Paper · 2023 · Plants

Inoculation with Azospirillum brasilense Strains AbV5 and AbV6 Increases Nutrition, Chlorophyll, and Leaf Yield of Hydroponic Lettuce

Carlos Eduardo da Silva Oliveira; Arshad Jalal; Letícia Schenaide Vitória; et al.

11
claims
37
verbatim quotes
33
citations
3
research areas
✓
Open access · cc-by

Abstract

Inoculation with Azospirillum brasilense has promisingly increased plant yield and nutrient acquisition. The study aimed to estimate the dose of A. brasilense that increases yield, gas exchange, nutrition, and foliar nitrate reduction. The research was carried out in a greenhouse at Ilha Solteira, in a hydroponic system in randomized blocks with four replicates. The treatments consisted of doses of inoculation with A. brasilense strains AbV5 and AbV6 via nutrient solution (0, 8, 16, 32, and 64 mL 100 L−1). Inoculation with A. brasilense at calculated doses between 20 and 44 mL provided the highest fresh and dry mass of shoots and roots, number of leaves, and leaf yield. In addition, the calculated doses of inoculation with A. brasilense increased the accumulation of N, P, K, Ca, Mg, S, B, Fe, Mn, and Zn in shoots and roots, except the accumulation of Ca in roots. It also increased cell membrane integrity index (15%), relative water content (13%), net photosynthesis rate (85%), intracellular CO2 concentration (15%), total chlorophyll (46%), stomatal conductance (56%), transpiration (15%), and water use efficiency (59%). Hence, inoculation with A. brasilense at doses between 20 and 44 mL 100 L−1 is considered the best approach for increasing the growth, yield, accumulation of nutrients, and gas exchange of hydroponically grown iceberg lettuce.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 11

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation at 20 to 44 mL maximized shoot and root fresh and dry mass, leaf number, and leaf yield.

Taxon
LettuceLactuca sativat0
Treatment
Inoculation with A. brasilense at calculated doses between 20 and 44 mLe1
Interactor
A. brasilenseAzospirillum brasilensee1symbiont
Outcome
fresh and dry mass of shoots and roots, number of leaves, and leaf yielde1
Direction
higheste1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e1

“Inoculation with A. brasilense at calculated doses between 20 and 44 mL provided the highest fresh and dry mass of shoots and roots, number of leaves, and leaf yield.”

✓ located verbatim in the source · abstract, characters 512–678 · show in text ↑
e2

“A. brasilense strains AbV5 and AbV6 via nutrient solution (0, 8, 16, 32, and 64 mL 100 L−1)”

✓ located verbatim in the source · abstract, characters 419–510 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased shoot and root accumulation of N, P, K, Ca, Mg, S, B, Fe, Mn, and Zn, except root Ca.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee0
Interactor
A. brasilenseAzospirillum brasilensee0symbiont
Outcome
accumulation of N, P, K, Ca, Mg, S, B, Fe, Mn, and Zn in shoots and rootse0
Direction
increasede0
Qualifier
except the accumulation of Ca in rootse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“the calculated doses of inoculation with A. brasilense increased the accumulation of N, P, K, Ca, Mg, S, B, Fe, Mn, and Zn in shoots and roots, except the accumulation of Ca in roots”

✓ located verbatim in the source · abstract, characters 692–874 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased cell membrane integrity index by 15%.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee1
Interactor
A. brasilenseAzospirillum brasilensee1symbiont
Outcome
cell membrane integrity indexe0
Direction
increasede0
Value
15%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“It also increased cell membrane integrity index (15%)”

✓ located verbatim in the source · abstract, characters 876–929 · show in text ↑
e1

“calculated doses of inoculation with A. brasilense increased”

✓ located verbatim in the source · abstract, characters 696–756 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased relative water content by 13%.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee1
Interactor
A. brasilenseAzospirillum brasilensee1symbiont
Outcome
relative water contente0
Direction
increasede0
Value
13%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“It also increased cell membrane integrity index (15%), relative water content (13%)”

✓ located verbatim in the source · abstract, characters 876–959 · show in text ↑
e1

“calculated doses of inoculation with A. brasilense increased”

✓ located verbatim in the source · abstract, characters 696–756 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased net photosynthesis rate by 85%.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee2
Interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
net photosynthesis ratee0
Direction
increasede1
Value
85%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“net photosynthesis rate (85%)”

✓ located verbatim in the source · abstract, characters 961–990 · show in text ↑
e1

“It also increased cell membrane integrity index (15%)”

✓ located verbatim in the source · abstract, characters 876–929 · show in text ↑
e2

“calculated doses of inoculation with A. brasilense increased”

✓ located verbatim in the source · abstract, characters 696–756 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased intracellular CO2 concentration by 15%.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee2
Interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
intracellular CO2 concentratione0
Direction
increasede1
Value
15%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“intracellular CO2 concentration (15%)”

