Abstract
In plant cells, vacuolar H+-ATPases (V-ATPases) are responsible for deacidification of the cytosol and energisation of the secondary transport processes across the tonoplast. A number of V-ATPase subunit genes have been demonstrated to be involved in the regulation of the plant response to water deficit. However, there are no reports on the role of V-ATPase subunit A (VHA-A) in dehydration tolerance of cotton. In this study, cotton GhVHA-A gene was functionally characterised, especially with regard to its role in dehydration stress tolerance. Expression analysis showed that GhVHA-A was differentially expressed in various cotton organs and was induced by dehydration, low temperature, high salinity, and abscisic acid treatment in leaves. We also report that GhVHA-A improve dehydration tolerance in transgenic tobacco and cotton. Virus-induced gene silencing of GhVHA-A decreased the tolerance of cotton plantlets to dehydration stress. Silencing GhVHA-A decreased chlorophyll content and antioxidant enzyme activities and increased malondialdehyde (MDA) content in cotton under dehydration stress. However, transgenic tobacco expressing GhVHA-A exhibited enhanced dehydration resistance, resulting in reduced leaf water loss, higher average root length, and lower MDA levels under dehydration stress. Meanwhile, overexpression of GhVHA-A in tobacco conferred water deficit tolerance by enhancing osmotic adjustment (proline) and the activities of the antioxidant enzymes superoxide dismutase and peroxidase, thereby enhancing reactive oxygen species detoxification. These results suggest that GhVHA-A plays an important role in conferring resistance to dehydration stress. Our results have identified GhVHA-A as a candidate gene for improving dehydration tolerance in plants.
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Claims 11
Claim · model-written summary
GhVHA-A expression varied among cotton organs.
- Taxon
- Cotton
Gossypium hirsutumt0 - Gene
- GhVHA-Ae0
- Outcome
- differentially expressed in various cotton organse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhVHA-A was differentially expressed in various cotton organs”
t0“Cotton”
Claim · model-written summary
Dehydration, cold, salinity and abscisic acid induced GhVHA-A in cotton leaves.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- leavese0
- Treatment
- dehydration, low temperature, high salinity, and abscisic acid treatmente0
- Outcome
- inducede0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was induced by dehydration, low temperature, high salinity, and abscisic acid treatment in leaves”
t0“Cotton”
Claim · model-written summary
Silencing GhVHA-A decreased cotton dehydration tolerance.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Virus-induced gene silencing of GhVHA-Ae0
- Gene
- GhVHA-Ae0
- Outcome
- tolerance of cotton plantlets to dehydration stresse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Virus-induced gene silencing of GhVHA-A decreased the tolerance of cotton plantlets to dehydration stress”
t0“Cotton”
Claim · model-written summary
Silencing GhVHA-A decreased cotton chlorophyll content under dehydration.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Silencing GhVHA-Ae0
- Gene
- GhVHA-Ae0
- Outcome
- chlorophyll contente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GhVHA-A decreased chlorophyll content and antioxidant enzyme activities and increased malondialdehyde (MDA) content in cotton under dehydration stress”
t0“Cotton”
Claim · model-written summary
Silencing GhVHA-A decreased cotton antioxidant enzyme activities under dehydration.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Silencing GhVHA-Ae0
- Gene
- GhVHA-Ae0
- Outcome
- antioxidant enzyme activitiese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GhVHA-A decreased chlorophyll content and antioxidant enzyme activities and increased malondialdehyde (MDA) content in cotton under dehydration stress”
t0“Cotton”
Claim · model-written summary
Silencing GhVHA-A increased cotton MDA content under dehydration.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Silencing GhVHA-Ae0
- Gene
- GhVHA-Ae0
- Outcome
- malondialdehyde (MDA) contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing GhVHA-A decreased chlorophyll content and antioxidant enzyme activities and increased malondialdehyde (MDA) content in cotton under dehydration stress”
t0“Cotton”
Claim · model-written summary
GhVHA-A-expressing tobacco had reduced leaf water loss under dehydration.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- transgenic tobacco expressing GhVHA-Ae0
- Gene
- GhVHA-Ae0
- Outcome
- leaf water losse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic tobacco expressing GhVHA-A exhibited enhanced dehydration resistance, resulting in reduced leaf water loss, higher average root length, and lower MDA levels under dehydration stress”
t0“transgenic tobacco expressing GhVHA-A”
Claim · model-written summary
GhVHA-A-expressing tobacco had higher average root length under dehydration.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- transgenic tobacco expressing GhVHA-Ae0
- Gene
- GhVHA-Ae0
- Outcome
- average root lengthe0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic tobacco expressing GhVHA-A exhibited enhanced dehydration resistance, resulting in reduced leaf water loss, higher average root length, and lower MDA levels under dehydration stress”
t0“transgenic tobacco expressing GhVHA-A”
Claim · model-written summary
GhVHA-A-expressing tobacco had lower MDA under dehydration.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- transgenic tobacco expressing GhVHA-Ae0
- Gene
- GhVHA-Ae0
- Outcome
- MDA levelse0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic tobacco expressing GhVHA-A exhibited enhanced dehydration resistance, resulting in reduced leaf water loss, higher average root length, and lower MDA levels under dehydration stress”
t0“transgenic tobacco expressing GhVHA-A”
Claim · model-written summary
GhVHA-A overexpression enhanced tobacco osmotic adjustment through proline under water deficit.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- overexpression of GhVHA-Ae0
- Gene
- GhVHA-Ae0
- Outcome
- water deficit tolerancee0
- Qualifier
- enhancing osmotic adjustment (proline)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GhVHA-A in tobacco conferred water deficit tolerance by enhancing osmotic adjustment (proline)”
t0“overexpression of GhVHA-A in tobacco”
Claim · model-written summary
GhVHA-A overexpression increased superoxide dismutase and peroxidase activities, supporting reactive oxygen species detoxification.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- reactive oxygen species detoxificatione0
- Qualifier
- enhancing reactive oxygen species detoxificatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“enhancing reactive oxygen species detoxification”
t0“overexpression of GhVHA-A in tobacco”
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