Paper · 2023 · Natural and Life Sciences Communications

Antioxidant, Antibacterial Activities and Cytotoxicity of Garlic Leaf Extract from Garlic Waste

Panee Sirisa-ard; Kiatisak Pholsonklam; Apinya Satchachai; et al.

16
claims
29
verbatim quotes
4
citations
1
research areas
—
Not open access

Abstract

Five garlic parts (root, clove skin, leaf, flower stalk and garlic peel) of garlic waste from garlic harvest processing were used in this study. Each part of the garlic waste was screened for total phenolic, flavonoid and antioxidant activity after extraction with 70% ethanol. Interestingly, garlic leaf (GLf) showed the greatest richness of total phenolic, flavonoid and antioxidant activity. GLf was selected to extract with water and 70% ethanol and the highest extraction yield was achieved with water extract. The GLf extracts demonstrated a good quality standard of physical and chemical properties. The aqueous extract of GLf revealed the highest total phenolic content of 1,676.40 ± 18.02 mg GAE/100 g extract. Both aqueous and ethanolic extracts did not differ regarding total flavonoid contents, showing values of 118.60 ± 6.10 mg QUE/100 g extract and 110.90 ± 2.30 mg QUE/100 g extract, respectively. Phenolic compounds including gallic acid, catechin and tannic acid were detected in the aqueous and ethanolic extracts of GLf by HPLC assay. Moreover, four flavonoid compounds including isoquercetin, quercetin, kaempferol and rutin were detected in aqueous extract whereas the ethanolic extract presented apigenin, isoquercetin, quercetin and kaempferol. The ethanolic extract of GLf demonstrated the stronger antioxidant activity than aqueous extract. GLf extracts also exhibited antimicrobial activity against pathogenic bacteria with MIC and MBC values of 125-250 mg/mL. In addition, GLf aqueous extract showed lower toxicity on Vero cells than ethanolic extract. Therefore, GLf waste can be used as a cheap source of natural compounds and antioxidants, with potential applications in different biological, medicinal and food products. Keywords: Allium sativum L., Antibacteria, Antioxidant, Garlic, Toxicity

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 16

Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic leaf had the greatest total phenolic content among waste parts.

Taxon
garlicAllium sativume0
Organ / stage
leafe0
Outcome
total phenolice0
Direction
greateste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“garlic leaf (GLf) showed the greatest richness of total phenolic, flavonoid and antioxidant activity”

✓ located verbatim in the source · abstract, characters 293–393 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic leaf had the greatest flavonoid content among waste parts.

Taxon
garlicAllium sativume0
Organ / stage
leafe0
Outcome
flavonoide0
Direction
greateste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“garlic leaf (GLf) showed the greatest richness of total phenolic, flavonoid and antioxidant activity”

✓ located verbatim in the source · abstract, characters 293–393 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic leaf had the greatest antioxidant activity among waste parts.

Taxon
garlicAllium sativume0
Organ / stage
leafe0
Outcome
antioxidant activitye0
Direction
greateste0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“garlic leaf (GLf) showed the greatest richness of total phenolic, flavonoid and antioxidant activity”

✓ located verbatim in the source · abstract, characters 293–393 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Water extraction gave the highest yield from garlic leaf.

Taxon
GarlicAllium sativumt0
Treatment
water extracte0
Outcome
extraction yielde0
Direction
higheste0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the highest extraction yield was achieved with water extract”

✓ located verbatim in the source · abstract, characters 454–514 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic leaf extracts met a good standard for physical and chemical properties.

Taxon
GarlicAllium sativumt0
Organ / stage
Leaft0
Outcome
physical and chemical propertiese0
Qualifier
good quality standarde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The GLf extracts demonstrated a good quality standard of physical and chemical properties”

✓ located verbatim in the source · abstract, characters 516–605 · show in text ↑
t0

“Garlic Leaf”

✓ located verbatim in the source · title, characters 58–69 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic leaf aqueous extract had the highest total phenolic content, 1,676.40 ± 18.02 mg GAE/100 g extract.

Taxon
GarlicAllium sativumt0
Organ / stage
Leaft0
Outcome
total phenolic contente0
Direction
higheste0
Value
1,676.40 ± 18.02 mg GAE/100 g extracte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The aqueous extract of GLf revealed the highest total phenolic content of 1,676.40 ± 18.02 mg GAE/100 g extract”

✓ located verbatim in the source · abstract, characters 607–718 · show in text ↑
t0

“Garlic Leaf”

✓ located verbatim in the source · title, characters 58–69 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Garlic leaf aqueous and ethanolic extracts did not differ in total flavonoid content.

Taxon
GarlicAllium sativumt0
Treatment
aqueous and ethanolic extractse0
Outcome
total flavonoid contentse0
Direction
did not differe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both aqueous and ethanolic extracts did not differ regarding total flavonoid contents, showing values of 118.60 ± 6.10 mg QUE/100 g extract and 110.90 ± 2.30 mg QUE/100 g extract, respectively”

✓ located verbatim in the source · abstract, characters 720–912 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Aqueous garlic leaf extract had 118.60 ± 6.10 mg QUE/100 g extract of total flavonoids.