✓ located verbatim in the source · abstract, characters 992–1029 · show in text ↑
e1

“It also increased cell membrane integrity index (15%)”

✓ located verbatim in the source · abstract, characters 876–929 · show in text ↑
e2

“calculated doses of inoculation with A. brasilense increased”

✓ located verbatim in the source · abstract, characters 696–756 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased total chlorophyll by 46%.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee2
Interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
total chlorophylle0
Direction
increasede1
Value
46%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“total chlorophyll (46%)”

✓ located verbatim in the source · abstract, characters 1031–1054 · show in text ↑
e1

“It also increased cell membrane integrity index (15%)”

✓ located verbatim in the source · abstract, characters 876–929 · show in text ↑
e2

“calculated doses of inoculation with A. brasilense increased”

✓ located verbatim in the source · abstract, characters 696–756 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased stomatal conductance by 56%.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee2
Interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
stomatal conductancee0
Direction
increasede1
Value
56%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“stomatal conductance (56%)”

✓ located verbatim in the source · abstract, characters 1056–1082 · show in text ↑
e1

“It also increased cell membrane integrity index (15%)”

✓ located verbatim in the source · abstract, characters 876–929 · show in text ↑
e2

“calculated doses of inoculation with A. brasilense increased”

✓ located verbatim in the source · abstract, characters 696–756 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased transpiration by 15%.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee2
Interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
transpiratione0
Direction
increasede1
Value
15%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“transpiration (15%)”

✓ located verbatim in the source · abstract, characters 1084–1103 · show in text ↑
e1

“It also increased cell membrane integrity index (15%)”

✓ located verbatim in the source · abstract, characters 876–929 · show in text ↑
e2

“calculated doses of inoculation with A. brasilense increased”

✓ located verbatim in the source · abstract, characters 696–756 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased water use efficiency by 59%.

Taxon
LettuceLactuca sativat0
Treatment
inoculation with A. brasilensee2
Interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
water use efficiencye0
Direction
increasede1
Value
59%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“water use efficiency (59%)”

✓ located verbatim in the source · abstract, characters 1109–1135 · show in text ↑
e1

“It also increased cell membrane integrity index (15%)”

✓ located verbatim in the source · abstract, characters 876–929 · show in text ↑
e2

“calculated doses of inoculation with A. brasilense increased”

✓ located verbatim in the source · abstract, characters 696–756 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors recommend 20 to 44 mL per 100 L Azospirillum inoculation for hydroponic iceberg lettuce.

Taxon
lettuceLactuca sativae0
Treatment
inoculation with A. brasilense at doses between 20 and 44 mL 100 L−1e0
Interactor
A. brasilenseAzospirillum brasilensee0symbiont
Outcome
best approach for increasing the growth, yield, accumulation of nutrients, and gas exchangee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Lactuca sativa
Lactuca sativa · Taxonen:User:Geographer · CC BY 1.0 · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · InteractorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

t0

“Hydroponic Lettuce”

✓ located verbatim in the source · title, characters 115–133 · show in text ↑
e0

“inoculation with A. brasilense at doses between 20 and 44 mL 100 L−1 is considered the best approach for increasing the growth, yield, accumulation of nutrients, and gas exchange of hydroponically grown iceberg lettuce”

✓ located verbatim in the source · abstract, characters 1144–1362 · show in text ↑

Azospirillum brasilense · nutrient: content · Lactuca sativa (garden lettuce)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Azospirillum brasilense · yield · Lactuca sativa (garden lettuce)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

Azospirillum brasilense · molecular: metabolites · Lactuca sativa (garden lettuce)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Azospirillum brasilense · physiology: pigments · Lactuca sativa (garden lettuce)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

inoculation · yield · Lactuca sativa (garden lettuce)Results differ

36 papers: 32 positive, 4 negative or no effect

31 papers in the same direction as this paper · 4 in the opposite direction

  1. positive
  2. positive
  3. no effect

32 papers more in this group

See all 36 papers →

inoculation · physiology: gas exchange · Lactuca sativa (garden lettuce)Positive and negative results

5 papers: 4 positive, 1 negative or no effect

3 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

inoculation · molecular: metabolites · Lactuca sativa (garden lettuce)Positive and negative results

17 papers: 16 positive, 1 negative or no effect

15 papers in the same direction as this paper · 1 in the opposite direction

  1. negative
  2. positive
  3. positive

13 papers more in this group

See all 17 papers →

inoculation · physiology: pigments · Lactuca sativa (garden lettuce)Positive and negative results

20 papers: 18 positive, 2 negative or no effect

17 papers in the same direction as this paper · 2 in the opposite direction

  1. no effect
  2. negative
  3. positive

16 papers more in this group

See all 20 papers →

8 of 11 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.