirsutume3
- Outcome
- relatively conservede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“the RF2 family was relatively conserved during the evolution of cotton”
Claim · model-written summary
Fragment replication was the main expansion mode of the cotton RF2 gene family.
- Taxon
- CottonGossypium hirsutume0
- Outcome
- main expansion mode of RF2 gene familye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton is an important natural fiber crop”
e4“fragment replication was the main expansion mode of RF2 gene family during evolution”
Claim · model-written summary
RF2 gene promoters contain many elements related to light, hormone and abiotic stress responses.
- Taxon
- CottonGossypium hirsutume0
- Outcome
- elements related to light response, hormone response and abiotic stress responsee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton is an important natural fiber crop”
e5“there were many elements related to light response, hormone response and abiotic stress response in the promoters of RF2 genes”
Claim · model-written summary
RF2 family genes in upland cotton responded to salt stress and drought stress.
Verbatim
e6“The transcriptome and qRT-PCR analysis of RF2 family genes in upland cotton showed that RF2 family genes responded to salt stress and drought stress”
Claim · model-written summary
The GhRF2-32 protein was localized in the cell nucleus.
- Taxon
- CottonGossypium hirsutume0
- Gene
- GhRF2-32e7
- Outcome
- localized in the cell nucleuse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton is an important natural fiber crop”
e7“GhRF2-32 protein was localized in the cell nucleus”
Claim · model-written summary
Silencing GhRF2-32 in cotton gave less leaf wilting under drought and salt stress.
- Taxon
- CottonGossypium hirsutume0
- Treatment
- Silencing the GhRF2-32 genee1
- Gene
- GhRF2-32e1
- Outcome
- leaf wiltinge1
- Direction
- lesse1
- Qualifier
- under drought and salt stresse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton is an important natural fiber crop”
e1“Silencing the GhRF2-32 gene showed less leaf wilting and increased total antioxidant capacity under drought and salt stress, decreased malondialdehyde content and increased drought and salt tolerance”
Claim · model-written summary
Silencing GhRF2-32 in cotton increased total antioxidant capacity under drought and salt stress.
- Taxon
- CottonGossypium hirsutume0
- Treatment
- Silencing the GhRF2-32 genee1
- Outcome
- total antioxidant capacitye1
- Direction
- increasede1
- Qualifier
- under drought and salt stresse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton is an important natural fiber crop”
e1“Silencing the GhRF2-32 gene showed less leaf wilting and increased total antioxidant capacity under drought and salt stress, decreased malondialdehyde content and increased drought and salt tolerance”
Claim · model-written summary
Silencing GhRF2-32 in cotton decreased malondialdehyde content.
- Taxon
- CottonGossypium hirsutume0
- Treatment
- Silencing the GhRF2-32 genee1
- Outcome
- malondialdehyde contente1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton is an important natural fiber crop”
e1“Silencing the GhRF2-32 gene showed less leaf wilting and increased total antioxidant capacity under drought and salt stress, decreased malondialdehyde content and increased drought and salt tolerance”
Claim · model-written summary
Silencing GhRF2-32 in cotton increased drought and salt tolerance.
- Taxon
- CottonGossypium hirsutume0
- Treatment
- Silencing the GhRF2-32 genee1
- Outcome
- drought and salt tolerancee1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Cotton is an important natural fiber crop”
e1“Silencing the GhRF2-32 gene showed less leaf wilting and increased total antioxidant capacity under drought and salt stress, decreased malondialdehyde content and increased drought and salt tolerance”
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