Taxon
GarlicAllium sativumt0
Treatment
aqueouse0
Outcome
total flavonoid contentse0
Value
118.60 ± 6.10 mg QUE/100 g extracte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both aqueous and ethanolic extracts did not differ regarding total flavonoid contents, showing values of 118.60 ± 6.10 mg QUE/100 g extract and 110.90 ± 2.30 mg QUE/100 g extract, respectively”

✓ located verbatim in the source · abstract, characters 720–912 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ethanolic garlic leaf extract had 110.90 ± 2.30 mg QUE/100 g extract of total flavonoids.

Taxon
GarlicAllium sativumt0
Treatment
ethanolice0
Outcome
total flavonoid contentse0
Value
110.90 ± 2.30 mg QUE/100 g extracte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Both aqueous and ethanolic extracts did not differ regarding total flavonoid contents, showing values of 118.60 ± 6.10 mg QUE/100 g extract and 110.90 ± 2.30 mg QUE/100 g extract, respectively”

✓ located verbatim in the source · abstract, characters 720–912 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Gallic acid, catechin, and tannic acid were detected in aqueous and ethanolic garlic leaf extracts.

Taxon
GarlicAllium sativumt0
Organ / stage
Leaft0
Outcome
gallic acid, catechin and tannic acid were detectede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Phenolic compounds including gallic acid, catechin and tannic acid were detected in the aqueous and ethanolic extracts of GLf”

✓ located verbatim in the source · abstract, characters 914–1039 · show in text ↑
t0

“Garlic Leaf”

✓ located verbatim in the source · title, characters 58–69 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Isoquercetin, quercetin, kaempferol, and rutin were detected in aqueous garlic leaf extract.

Taxon
GarlicAllium sativumt0
Outcome
isoquercetin, quercetin, kaempferol and rutin were detectede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“four flavonoid compounds including isoquercetin, quercetin, kaempferol and rutin were detected in aqueous extract”

✓ located verbatim in the source · abstract, characters 1065–1178 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Apigenin, isoquercetin, quercetin, and kaempferol were detected in ethanolic garlic leaf extract.

Taxon
GarlicAllium sativumt0
Outcome
apigenin, isoquercetin, quercetin and kaempferole0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the ethanolic extract presented apigenin, isoquercetin, quercetin and kaempferol”

✓ located verbatim in the source · abstract, characters 1187–1267 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ethanolic garlic leaf extract had stronger antioxidant activity than aqueous extract.

Taxon
GarlicAllium sativumt0
Treatment
ethanolic extracte0
Outcome
antioxidant activitye0
Direction
strongere0
Comparator
aqueous extracte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The ethanolic extract of GLf demonstrated the stronger antioxidant activity than aqueous extract”

✓ located verbatim in the source · abstract, characters 1269–1365 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Garlic leaf extracts inhibited pathogenic bacteria with MIC and MBC values of 125-250 mg/mL.

Taxon
GarlicAllium sativumt0
Interactor
pathogenic bacteriae0other
Outcome
antimicrobial activitye0
Value
125-250 mg/mLe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“GLf extracts also exhibited antimicrobial activity against pathogenic bacteria with MIC and MBC values of 125-250 mg/mL”

✓ located verbatim in the source · abstract, characters 1367–1486 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Aqueous garlic leaf extract was less toxic to Vero cells than ethanolic extract.

Taxon
GarlicAllium sativumt0
Treatment
aqueous extracte0
Outcome
toxicity on Vero cellse0
Direction
lowere0
Comparator
ethanolic extracte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“GLf aqueous extract showed lower toxicity on Vero cells than ethanolic extract”

✓ located verbatim in the source · abstract, characters 1501–1579 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors propose garlic leaf waste as a source of natural compounds and antioxidants for biological, medicinal, and food products.

Taxon
GarlicAllium sativumt0
Outcome
cheap source of natural compounds and antioxidantse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium sativum
Allium sativum · TaxonIvar Leidus · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“GLf waste can be used as a cheap source of natural compounds and antioxidants, with potential applications in different biological, medicinal and food products”

✓ located verbatim in the source · abstract, characters 1592–1751 · show in text ↑
t0

“Garlic Leaf Extract”

✓ located verbatim in the source · title, characters 58–77 · show in text ↑

botanical · pest · Allium sativum (garlic)Positive and negative results

4 papers: 3 positive, 1 negative or no effect

2 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive
See all 4 papers →

botanical · yield · Allium sativum (garlic)Positive and negative results

25 papers: 23 positive, 2 negative or no effect

22 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. no effect
  3. positive

21 papers more in this group

See all 25 papers →

botanical · quality: phytochemicals · Allium sativum (garlic)Results agree

9 papers: 9 positive, 0 negative or no effect

8 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

5 papers more in this group

See all 9 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.